Abstract
Background:
Noise pollution causes of apoptosis in germinal cells of testes. This study investigated the effects of honey and vitamin E administration on the process of apoptosis in cells of the germinal testis of rats exposed to noise stress.Material and methods:
In this study, 24 male wistar rats randomly grouped as: 1 (honey + noise stress), 2 (vitamin E + noise stress) 3 (noise stress) and group 4 (control) groups. In groups 1, 2 and 3 rats were exposed to noise stress. After 50 days testes of rats were extracted and digested by using DNaseI, collagenase type IV and trypsin- EDTA then viability and apoptosis of the cells in harvested cell suspension evaluated by using the TUNEL kit.Results:
This study showed that the apoptosis was increased and viability decreased in testes of exposed rats to noise stress. By administrated of honey and vitamin E we observed that the apoptosis rate significantly decreased (1.89 % and 3.98% in groups received honey and vitamin respectively). (p<0.05).Conclusion:
This study showed that administration of honey and vitamin E reduced the effect of noise stress and apoptosis in germinal cell and recovered spermatogenesis in rats with increase numbers of live cells.Full Text
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Copyright
© 2013, Jentashapir Journal of Cellular and Molecular Biology. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.