https://doi.org/10.5812/jjm.4941

A Population-Based Study of Bacterial Spectrum in Febrile Neutropenic Patients

authors:

avatar Mohsen Meidani 1 , avatar Ahmad Bagheri 1 , * , avatar Farzin Khorvash 1

Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, IR Iran
Jundishapur Journal of Microbiology: Vol.6, issue 2; 150-156
published online: March 2, 2013
article type: Research Article
received: March 21, 2012
revised: May 22, 2012
accepted: June 20, 2012

how to cite: Meidani M, Bagheri A, Khorvash F. A Population-Based Study of Bacterial Spectrum in Febrile Neutropenic Patients. Jundishapur J Microbiol. 2013;6(2): 150-156. https://doi.org/10.5812/jjm.4941.

Abstract

Background:

Because of great concerns about mortality and morbidity of infection in febrile neutropenic patients, the appropriate empirical antibiotic should be started immediately. Although there are established guidelines for the use of empirical therapy, local microbiological pattern and antibiotic susceptibility should be considered.

Objectives:

The current study aimed to identify the etiological pathogens in febrile neutropenic cancer patients in Isfahan, Iran.

Patients and Methods:

This single-centre population-based study was conducted on 81 febrile neutropenic patients referring to Sayed-Al-Shohada hospital, the only referral malignant care center in Isfahan, Iran. Demographic data, duration and kind of malignancy, duration from last chemotherapy, duration of fever, and also physical exam were recorded for each patient. Moreover, procalcitonin, CRP, ESR, white blood cells, hemoglobin, platelet, and absolute neutrophil count were measured. BACTEC and E-test were used for blood culturing and determining the antibiotic susceptibility.

Results:

Out of 81 participants, 28.4% had positive blood cultures which mostly consisted of Gram-positive microorganisms. In addition, Staphylococcus epidermidis was the most isolated Gram positive bacteria (39.1%). ESR, CRP, and procalcitonin were significantly higher in patients with positive blood culture. The risk of infection increased with raise in duration of hospital stay, catheterization, increased pulse rate, increased oral temperature, low level of oxygen saturation, decreased systolic blood pressure, and low absolute neutrophile count. However, in this case, no relationship was found among the patients’ age, diastolic blood pressure, respiratory rate, duration of diagnosis, duration from last chemotherapy, duration of fever, white blood cells, hemoglobin, platelet, and finally the type of malignancies.

Conclusions:

It was concluded that Gram-positive bacteria were more prevalent as a cause of infection in the patients with malignancy and the most common pathogen was S. epidermidis. Larger studies are needed to determine the bacterial susceptibility of this center.

1. Background

Patients with malignancies are predisposed to severe infections, particularly during treatment with chemotherapic agents. One of the most common consequences of chemotherapy is neutropenia defined as the absolute neutrophil count (ANC) ˂ 500 cell/ μL (1, 2). Also, secondary to chemotherapy, most cells of the human body undergo undesirable changes which cause disruptions of physical defense barriers such as skin and mucosa. Moreover, exposures to potentially pathogenic microorganisms increase because of the frequent use of folly and venous access catheters in these patients (1). These factors increase the risk of infection in cancerous patients.

Febrile neutropenia is defined as having oral temperature ≥ 38.3°C or ≥ 38°C for more than one hour when ANC is less than 500 cell/ μL or the reduction of ANC to 500 cell/ μL in the next 24 to 48 hours. It can be the first sign of bacterial infection which requires immediate consideration (1, 2). Also, reactions to drugs or blood products and tumor-associated fever can be the reason of fever. However, because of life-threatening nature of bacterial infection in neutropenic patients, immediate antibiotic therapy is crucial (3).

With the introduction of empirical, broad-spectrum antibiotics since about 30 years ago, the mortality and morbidity of such condition decreased significantly (4). Since then, algorithmic approach help more in managing these patients (5). Although the use of the combination of an anti-pseudomonal b-lactam and an aminoglycoside became a base for published guidelines in managing febrile neutropenia, such guidelines indicate that with the identification of causative pathogens, the antibiotic regimen can be changed. This strategy not only increases the efficacy of therapy, but also decreases the side effects and the cost. However, it is important to have a broad-spectrum coverage which prevents breakthrough bacteremia (6).

