Abstract
Background: Trichosporon asahii is an important yeast-like fungal species that exists in the environment and can colonize some human organs. Glucuronoxylomannan of Trichosporon asahii may play an important role in its anti-host immune effect.
Objective: To evaluate the anti-host immune effect of glucuronoxylomannan of Trichosporon asahii, the effect of glucuronoxylomannan of Trichosporon asahii on the cell viability and adenosine triphosphate production of macrophages were investigated.
Methods: Glucuronoxylomannan was separated by ethanol and cetyltrimethylammonium bromide precipitation. Glucuronoxylomannan was then purified by the CIA-modified purification method. A CCK-8 kit was used to detect the effect of glucuronoxylomannan on macrophage cell viability. An adenosine triphosphate detection kit was used to detect the changes in the adenosine triphosphate contents of macrophages under the glucuronoxylomannan intervention.
Results: The glucuronoxylomannan of Trichosporon asahii was successfully purified by the CIA-modified purification method. Under the glucuronoxylomannan intervention, the cell viability of macrophages first increased and then decreased over time. Glucuronoxylomannan induced decreases in the ATP production capacity of monocyte macrophages.
Conclusion: Trichosporon asahii can affect macrophages through glucuronoxylomannan-induced changes in energy metabolism.
Keywords
adenosine triphosphate cell viability glucuronoxylomannan Macrophages Trichosporon asahii
Copyright
© 2024, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.