In Vitro Enzymatic Virulence Factors of Dermatophytes Species Isolated From Clinical Specimens

Faezeh Mohammadi; Amirhosein Gholamlou; Monirsadat Mirzadeh; Zeinab  Ghasemi; Hadi Aliyari

Journal of Inflammatory Diseases

The Official Journal of Qazvin University of Medical Sciences

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In Vitro Enzymatic Virulence Factors of Dermatophytes Species Isolated From Clinical Specimens

Author(s):

Faezeh Mohammadi^1,*,

Amirhosein Gholamlou²,

Monirsadat Mirzadeh

³,

Zeinab Ghasemi⁴,

Hadi Aliyari

1Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.

2Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran.

3Metabolic Disease Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.

4Department of Medical Mycology, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Journal of Inflammatory Diseases:Vol. 26, issue 1; 35-42

Article type:Research Article

How to Cite:Faezeh MohammadiAmirhosein GholamlouMonirsadat MirzadehZeinab GhasemiHadi AliyariIn Vitro Enzymatic Virulence Factors of Dermatophytes Species Isolated From Clinical Specimens.J Inflamm Dis.26(1):e156306.

Abstract

Background: Dermatophytes are keratinophilic fungi that affect the stratum corneum of the skin and keratinous structures. Violent factors play a vital role in the pathogenesis and antifungal resistance of dermatophytes. Objective: This study aims to evaluate the activity of extracellular enzymatic and biofilm formation as virulence factors of dermatophyte isolates. Methods: Fifty-eight dermatophyte isolates belonged to 27 Trichophyton. rubrum (46.6%), 19 T. mentagrophytes (32.8%), and 12 Microsporum. canis (20.7%) for evaluating the activity of phospholipase, hemolysin, proteinase, and biofilm formation were examined. The biofilm formed was analyzed by scanning electron microscopy (SEM). Findings: Evaluation of extracellular enzymes production revealed that 86.2%, 77.6 %, and 57% of dermatophyte strains were shown to be phospholipase, hemolysin, and proteinase producers, respectively. Furthermore, all isolates of T. rubrum and M. canis can produce phospholipase and hemolysin, respectively. There was a statistically significant difference between phospholipase activity and dermatophyte strains (P

Keywords

Dermatophytosis

Virulence factors

Biofilm

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