Since the causative bacteria are often anonymous, knowing the prevalence of them in these patients was essential to have an adequate antimicrobial therapy. In the 70`s, in monomicrobial bacteremia, these bacteria mainly consisted of Gram-negative ones while with the advent of Coagulase-negative staphylococci (CoNS), Viridans streptococci, and Enterococci, this pattern changed to Gram-positive organisms in the 80`s and 90`s(7-9). In a comparison of febrile neutropenic children with adults, it was demonstrated that 64.2% and 57.9% of isolates were Gram positive in children and adults, respectively (10). However, today, febrile neutropenic populations with predominance of Gram-negative bacteria also exist.

In a single- center study in Israel, Gram-negative bacteria were predominant and their prevalence increased throughout two decades in adult population while in pediatric febrile neutropenic patients an increase in Gram-positive bacteria was shown. In two other studies conducted in Malaysia and Pakistan the same trend was observed (2, 11). Dong-Gun Lee et al. (12) mentioned that as a general characteristic of Asia-Pacific region, Gram-negative microorganisms were the major pathogens of infection.

Differences in bacterial spectrum of different centers demonstrate the importance of knowing the etiological pathogens of each center for better management of febrile neutropenic patients. Additionally, with the growing incidence of antimicrobial resistance, knowledge about the pattern and antibiotic sensitivity of causative microorganisms of each region and center is vital. It should be mentioned that the type and severity of immunosuppression has a great effect on the interpretation of the results of laboratory diagnostic tests (13, 14).

2. Objectives

The current study aimed to evaluate the microbiological spectrum and susceptibility patterns of pathogens in febrile neutropenic patients over one year in a university hospital of Iran.

3. Patients and Methods

3.1. Setting

This single-center population-based study was conducted in the only referral university cancer hospital of Isfahan (Iran) from February 2010 to February 2012.

3.2. Patient Eligibility

Febrile neutropenia was defined as oral temperature ≥ 38.3°C or ≥ 38°C for more than one hour when ANC was less than 500 cell/ μl or the reduction of ANC to 500 cell/ μl in the following 24 to 48 hours. The patients were randomly selected amongthe Sayed-Al-Shohada hospital patients, a referral hospital for patients with malignancies. Febrile neutropenic patients who were above 14 years and had not received empirical antibiotic therapy more than 12 hours of their commencements in the study were included.

A total of 81 febrile neutropenic patients were enrolled. All the steps and procedures were explained to the participants and written informed consents were taken from them. This trial was conducted in accordance with the Declaration of Helsinki. Ethical approval was obtained from the Isfahan University of Medical Sciences Ethics Committee.

3.3. Clinical and Laboratory Evaluations

Subjects were interviewed by a physician and data including age, gender, underlying malignancy, diagnosis date, duration of current hospital stay, duration of fever before sending blood culture, duration of having urinary or venous catheter were collected.

A phlebotomist was trained to do the venipuncture and obtain blood cultures in a manner to avoid any contamination during the procedure. Blood samples were taken for hemoglobin, white blood cell count, platelet count, Absolute neutrophil count(ANC), C-reactive protein (CRP), Erythrocyte sedimentation rate (ESR), and procalcitonin (PCT) analyses. ESR was determined by Westergren method while serum CRP was measured by immunoturbidimetrice method (COBAS Integra C reactive protein (Latex) test, Roche Diagnostics, Basel, Switzerland). Also, PCT levels in serum samples were determined using enzyme-linked immunoassay (ELISA kit: VIDAS BRAHMS Procalcitonin, bioMérieux, Lyon, France). All tests were performed according to manufacturer’s instructions and recommendations.

3.4. Bacterial Isolates

For blood cultures, the BACTEC 9050 blood culture instruments (Bectone Dickinson, Baltimore, Md.,USA) was used. The identification of etiological pathogens was based on routine bacteriological procedures. Routinely, the bottles were incubated in BACTEC for seven days. During these days, if the system alerted for positive blood culture, 3 to 5 drops of blood culture samples were inoculated on blood and chocolates agar plus 5% sheep blood. Then, after 24h of incubation aerobically, the samples were stained by Gram’s method.

