https://doi.org/10.5812/jjm-120766

Molecular Characterization of Quinolone Resistance Determinants in Non-Typhoidal Salmonella Strains Isolated in Tehran, Iran

authors:

avatar Razie Helali-Pargali 1 , avatar Reza Ranjbar ORCID 1 , * , avatar Mojtaba Memariani ORCID 2 , avatar Ali Najafi 1 , avatar Caterina Mammina 3 , †

Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Sciences for Health Promotion and Mother–Child Care ‘G. D’Alessandro’, University of Palermo, Palermo, Italy
Corresponding Authors:
Jundishapur Journal of Microbiology: Vol.15, issue 5; e120766
published online: June 16, 2022
article type: Research Article
received: January 9, 2022
revised: April 26, 2022
accepted: May 8, 2022

how to cite: Helali-Pargali R, Ranjbar R, Memariani M, Najafi A , Mammina C . Molecular Characterization of Quinolone Resistance Determinants in Non-Typhoidal Salmonella Strains Isolated in Tehran, Iran. Jundishapur J Microbiol. 2022;15(5):e120766. https://doi.org/10.5812/jjm-120766.

Abstract

Background:

Quinolone resistant Salmonella serotypes have been reported in recent years and have become increasingly widespread worldwide.

Objectives:

We evaluated the molecular mechanism of quinolone resistance in non-typhoidal Salmonella strains isolated from clinical samples in Tehran, Iran.

Methods:

The present study included the Salmonella isolates originated from hospitalized individuals and outpatients in Tehran, Iran. Serotyping of nalidixic acid-resistant Salmonella isolates was done by slide agglutination method. Then, the quinolone resistance-determining region (QRDR) of topoisomerase gene gyrA and the plasmid-mediated quinolone resistance (PMQR) determinants were detected using the polymerase chain reaction (PCR) method. Restriction fragment length polymorphism (RFLP) analysis was also employed to determine the possible mutation in the gyrA gene of those strains. Mutant strains were detected by enzymatic digestion, and their PCR products were sequenced immediately.

Results:

Amongst 141 isolates, 60% showed nalidixic acid resistance, whereas none of them were ciprofloxacin-resistant. The commonly prevalent serotypes were S. Enteritidis and S. Infantis. Of 85 nalidixic acid-resistant strains, 17 (20%) isolates harbored the qnrS gene. However, PCR analysis of the quinolone-resistant strains did not detect qnrA and qnrB genes. PCR-RFLP and sequencing analysis of the QRDRs of the gyrA gene indicated that 16 (18.8%) isolates had mutant patterns, and the most common point mutation was serine to phenylalanine at position 83.

Conclusions:

Our results demonstrated that point mutations in gyrA and the existence of plasmid-mediated gene qnrS were important mechanisms of quinolone resistance in non-typhoidal Salmonella strains isolated from human origin. Other alternative mechanisms of resistance, such as alterations in the expression of efflux pumps, should be studied to provide greater insight into the molecular mechanism of quinolone-resistant non-typhoidal Salmonella isolates.

1. Background

Salmonella is one of the most important foodborne pathogens responsible for an approximately three billion cases of diarrheal diseases each year (1, 2). For non-typhoidal salmonellosis, the mean infective dose to produce symptomatic disease in healthy adults is 105 - 108. A smaller inoculum, on the other hand, can induce infections in newborns and people with specific underlying conditions like human immunodeficiency virus (HIV) infection, cancer, or other immune suppressing conditions (3, 4).

Water and animal-derived foods, such as meat, poultry, eggs, and dairy products, as well as raw fruits and vegetables, can cause non-typhoidal Salmonella infections (5, 6). In contrast to the invasive salmonellosis that requires prompt antibiotic therapy, antibiotics are not usually crucial for the treatment of Salmonella gastroenteritis. Trimethoprim/sulfamethoxazole (SXT), fluoroquinolones, and oxyimino-cephalosporins are commonly recommended as therapeutic options for patients with severe infections (7). Quinolone-resistant Salmonella isolates in people have been reported often in recent years and have become increasingly widespread worldwide (8, 9). Misappropriation of prescribed antibiotics and horizontal gene transfer have been responsible for the increase of Salmonella resistant to conventional antimicrobials (such as quinolones) in Iran (10, 11).

