Haplotype Effect of Two Human Leukocyte Antigen-G Polymorphisms of rs1736933 and rs2735022 on the Recurrent Pregnancy Loss

Zahra Najafi; mohammad khalaj-kondori; Mohammad Ali Hosseinpour Feizi; Shamsi Abbasaliizadeh

Journal of Inflammatory Diseases

The Official Journal of Qazvin University of Medical Sciences

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Haplotype Effect of Two Human Leukocyte Antigen-G Polymorphisms of rs1736933 and rs2735022 on the Recurrent Pregnancy Loss

Author(s):

Zahra Najafi¹,

mohammad khalaj-kondori

^1,*,

Mohammad Ali Hosseinpour Feizi ¹,

Shamsi Abbasaliizadeh²

1Department of Animal Sciences, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.

2Department of Gynecology ;Obstetrics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Journal of Inflammatory Diseases:Vol. 24, issue 5; 398-409

Article type:Research Article

How to Cite:Zahra Najafimohammad khalaj-kondoriMohammad Ali Hosseinpour Feizi Shamsi AbbasaliizadehHaplotype Effect of Two Human Leukocyte Antigen-G Polymorphisms of rs1736933 and rs2735022 on the Recurrent Pregnancy Loss.J Inflamm Dis.24(5):e156240.

Abstract

Background: Recurrent Pregnancy Loss (RPL) is a multifactorial disease that affects 1-3% of couples. Since Human Leukocyte Antigen-G (HLA-G) gene is involved in fetal maternal immune tolerance, mutations in the HLA-G gene can affect the success rate of pregnancy. Objective: The present study aims to investigate the haplotype effect of rs1736933 and rs2735022 polymorphisms found in the HLA-G gene on the RPL. Methods In this case-control study, participants were 100 women with RPL and 80 women with normal fertility in northwestern Iran. The HLA-G gene promoter was amplified by Polymerase Chain Reaction (PCR) method and sequenced. The genotype and allele frequencies of the two polymorphisms were compared between the two groups by using t-test in SPSS software version 22. Haplotype analysis was performed using PHASE 2.1 and Haploview 4.2 applications Findings: C allele and CC genotype in rs2735022 polymorphism and the G allele and GG genotype in rs1736933 polymorphism showed a significant association with the RPL (P