3.5. Susceptibility Testing

The E-test from AB Biodisk, (Solna, Sweden) was employed in this study. The choice of antibiotic for each pathogen and the Minimum Inhibitory Concentration (MIC) were based on Clinical and Laboratory Standards Institute (CLSI) guidelines. For most of the isolates PDM Antibiotic Sensitivity Medium II (AB Biodisk) was used while 5% horse blood was added to medium for streptococcal isolates. The Fastidious Anaerobe Agar (IDG, UK) was used for anaerobic bacteria and Mueller-Hinton agar II (Difco Laboratories, Detroit, USA) plus 4% NaCl was employed to determine the susceptibility of the staphylococcal isolates to oxacillin.

3.6. Statistical Analysis

SPSS software version 15 (SPSS® Inc.) was employed to analyze the data , using Chi Square test and independent sample t-test to evaluate the relationship between bacterial infection vs. qualitative and quantitative variables, respectively. Z was considered as 0.95 while the P value of ˂ 0.05 was defined significant for all testes.

4. Result

4.1. Study Population

Out of 81 participants, 23 (28.4%) of them had positive blood culture while 58 (71.6%) patients had isolated febrile episodes. 30 women and 51 men participated in this study which 20.0% and 33.3% of them had positive blood culture, respectively. No significant difference was indicated in this respect (P value = 0.1).

4.2. Clinical and Laboratory Evaluations

Table 1 demonstrates the frequency (percentage) of different underlying malignancies based on the result of patients’ blood culture. No significant differences were found between the bacterial infection and the underlying malignancy (P value > 0.05).

The characteristics of 81 febrile neutropenic patients based on the results of their blood culture are summarized in Table 2 .

The percentage of graded scale of CRP level were as follows: in negative blood culture group 31.03%, 32.75%, 22.41%, 13.79%, and 0% of them had 0, +1, +2, +3, and +4 level of serum CRP respectively, while in the same order, it was 0%, 0%, 26.08%, 56.52%, and 17.39% for positive blood culture group. The Mann- Whitney analysis demonstrated a significant difference between the level of CRP and positive blood culture (P value = 0.00).

Table 1

The Frequency (Percentage) of Underlying Malignancies Based on the Result of Patients’ Blood Culture.

Positive, No. (%) Negative, No. (%) Total, No. P value
AML 6 (27.3) 16 (72.7) 22 P > 0.05
ALL 11 (44.0) 14 (56.0) 25 P > 0.05
CML 1 (25.0) 3 (75.0) 4 P > 0.05
CLL 0 (0) 3 (100) 3 P > 0.05
HCL 1 (50.0) 1 (50.0) 2 P > 0.05
Breast cancer 1 (20.0) 4 (80.0) 5 P > 0.05
Ovarian cancer 0 (0) 2 (100) 2 P > 0.05
Lymphoma 1 (16.7) 5 (83.3) 6 P > 0.05
MM 1 (16.7) 5 (83.3) 6 P > 0.05
Unknown 0 (0) 4 (100) 4 P > 0.05
Total 22 57 79 P > 0.05
Table 2

Comparison of Characteristics of 81 Febrile Neutropenic Patients Based on the Result of Their Blood Culture.