The main chromosomal point mutations occur in topoisomerase IV (parC and parE) and DNA gyrAse (gyrA and gyrB) encoding genes (10, 12). Meanwhile, quinolone resistance has been shown to be caused by changes in efflux pump production and DNA gyrAse protection by the qnr protein, which is created from plasmid-mediated quinolone resistance (PMQR) genes (qnrA, qnrB, qnrS, and aac(6′)Ib-cr) on a conjugative plasmid or transposon (8, 13). Quinolone resistance in foodborne Salmonella could be linked to the occurrence of foodborne outbreaks and the subsequent problem of clinical treatment. Thus, understanding the antibiotic resistance molecular mechanisms, especially quinolones, in Salmonella isolates can be helpful.

2. Objectives

The present study aimed to investigate the occurrence of quinolone resistance among non-typhoidal Salmonella strains isolated from patients in Tehran, Iran, and to elucidate the mechanisms behind it.

3. Methods

The present study involved Salmonella isolates originated from hospitalized individuals and outpatients in Tehran, Iran, based on laboratory confirmation and clinical presentations as described previously (7). A total of 141 Salmonella clinical strains were isolated, among which 113 (80%) strains were collected from stools, and 28 (20%) strains from blood, wound, urine, or other biological fluids. Only one isolate per patient was included in this study. Nalidixic acid-resistant Salmonella isolates were serotyped by slide agglutination method with anti-O and H antisera (Staten Serum Institute, Copenhagen, Denmark). Until use, the validated isolates were kept at -70°C in tryptic soy broth (TSB, Merck KGaA, Darmstadt, Germany) with 25% (v/v) glycerol (14). All ethical issues, including the subjects' health issues, dignity, integrity, right to self-determination, privacy, and confidentiality of personal information were considered.

According to the guidelines of the Clinical and Laboratory Standards Institute (CLSI), antimicrobial susceptibility to nalidixic acid (30 μg) and ciprfloxacin (5 μg) was performed on Mueller-Hinton agar (Oxoid Ltd.) using the Kirby-Bauer disk diffusion method (15). As reported previously, the method for DNA extraction was boiling. To amplify the quinolone resistance-determining region (QRDR) of topoisomerase genes gyrA, qnrS, qnrA, and qnrB among the nalidixic acid-resistant strains, the primer sets listed in Table 1 were used. Polymerase chain reaction (PCR) was performed in a volume of 25 µL using a thermal cycler (Eppendorf, Hamburg, Germany) for 30 cycles, as previously described. The cycling conditions were as follows: an initial denaturation at 94°C for 10 minutes followed by denaturation at 94°C for 1 minute, primer annealing at 52 - 60°C for 1 minute, primer extension at 72°C for 1 minute, and the final extension at 72°C for 7 minutes. The PCR products were separated by electrophoresis at 100V for 2 hours on 1.5% (w/v) agarose gels, and visualized using an ultraviolet (UV) transilluminator (Tanon, Shanghai, China).

Table 1.

PCR Primers Used in This Study

GenePrimerPrimer Sequence (5'→3')Amplicon Size (bp)Annealing Temperature (°C)Reference
gyrADsgyrAF: GAC GGA TCT CCG TAT AAC31752This study
R: TCT GGA TTA TGC GAT CTC
gyrALsgyrAF: TGT CCG AGA TGG CCT GAA GC34760(16)
R: TAC CGT CAT AGT TAT CCA CG
qnrADsqnrAF: ATT TCT CAC GCC AGG ATT TG51655(17)
R: GAT CGG CAA AGG TTA GGT CA
qnrBDsqnrBF: GTT GGC GAA AAA ATT GAC AGA A50052(18)
R: ACT CCG AAT TGG TCA GAT CG
qnrSDsqnrSF: ACG ACA TTC GTC AAC TGC AA41755(18)
R: TTA ATT GGC ACC CTG TAG GC

