The Evaluation of 660, 810, and 940nm Laser Wavelengths on Nystatin-Resistant Candida albicans: An in-vitro Study

authors:

avatar Saeed Mohammadi 1 , avatar Reyhaneh Shoorgashti ORCID 1 , avatar Ensieh Lotfali ORCID 2 , avatar Simin Lesan ORCID 3 , * , avatar Hooman Ebrahimi ORCID 4

Dentist, Tehran, Iran
Department of Medical Parasitology and Mycology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Oral Medicine Department, Islamic Azad University of Medical Sciences, Dental branch, Tehran, Iran
Assistant Professor, Oral Medicine Department, Islamic Azad University of Medical Sciences, Dental branch, Tehran, Iran.
Jundishapur Journal of Microbiology: Vol.17, issue 7; e144680
published online: August 28, 2024
article type: Research Article
revised: August 04, 2024
DOI: https://doi.org/10.5812/jjm-144680

how to cite: Mohammadi S, Shoorgashti R, Lotfali E, Lesan S, Ebrahimi H. The Evaluation of 660, 810, and 940nm Laser Wavelengths on Nystatin-Resistant Candida albicans: An in-vitro Study. Jundishapur J Microbiol. 2024;17(7):e144680. https://doi.org/10.5812/jjm-144680.

Abstract

Background: Candida albicans (C. albicans) is the main pathogen responsible for oral candidiasis. Emerging resistance of fungi to antifungal agents led to the development of new fungicidal modalities. 
Objectives: This study aimed to analyze the effects of 660, 810, and 940nm laser wavelengths on nystatin-resistant C. albicans. 
Methods: In this in-vitro study, a standard strain of C. albicans and eight nystatin-resistant isolates were irradiated with 660, 810, and 940nm diode lasers for 40 and 80 seconds (s) and compared with nystatin. The colony numbers and nystatin susceptibility using minimum inhibitory concentration were evaluated. Data were analyzed with SPSS26. 
Results: All laser wavelengths significantly decrease the colony count of standard-strain C. albicans, with 810nm laser irradiation for 40s causing the maximum reduction in colony count (P810nm<0.001). Similarly, all laser wavelengths significantly reduced the colony count of nystatin-resistant isolates, and they were more effective than nystatin (unlike the standard strain) (P<0.05). The 40s of 810nm laser irradiation demonstrated the greatest effect on MIC among different laser groups. 
Conclusions: The 810nm laser for 40s was most effective in reducing nystatin-resistant C. albicans colony count, and it also eliminated resistance in all clinical isolates.