Positive Blood Culture, Mean ± SD Negative Blood Culture, Mean ± SD P value
Age, y 43.3 ± 18.7 48.2 ± 17.2 0.25
SBP 111.5 ± 14.9 120.6 ± 18.5 0.03
DBP 71.3 ± 9.7 76.8 ± 15.0 0.10
PR 88.8 ± 12.4 84.4 ± 7.2 0.05
RR 21.1 ± 3.0 19.4 ± 4.1 0.07
Oral T 39.0 ± 0.5 38.7 ± 0.5 0.02
O2 Sat 87.6 ± 8.7 92.0 ± 3.6 0.00
Duration of diagnosis, mo 11.7 ± 20.6 17.6 ± 31.1 0.43
Duration from last chemotherapy, day 23.3 ± 33.6 27.8 ± 22.2 0.57
Duration of catheterization, day 25.9 ± 26.7 6.1 ± 7.3 0.00
Duration of fever, hour 39.3 ± 43.1 23.3 ± 24.2 0.49
Duration of hospital stay, day 14.1 ± 8.4 4.8 ± 5.5 0.00
WBC ×10 3 2161.0 ± 6971.0 3782.4 ± 5685.7 0.28
HB 8.5 ± 1.7 9.1 ± 1.5 0.11
PLT ×10 3 31826.1 ± 27996.1 60874.1 ± 83156.1 0.10
ANC×10 3 190.2 ± 230.6 367.4 ± 293.7 0.01
PCT 29.9 ± 14.1 2.9 ± 5.0 0.00
ESR 75.0 ± 24.0 49.2 ± 23.1 0.00

4.3. Microorganisms and Susceptibility to Antimicrobial Agents

A total of 23 microorganisms were isolated which 56.4% (13) of them were Gram-positive bacteria, 17.4% (4) were Gram-negative bacillus, 3% (2) was Fusobacterium necrophorum (anaerobic bacilli), 4.3% (2) was Mycoplasma pneumonia (atypical bacteria), and 17.4% (4) were fungi. The most frequently isolated bacteria were S. epidermis with 39.1% (7). Other Gram-positive bacteria were consisted of S. aureus with 4.3% (2), streptococcus viridians with 4.3% (2), and S. pneumonia with 7.8% (1). Gram-negative bacillus included two Pseudomonas aeruginosa and two Escherichia coli, while one of the fungi was Mucor Spp., one was Candida albicans, and two were Aspergillus fumigates. The antibiotic sensitivity and resistance pattern of the isolated bacteria is given in Table 3 .

Table 3

Sensitivity and resistance pattern of common used antibiotics against isolated pathogens

  StaphylococcusepidermidisStaphylococcus aureusStreptococcus viridiansStreptococcus PneumoniaGram negative bacilliFusobacterium necrophorum Mycoplasma pneumonia
Penicillin, No. (%)              
S 3/9 (33.3) 0/1 (0) 0/1 (0) 0/2 (0) - 0/1 (0) -
R 6/9 (66.6) 1/1 (100) 0/1 (0) 2/2 (100) - 0/1 (0) -
Oxacillin, No. (%)              
S 5/9 (55.5) 0/1 (0) - - - - -
R 3/9 (33.3) 1/1 (100) 0/1 (0) - - - -
Ceftazidim, No. (%)              
S 5/9 (55.5) 0/1 (0) 0/1 (0) 1/2 (50) 1/4 (25.0) - -
R 3/9 (33.3) 1/1 (100) 0/1 (0) ½ (50) ¼ (25.0) - -
Cefepime, No. (%)              
S 6/9 (66.6) 0/1 (0) 1/1 (100) 2/2 (100) 3/4(75.0) - -
R 2/9 (22.2) 1/1 (100) 0/1 (0) 0/2 (0) 0/4 (0) - -
Meropenem, No. (%)              
S 8/9 (88.8) 0/1 (0) 1/1 (100) 2/2 (100) 4/4 (100) 1/1(100) -
R 0/9 (0) 1/1 (100) 0/1 (0) 0/2 (0) 0/4 (0) 0/1 (0) -
Imipenem, No. (%)              
S 8/9 (88.8) 0/1 (0) 1/1 (100) 2/2 (100) 4/4 (100) 1/1(100) -
R 0/9 (0) 1/1 (100) 0/1 (0) 0/2 (0) 0/4 (0) 0/1 (0) -
Piperacillin tazobactam, No. (%)              
S 7/9 (77.7) 0/1 (0) 1/1 (100) - 3/4 (75.0) 1/1(100) -
R 2/9 (22.2) 1/1 (100) 0/1 (0) - 1/4 (25) 0/1 (0) -
Vancomycin, No. (%)              
S 9/9 (100) 1/1 (100) 1/1 (100) 2/2 (100) - - -
R 0/9 (0) 0/1 (0) 0/1 (0) 0/2 (0) - - -
Ciprofloxacin, No. (%)              
S 7/9 (77.7) 0/1 (0) 0/1 (0) 0/2 (0) 2/4 (50.0) - 1/1 (100)
R 1/9 (11.1) 1/1 (100) 1/1 (100) 0/2 (0) 0/4 (0) - 0/1 (0)
Azithromycine, No. (%)              
S - - - 0/2 (0) - - 1/1 (100)
R - - - 2/2 (100) - - 0/1 (0)
Metronidazole, No. (%)              
S - - - - - 1/1(100) -
R - - - - - 0/1 (0) -