Restriction fragment length polymorphism (RFLP) was performed to determine the likely mutation in the gyrA gene among nalidixic acid-resistant strains. Briefly, 12 µL of PCR-amplified fragment was digested with 1 µL of HinfI (MBI Fermentas) in 3 µL digestion buffer in a final volume of 20 µL, and incubated at 37°C. After overnight incubation at 37°C, restriction fragments were subjected to 2% agarose gel electrophoresis, stained with ethidium bromide, and examined by UV transillumination before being photographed. To identify mutant isolates, PCR products were sequenced immediately (Pishgam co., Tehran, Iran). The DNA sequences of the mutant strains were deposited into the GenBank database (ncbi.nlm.nih.gov) under accession numbers KF975705, KF975707, KF975709, KF975710, and KF975711. As shown in Figure 1, nucleotide sequences obtained from sequencing analysis were translated into amino acid sequences and aligned by the Multiple Alignment using Fast Fourier Transform (MAFFT) program (mafft.cbrc.jp).

4. Results

Amongst the 141 Salmonella isolates, 60% were nalidixic acid-resistant strains. However, none of them were resistant to ciprofloxacin or showed reduced susceptibility to it. Also, a total of seven different serotypes were identified. The commonly prevalent serotypes were S. Enteritidis and S. Infantis (Table 2). According to our results, 17 (20%) of the 85 nalidixic acid-resistant strains carried the qnrS gene, all of which belonged to the Enteritidis serotype. Furthermore, the qnrA and qnrB genes were not found in nalidixic acid-resistant strains.

Table 2.

Distribution of Serotypes Among Nalidixic Acid-Resistant Salmonella Isolates

Salmonella SerotypeNalidixic Acid Resistant Isolates, No. (%)
Enteritidis39 (45.88)
Infantis35 (41.18)
Albany4 (4.71)
Hadar2 (2.35)
Munchen2 (2.35)
Typhimurium2 (2.35)
Haifa1 (1.17)
Total85 (100)

Non-mutant and mutant PCR-amplified gyrA fragments have three and two HinfI restriction sites, respectively (Table 3). Restriction analysis of the PCR product revealed that 16 (18.8%) isolates showed mutant pattern. Sequencing of gyrA PCR products was used to confirm mutations associated with nalidixic acid resistance in 16 resistant organisms that displayed two bands in PCR-RFLP. Nucleotide sequencing showed that 16 isolates had mutations in gyrA in terms of change of the amino acid serine to phenylalanine (Ser83→Phe, 7 isolates), serine to tyrosine (Ser83→Tyr, 5 isolates), aspartate to tyrosine (Asp87→Tyr, 3 isolates), and proline to leucine (Pro43→Leu, 1 isolate) (Figure 1). Amongst the seven isolates containing the substitution Ser83→Phe in gyrA, one isolate had multiple mutations (Ala51→Thr, Ser83→Phe, Val85→Leu, Ile89→Leu, Val90→Asp, Ala93→Ser, Gln94→His, Gly110→Arg, Ala117→Thr, and Ala118→Pro). Furthermore, two isolates harbored mutation at position 83 (Ser83→Phe) and carried qnrS gene (GenBank Accession Numbers: KF975711.1, KF975710.1, KF975709.1, KF975708.1, KF975707.1, KF975706.1, KF975705.1, KF975704.1, KC470542.1).

Alignment of the gyrA QRDR amino acid sequences of five Salmonella enterica subspecies enterica serotype Enteritidis with analogous sequence from reference strain of Salmonella Typhimurium_NCTC74.
Alignment of the gyrA QRDR amino acid sequences of five Salmonella enterica subspecies enterica serotype Enteritidis with analogous sequence from reference strain of Salmonella Typhimurium_NCTC74.
Figure 1.

Alignment of the gyrA QRDR amino acid sequences of five Salmonella enterica subspecies enterica serotype Enteritidis with analogous sequence from reference strain of Salmonella Typhimurium_NCTC74.

Table 3.