Keywords

Recurrent pregnancy loss

Polymorphism

Haplotype

Human Leukocyte antigen-G

References

1.
Ghanami Gashti N, Salehi Z, Zahiri Sorouri Z, Eskafi Sabet E. Analysis of 4977-bp mitochondrial DNA deletion in the women with spontaneous abortion. J Guilan Univ Med Sci. 2014; 22(88):1-6. [In Persian] http://journal.gums.ac.ir/article1-412-en.html.
2.
Keirse MJ, Rush RW, Anderson AB, Turnbull AC. Risk of preterm delivery in patients with previous pre-term delivery and/or abortion. Br J Obstet Gynaecol. 1978; 85(2):81-5. [DOI:10.1111/j.1471-0528.1978.tb10457.x] [PMID].
3.
Alemayehu M, Yebyo H, Medhanyie AA, Bayray A, Fantahun M, Goba GK. Determinants of repeated abortion among women of reproductive age attending health facilities in Northern Ethiopia: A case-control study. BMC Public Health. 2017; 17(1):188. [DOI:10.1186/s12889-017-4106-1] [PMID] [PMCID].
4.
Carosella ED, Moreau P, Lemaoult J, Rouas-Freiss N. HLAG: From biology to clinical benefits. Trends Immunol. 2008; 29(3):125-32. [DOI:10.1016/j.it.2007.11.005] [PMID].
5.
Pokale YS. Recurrent miscarriage. Int Res J Med Sci. 2015; 3(9):13-9. http://www.isca.in/MEDI_SCI/Archive/v3/i9/2. ISCA-IRJMedS-2015-036.php.
6.
Vaiman D. Genetic regulation of recurrent spontaneous abortion in humans. Biomed J. 2015; 38(1):11-24. [DOI:10.4103/2319-4170.133777] [PMID].
7.
Le Bouteiller P, Sargent IL. HLA class I molecules in the placenta: which ones, where and what for? A workshop report. Placenta. 2000; 21 Suppl A:S93-6. [DOI:10.1053/plac.1999.0530] [PMID].
8.
Carosella ED, LeMaoult J. HLA-G: A look back, a look forward. Cell Mol Life Sci. 2011; 68(3):337-40. [DOI:10.1007/s00018010-0577-2] [PMID].
9.
Shah I, hman E. Unsafe abortion: Global and regional incidence, trends, consequences, and challenges. J Obstet Gynaecol Can. 2009; 31(12):1149-58. [DOI:10.1016/S17012163(16)34376-6].
10.
Daher S, Mattar R, Gueuvoghlanian-Silva BY, Torloni MR. Genetic polymorphisms and recurrent spontaneous abortions: An overview of current knowledge. Am J Reprod Immunol. 2012; 67(4):341-7. [DOI:10.1111/j.1600-0897.2012.01123.x] [PMID].
11.
Ellis SA, Sargent IL, Redman CW, McMichael AJ. Evidence for a novel HLA antigen found on human extravillous trophoblast and a choriocarcinoma cell line. Immunology. 1986; 59(4):595601. [PMID] [PMCID].
12.
Ferreira LMR, Meissner TB, Tilburgs T, Strominger JL. HLA-G: At the interface of maternal-fetal tolerance. Trends Immunol. 2017; 38(4):272-86. [DOI:10.1016/j.it.2017.01.009] [PMID].
13.
Craenmehr MHC, Nederlof I, Cao M, Drabbels JJM, SpruytGerritse MJ, Anholts JDH, et al. Increased HLA-G expression in term placenta of women with a history of recurrent miscarriage despite their genetic predisposition to decreased HLA-G levels. Int J Mol Sci. 2019; 20(3):625. [DOI:10.3390/ ijms20030625] [PMID] [PMCID].
14.
Mosaferi E, Majidi J, Mohammadian M, Babaloo Z, Monfaredan A, Baradaran B. HLA-G expression pattern: Reliable assessment for pregnancy outcome prediction. Adv Pharm Bull. 2013; 3(2):443-6. [DOI:10.5681/apb.2013.072] [PMID] [PMCID.
15.
LeMaoult J, Le Discorde M, Rouas-Freiss N, Moreau P, Menier C, McCluskey J, et al. Biology and functions of human leukocyteantigen-G in health and sickness. Tissue Antigens. 2003; 62(4):273-84. [DOI:10.1034/j.1399-0039.2003.00143.x] [PMID.
16.
Donadi EA, Castelli EC, Arnaiz-Villena A, Roger M, Rey D, Moreau P. Implications of the polymorphism of HLA-G on its function, regulation, evolution and disease association. Cell Mol Life Sci. 2011; 68(3):369-95. [DOI:10.1007/s00018-010-0580-7] [PMID] [PMCID].
17.
Berger DS. Molecular associations of recurrent spontaneous abortion [PhD. dissertation]. Pittsburgh, PA: University of Pittsburgh; 2007. http://d-scholarship.pitt.edu/10015/.
18.
Lau HH, Ng NHJ, Loo LSW, Jasmen JB, Teo AKK. The molecular functions of hepatocyte nuclear factors - In and beyond the liver. J Hepatol. 2018; 68(5):1033-48. [DOI:10.1016/j. jhep.2017.11.026] [PMID].
19.
Ober C, Aldrich CL, Chervoneva I, Billstrand C, Rahimov F, Gray HL, et al. Variation in the HLA-G promoter region influences miscarriage rates. Am J Hum Genet. 2003; 72(6):1425-35. [DOI:10.1086/375501] [PMID] [PMCID].
20.
Yazdani N, Shekari Khaniani M, Bastami M, Ghasemnejad T, Afkhami F, Mansoori Derakhshan S. HLA-G regulatory variants and haplotypes with susceptibility to recurrent pregnancy loss. Int J Immunogenet. 2018; 45(4):181-9. [DOI:10.1111/iji.12364] [PMID].
21.
Hviid TV, Rizzo R, Christiansen OB, Melchiorri L, Lindhard A, Baricordi OR. HLA-G and IL-10 in serum in relation to HLA-G genotype and polymorphisms. Immunogenetics. 2004; 56(3):13541. [DOI:10.1007/s00251-004-0673-2] [PMID].
22.
Dias FC, Bertol BC, Poras I, Souto BM, Mendes-Junior CT, Castelli EC, et al. The genetic diversity within the 1.4 kb HLAG 5 upstream regulatory region moderately impacts on cellular microenvironment responses. Sci Rep. 2018; 8(1):5652. [DOI:10.1038/s41598-018-24009-7] [PMID] [PMCID].
23.
Sipak O, Ry A, Grzywacz A, Laszczyska M, Zimny M, Karakiewicz B, et al. The relationship between the HLA-G polymorphism and sHLA-G levels in parental pairs with high-risk pregnancy. Int J Environ Res Public Health. 2019; 16(9):1546. [DOI:10.3390/ ijerph16091546] [PMID] [PMCID].
24.
Castelli EC, Ramalho J, Porto IO, Lima TH, Felcio LP, Sabbagh A, et al. Insights into HLA-G genetics provided by worldwide haplotype diversity. Front Immunol. 2014; 5:476. [DOI:10.3389/ fimmu.2014.00476] [PMID] [PMCID].
25.
Noci I, Fuzzi B, Rizzo R, Melchiorri L, Criscuoli L, Dabizzi S, et al. Embryonic soluble HLA-G as a marker of developmental potential in embryos. Human Reprod. 2005; 20(1):138-46. [DOI:10.1093/humrep/deh572] [PMID].
26.
Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 1988; 16(3):1215. [DOI:10.1093/nar/16.3.1215] [PMID] [PMCID].
27.
Tan Z, Shon AM, Ober C. Evidence of balancing selection at the HLA-G promoter region. Hum Mol Genet. 2005; 14(23):3619-28. [DOI:10.1093/hmg/ddi389] [PMID].
28.
Berger DS, Hogge WA, Barmada MM, Ferrell RE. Comprehensive analysis of HLA-G: Implications for recurrent spontaneous abortion. Reprod Sci. 2010; 17(4):331-8. [DOI:10.1177/1933719109356802] [PMID].
29.
Agrawal D, Prakash S, Misra MK, Phadke SR, Agrawal S. Implication of HLA-G 5 upstream regulatory region polymorphisms in idiopathic recurrent spontaneous abortions. Reprod Biomed Online. 2015; 30(1):82-91. [DOI:10.1016/j.rbmo.2014.09.015] [PMID].
30.
Quintero-Ronderos P, Laissue P. Genetic variants contributing to early recurrent pregnancy loss etiology identified by sequencing approaches. Reprod Sci. 2019; 1933719119831769. [DOI:10.1177/1933719119831769] [PMID].
31.
Wang X, Biernacka JM. Assessing the effects of multiple markers in genetic association studies. Front Genet. 2015; 6:66. [DOI:10.3389/fgene.2015.00066] [PMID] [PMCID].
32.
Karimzadeh Meybodi MA, Taheripanah R. Infections in Recurrent Miscarriage. J Reprod Infertil. 2000; 1(2):24-34. [In Persian] https://www.jri.ir/article/327##.

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