HinfI Restriction Pattern and the Length of Resulting Fragments

Gene and PrimerAmplicon Size (bp)Position of Restriction SitesRestriction Fragment Length (bp)
Non-mutantMutant
gyrA/ LsgyrA347108 and 207108, 99, and 140108 and 239 or 207 and 140
gyrA/ DsgyrA31733 and 13233,99 and 18533 and 284 or 132 and 185

5. Discussion

Salmonella infections are common in many developing nations, including Iran. They rarely appear as a severe public health hazard in the industrialized countries. In previous investigations conducted in Iran, S. enterica serotype Enteritidis was found to be the most common serotype of Salmonella in people. Moreover, in a comprehensive study conducted in China, United States, and Taiwan, S. enterica serotype Enteritidis was the most prevalent strain serotype among human isolates (19-22). These results are in agreement with our results, which revealed that S. enterica serotype Enteritidis was the predominant serotype. However, other studies in Ghana, Armenia, and Georgia reported S. enterica serotype Typhimurium as the main serotype (14, 23). In humans, S. Enteritidis infections are most usually linked to contaminated chicken and its products, whereas S. Typhimurium infections are mostly linked to infected pig and bovine derivatives (2). Local agriculture and farming practices, food distribution and consumption patterns, as well as food consumer preferences and behaviors could play a role in Salmonella serotype ranking in a well-defined geographical area like Tehran, Iran.

Over the past decade, since these organisms have been linked to clinical failures of therapy and a considerable burden of hospitalization, the high incidence of antimicrobial-resistant Salmonella isolates has been a critical public health concern (24-26). Resistance to nalidixic acid, for example, is a concerning scenario because fluoroquinolones are the most often used antibiotics for the treatment of invasive salmonellosis in adults, and failure of therapy in individuals with nalidixic acid-resistant Salmonella infections has been observed (27). In Iran, resistance to nalidixic acid has been increased among non-typhoidal S. enterica isolates (27), as in our study, 60% of isolates were resistant to nalidixic acid. In a previous study in Iran, a high level of resistance to nalidixic acid among Salmonella strains has been reported (28). In this regard, a meta–analysis study investigated fluoroquinolone-resistant clinical strains in Iran. According to their results, the pooled prevalence of nalidixic acid-resistant isolates in Iran was 48.1%. Moreover, from 1983 to 2019, the nalidixic acid-resistance trend of Salmonella serotypes was increasing in Iran (11).

In contrast, our results regarding the resistance to nalidixic acid in human isolates of S. Enteritidis were higher than those of South East Asian countries, including Malaysia, Thailand, and Vietnam (27 - 38%) (29-31). In addition to nalidixic acid, no ciprfloxacin-resistant isolate was found in the present study. This finding is in line with the current situation in Tehran, where no ciprofloxacin-resistant isolates were detected among 174 S. enterica strains (32). However, a comprehensive analysis by Khademi et al. revealed that the pooled occurrence of ciprofloxacin-resistant Salmonella serotypes in clinical specimens was 2.9% in Iran (11). In Salmonella spp., point mutations at QRDR of DNA gyrAse are mainly the cause of quinolone resistance between amino acids 67 and 106, which can alter the binding site of the agents with DNA gyrAse (33). Single mutations in the gyrA gene have been discovered to be sufficient to provide substantial levels of resistance to quinolones like nalidixic acid in Salmonella spp. (34).

In the present study, genetic characterization of nalidixic acid resistance revealed that all mutant S. enterica isolates had point mutations at codons 83 or 87 of GyrA, except one isolate that had point mutation at position 43 (Pro43→Leu), which was not previously reported. Double substitutions at both positions 83 and 87 were not identified in this study. The most common point mutation of gyrA occurred at position 83 (Ser→Phe, 43.7%). In contrast to our finding, previous reports from Iran showed that the predominant substitution was at position 87 (Asp87→Asn) of GyrA (32, 35). In agreement with the current study, the highest prevalence of amino acid substitution in GyrA was Ser83→Phe (92.1%) among nalidixic acid-resistant S. enterica serotype Typhi strains, isolated from southern Vietnam (36). Indeed, these reports from different countries indicate that the presence of mutation at Ser83 may cause reduced susceptibility to ciprofloxacin, representing high level of nalidixic acid resistance (36).

Plasmid-borne resistance, such as PMQR in S. enterica, is a public health concern because it results in horizontal fluoroquinolone resistance transfer between strains (37). In this study, 20% of isolates harbored the qnrS gene. Moreover, PCR analysis of the quinolone-resistant strains did not detect qnrA or qnrB genes. These findings partly agree with previous studies conducted in Korea and United States (10, 38). There are few studies investigating the PMQR in non-typhoidal Salmonella isolates in Iran. However, a recent study by Saboohi et al. in Iran demonstrated that 25.8%, 1.17%, and 1.17% of Salmonella spp. harbored qnrA, qnrB, and qnrS genes, respectively (39). Contrary to our finding, Abbasi and Ghaznavi-Rad revealed that among the PMQR genes, qnrS, qnrA, and qnrB were positive in 60%, 40%, and 20% of the isolates, respectively (28).

5.1. Conclusions

This study provided an insight into the molecular mechanism of quinolone resistance in non-typhoidal Salmonella strains isolated from patients in Tehran, Iran. We cannot rule out the presence of mutations outside of the sequenced region, but our findings suggest that other possible mechanisms may play a role regarding the quinolone resistance in Salmonella isolates. These mechanisms could include parE mutations, changes in expression of eflux pumps, modifications of the outer membrane proteins or even novel mechanisms. Further studies are required to monitor the spread of non-typhoidal Salmonella involving QRDR and PMQR carriers and to determine other mechanisms of quinolone resistance.

Acknowledgements

References

  • 1.

    Ranjbar R, Salimkhani E, Sadeghifard N, Yazdi JZ, Morovvati S, Jonaidi N, et al. An outbreak of gastroenteritis of unknown origin in Tehran, July 2003. Pak J Biol Sci. 2007;10(7):1138-40. [PubMed ID: 19070067]. https://doi.org/10.3923/pjbs.2007.1138.1140.

  • 2.

    Ferrari RG, Rosario DKA, Cunha-Neto A, Mano SB, Figueiredo EES, Conte-Junior CA. Worldwide epidemiology of Salmonella serovars in animal-based foods: a meta-analysis. Appl Environ Microbiol. 2019;85(14). [PubMed ID: 31053586]. [PubMed Central ID: PMC6606869]. https://doi.org/10.1128/AEM.00591-19.

  • 3.

    Crump JA, Sjolund-Karlsson M, Gordon MA, Parry CM. Epidemiology, clinical presentation, laboratory diagnosis, antimicrobial resistance, and antimicrobial management of invasive Salmonella infections. Clin Microbiol Rev. 2015;28(4):901-37. [PubMed ID: 26180063]. [PubMed Central ID: PMC4503790]. https://doi.org/10.1128/CMR.00002-15.

  • 4.

    Bula-Rudas FJ, Rathore MH, Maraqa NF. Salmonella infections in childhood. Adv Pediatr. 2015;62(1):29-58. [PubMed ID: 26205108]. https://doi.org/10.1016/j.yapd.2015.04.005.

  • 5.

    Sodagari HR, Wang P, Robertson I, Habib I, Sahibzada S. Non-typhoidal Salmonella at the human-food-of-animal-origin interface in Australia. Animals (Basel). 2020;10(7). [PubMed ID: 32674371]. [PubMed Central ID: PMC7401514]. https://doi.org/10.3390/ani10071192.

  • 6.

    Halaji M, Shahidi S, Ataei B, Atapour A, Feizi A, Havaei SA. Molecular epidemiology of blaCTX-M gene-producing uropathogenic Escherichia coli among Iranian kidney transplant patients: clonal dissemination of CC131 and CC10. Ann Clin Microbiol Antimicrob. 2021;20(1):65. [PubMed ID: 34496873]. [PubMed Central ID: PMC8424993]. https://doi.org/10.1186/s12941-021-00470-7.

  • 7.

    Ranjbar R, Naghoni A. Increase in nalidixic acid resistance among Salmonella enterica isolates recovered from patients in Tehran, Iran. SMU Med J. 2015;2(1):54-60.

  • 8.

    Sadeghi A, Halaji M, Fayyazi A, Havaei SA. Characterization of plasmid-mediated quinolone resistance and serogroup distributions of uropathogenic Escherichia coli among iranian kidney transplant patients. Biomed Res Int. 2020;2020:2850183. [PubMed ID: 33195692]. [PubMed Central ID: PMC7641683]. https://doi.org/10.1155/2020/2850183.

  • 9.

    Cuypers WL, Jacobs J, Wong V, Klemm EJ, Deborggraeve S, Van Puyvelde S. Fluoroquinolone resistance in Salmonella: insights by whole-genome sequencing. Microb Genom. 2018;4(7). [PubMed ID: 29975627]. [PubMed Central ID: PMC6113872]. https://doi.org/10.1099/mgen.0.000195.

  • 10.

    Kim KY, Park JH, Kwak HS, Woo GJ. Characterization of the quinolone resistance mechanism in foodborne Salmonella isolates with high nalidixic acid resistance. Int J Food Microbiol. 2011;146(1):52-6. [PubMed ID: 21354645]. https://doi.org/10.1016/j.ijfoodmicro.2011.01.037.

  • 11.

    Khademi F, Vaez H, Ghanbari F, Arzanlou M, Mohammadshahi J, Sahebkar A. Prevalence of fluoroquinolone-resistant Salmonella serotypes in Iran: a meta-analysis. Pathog Glob Health. 2020;114(1):16-29. [PubMed ID: 32013798]. [PubMed Central ID: PMC7144288]. https://doi.org/10.1080/20477724.2020.1719701.

  • 12.

    Gopal M, Elumalai S, Arumugam S, Durairajpandian V, Kannan MA, Selvam E, et al. GyrA ser83 and ParC trp106 Mutations in Salmonella enterica Serovar Typhi Isolated from Typhoid Fever Patients in Tertiary Care Hospital. J Clin Diagn Res. 2016;10(7):DC14-8. [PubMed ID: 27630841]. [PubMed Central ID: PMC5020279]. https://doi.org/10.7860/JCDR/2016/17677.8153.

  • 13.

    Ranjbar R, Behnood V, Memariani H, Najafi A, Moghbeli M, Mammina C. Molecular characterisation of quinolone-resistant Shigella strains isolated in Tehran, Iran. J Glob Antimicrob Resist. 2016;5:26-30. [PubMed ID: 27436462]. https://doi.org/10.1016/j.jgar.2016.01.010.

  • 14.

    Sedrakyan AM, Ktsoyan ZA, Arakelova KA, Zakharyan MK, Hovhannisyan AI, Gevorgyan ZU, et al. Extended-spectrum β-Lactamases in human isolates of multidrug-resistant non-typhoidal Salmonella enterica. Front Microbiol. 2020;11:592223. [PubMed ID: 33414769]. [PubMed Central ID: PMC7783090]. https://doi.org/10.3389/fmicb.2020.592223.

  • 15.

    Clinical and Laboratory Standards Institute (CLSI). PerformancePerformance Standards for Antimicrobial Susceptibility Testing. 30th ed. CLSI Supplement M100. Wayne, Pennsylvania: Clinical and Laboratory Standards Institute; 2020.

  • 16.

    Griggs DJ, Gensberg K, Piddock LJ. Mutations in gyrA gene of quinolone-resistant Salmonella serotypes isolated from humans and animals. Antimicrob Agents Chemother. 1996;40(4):1009-13. [PubMed ID: 8849216]. [PubMed Central ID: PMC163248]. https://doi.org/10.1128/AAC.40.4.1009.

  • 17.

    Lunn AD, Fàbrega A, Sánchez-Céspedes J, Vila J. Prevalence of mechanisms decreasing quinolone-susceptibility among Salmonella spp. clinical isolates. Int Microbiol. 2010;13(1):15-20. https://doi.org/10.2436/20.1501.01.107.

  • 18.

    Karczmarczyk M, Martins M, McCusker M, Mattar S, Amaral L, Leonard N, et al. Characterization of antimicrobial resistance in Salmonella enterica food and animal isolates from Colombia: identification of a qnrB19-mediated quinolone resistance marker in two novel serovars. FEMS Microbiol Lett. 2010;313(1):10-9. [PubMed ID: 20883500]. https://doi.org/10.1111/j.1574-6968.2010.02119.x.

  • 19.

    Zhan Z, Xu X, Gu Z, Meng J, Wufuer X, Wang M, et al. Molecular epidemiology and antimicrobial resistance of invasive non-typhoidal Salmonella in China, 2007-2016. Infect Drug Resist. 2019;12:2885-97. [PubMed ID: 31571942]. [PubMed Central ID: PMC6750164]. https://doi.org/10.2147/IDR.S210961.

  • 20.

    Singh N, Li X, Beshearse E, Blanton JL, DeMent J, Havelaar AH. Molecular Epidemiology of Salmonellosis in Florida, USA, 2017-2018. Front Med (Lausanne). 2021;8:656827. [PubMed ID: 33968960]. [PubMed Central ID: PMC8100233]. https://doi.org/10.3389/fmed.2021.656827.

  • 21.

    Zeng X, Lv S, Qu C, Lan L, Tan D, Li X, et al. Serotypes, antibiotic resistance, and molecular characterization of non-typhoidal salmonella isolated from diarrheic patients in Guangxi Zhuang Autonomous Region, China, 2014–2017. Food Control. 2021;120. https://doi.org/10.1016/j.foodcont.2020.107478.

  • 22.

    Chang YJ, Chen YC, Chen NW, Hsu YJ, Chu HH, Chen CL, et al. Changing antimicrobial resistance and epidemiology of non-typhoidal Salmonella infection in Taiwanese children. Front Microbiol. 2021;12:648008. [PubMed ID: 33868207]. [PubMed Central ID: PMC8044818]. https://doi.org/10.3389/fmicb.2021.648008.

  • 23.

    Acheampong G, Owusu M, Owusu-Ofori A, Osei I, Sarpong N, Sylverken A, et al. Chromosomal and plasmid-mediated fluoroquinolone resistance in human Salmonella enterica infection in Ghana. BMC Infect Dis. 2019;19(1):898. [PubMed ID: 31660876]. [PubMed Central ID: PMC6819380]. https://doi.org/10.1186/s12879-019-4522-1.

  • 24.

    Lo HY, Lai FP, Yang YJ. Changes in epidemiology and antimicrobial susceptibility of nontyphoid Salmonella in children in southern Taiwan, 1997-2016. J Microbiol Immunol Infect. 2020;53(4):585-91. [PubMed ID: 30017562]. https://doi.org/10.1016/j.jmii.2018.06.004.

  • 25.

    V. T. Nair D, Venkitanarayanan K, Kollanoor Johny A. Antibiotic-resistant Salmonella in the food supply and the potential role of antibiotic alternatives for control. Foods. 2018;7(10). [PubMed ID: 30314348]. [PubMed Central ID: PMC6210005]. https://doi.org/10.3390/foods7100167.

  • 26.

    Pribul BR, Festivo ML, de Souza MM, Rodrigues Ddos P. Characterization of quinolone resistance in Salmonella spp. isolates from food products and human samples in Brazil. Braz J Microbiol. 2016;47(1):196-201. [PubMed ID: 26887245]. [PubMed Central ID: PMC4822777]. https://doi.org/10.1016/j.bjm.2015.04.001.

  • 27.

    Malehmir S, Ranjbar R, Harzandi N. The molecular study of antibiotic resistance to quinolones in Salmonella enterica strains isolated in Tehran, Iran. Open Microbiol J. 2017;11:189-94. [PubMed ID: 29151995]. [PubMed Central ID: PMC5678238]. https://doi.org/10.2174/1874285801711010189.

  • 28.

    Abbasi E, Ghaznavi-Rad E. Quinolone resistant Salmonella species isolated from pediatric patients with diarrhea in central Iran. BMC Gastroenterol. 2021;21. 140. [PubMed ID: 33784974]. [PubMed Central ID: PMC8010990]. https://doi.org/10.1186/s12876-021-01719-3.

  • 29.

    Tiong V, Thong KL, Yusof MY, Hanifah YA, Sam JI, Hassan H. Macrorestriction analysis and antimicrobial susceptibility profiling of Salmonella enterica at a University Teaching Hospital, Kuala Lumpur. Jpn J Infect Dis. 2010;63(5):317-22. [PubMed ID: 20858996].

  • 30.

    Vo AT, van Duijkeren E, Gaastra W, Fluit AC. Antimicrobial resistance, class 1 integrons, and genomic island 1 in Salmonella isolates from Vietnam. PLoS One. 2010;5(2). e9440. [PubMed ID: 20195474]. [PubMed Central ID: PMC2829082]. https://doi.org/10.1371/journal.pone.0009440.

  • 31.

    Wannaprasat W, Padungtod P, Chuanchuen R. Class 1 integrons and virulence genes in Salmonella enterica isolates from pork and humans. Int J Antimicrob Agents. 2011;37(5):457-61. [PubMed ID: 21295950]. https://doi.org/10.1016/j.ijantimicag.2010.12.001.

  • 32.

    Hamidian M, Tajbakhsh M, Tohidpour A, Rahbar M, Zali MR, Walther-Rasmussen J. Detection of novel gyrA mutations in nalidixic acid-resistant isolates of Salmonella enterica from patients with diarrhoea. Int J Antimicrob Agents. 2011;37(4):360-4. [PubMed ID: 21371866]. https://doi.org/10.1016/j.ijantimicag.2010.12.013.

  • 33.

    de Souza RB, Magnani M, Ferrari RG, Kottwitz LB, Sartori D, Tognim MC, et al. Detection of quinolone-resistance mutations in salmonella spp. Strains of epidemic and poultry origin. Braz J Microbiol. 2011;42(1):211-5. [PubMed ID: 24031623]. [PubMed Central ID: PMC3768919]. https://doi.org/10.1590/S1517-83822011000100026.

  • 34.

    Ryan MP, Dillon C, Adley CC. Nalidixic acid-resistant strains of Salmonella showing decreased susceptibility to fluoroquinolones in the midwestern region of the Republic of Ireland due to mutations in the gyrA gene. J Clin Microbiol. 2011;49(5):2077-9. [PubMed ID: 21389151]. [PubMed Central ID: PMC3122662]. https://doi.org/10.1128/JCM.02574-10.

  • 35.

    Rahmani M, Peighambari SM, Svendsen CA, Cavaco LM, Agerso Y, Hendriksen RS. Molecular clonality and antimicrobial resistance in Salmonella enterica serovars Enteritidis and Infantis from broilers in three Northern regions of Iran. BMC Vet Res. 2013;9:66. [PubMed ID: 23561048]. [PubMed Central ID: PMC3623788]. https://doi.org/10.1186/1746-6148-9-66.

  • 36.

    Chau TT, Campbell JI, Galindo CM, Van Minh Hoang N, Diep TS, Nga TT, et al. Antimicrobial drug resistance of Salmonella enterica serovar typhi in asia and molecular mechanism of reduced susceptibility to the fluoroquinolones. Antimicrob Agents Chemother. 2007;51(12):4315-23. [PubMed ID: 17908946]. [PubMed Central ID: PMC2167998]. https://doi.org/10.1128/AAC.00294-07.

  • 37.

    Van TT, Nguyen HN, Smooker PM, Coloe PJ. The antibiotic resistance characteristics of non-typhoidal Salmonella enterica isolated from food-producing animals, retail meat and humans in South East Asia. Int J Food Microbiol. 2012;154(3):98-106. [PubMed ID: 22265849]. https://doi.org/10.1016/j.ijfoodmicro.2011.12.032.

  • 38.

    Basbas C, Byrne BA, Chigerwe M, Escobar ED, Hodzic E, Pires AFA, et al. Detection of cephalosporin and fluoroquinolone resistance genes via novel multiplex qPCR in fecal Salmonella isolates from Northern Californian dairy cattle, 2002-2016. Front Microbiol. 2021;12:601924. [PubMed ID: 33658984]. [PubMed Central ID: PMC7917062]. https://doi.org/10.3389/fmicb.2021.601924.

  • 39.

    Saboohi R, Rajaei B, Rad NS, Razavi MR, Aghasadeghi MR, Moshiri A, et al. Molecular detection and association of qnrA, qnrB, qnrS and blaCMY resistance genes among clinical isolates of Salmonella spp. in Iran. Adv Microbiol. 2014;4(1). https://doi.org/10.4236/aim.2014.41010.