Abbreviations: S, Sensitive; R, Resistance

5. Discussion

During the past decades, the microbiological pattern of infection in febrile neutropenic patients with malignancies has considerably changed. This change includes a shifting epidemiology from Gram-negative to Gram-positive microorganisms. Nowadays, the most common bacteria in these patients are Coagulase-negative staphylococci, Alpha-haemolyic streptococci, S. aureus (9, 15, 16). Moreover, because of great concerns about mortality and morbidity of infection in such patients, antibiotic therapy should be initiated as soon as possible. This situation seems challenging since the bacteriological tests take at least 3-4 days. Therefore, the knowledge of prevalent pathogens in each region is a crucial key to begin the appropriate empirical antibiotic therapy immediately (17).

The blood cultures of the patients under study mostly consisted of Gram-positive microorganisms while Gram-negative bacteria and fungi stood in second place simultaneously. Moreover, S. epidermidis was the most common isolated bacteria. In the 70`s, Gram-negative pathogens were responsible for 70% of blood stream infections while in mid-80`s Gram-positive ones began to predominate and in the late90`s , these pathogens account for nearly 70% of bacteremia (9, 10, 18-20).

In a study conducted from 1986 to 1993 in a single institution in Spain, a continuous rise in Gram-positive bacteremia was seen, although the incidence of Gram-negative bacteremia remained unchanged. No italic staphylococci and S. viridans were the most commonly isolated bacteria in this study (21). Moreover, in a multicentre study on febrile neutropenic patients in the USA Wisplinghoff et al. (19) reported the outweigh of Gram-positive microorganisms as a cause of 62% and 76% of blood stream infections in 1995 and 2000 respectively, while only 22% and 14% of all blood stream infections originated from Gram-negative ones at the same time (19).

Another report from a New Zealand hospital also showed the predominance of Gram-positive bacteria. In the current study, Gram-positive cocci accounted for 46% of isolates while only 35% of which were Gram-negative bacilli (6). Additionally, Castagnola et al. (22) reported that in their study 57% and 41% of bacteremia in febrile chemotherapy-induced neutropenia were caused by Gram-positive and negative organisms respectively while in a study in Belgium, the relative frequencies of Gram-positive, Gram-negative, and polymicrobial bacteremia in the same patients were 57%, 34%, and 10%, respectively (23).

This changing pattern has been explained by some factors. As one of the important factors, intensive courses of chemotherapy cause oro-intestinal mucosa damage predisposing the patients to infection with Gram-positive microorganisms. Also, it was claimed that during chemotherapy the use of prophylactic oral antibiotics like selective intestine decontamination results in diminution of intestinal Gram-negative flora. Another factor is the frequent use of central venous catheters which contribute to developing skin-derived Gram-positive infections (1). Hand washing, better isolation and generally better sanitation of patients in oncology wards were other reasons of this event (17). In addition, as one of the main reasons, the use of some antibiotic prophylaxis such as third-generation cephalosporins and fluoroquinolones which are more active against Gram negative bacteria may explain this trend of changing (24). However, not only there are populations with predominance of Gram-negative organisms such as developing countries, but also it is claimed that the reemergence of Gram-negative bacteria as a major cause of infection in such patients were seen very recently (25).

During a study period of 14 years from 1988–2001 in Sweden, no epidemiological shift from Gram-negative to positive-organisms was seen in patients with malignant blood disorders and the balance between these two kinds of microorganisms was unchanged over the study period. In addition, Coagulase negative staphylococci with 17% was the predominant isolated bacteria and E. coli with 16% stood in second place (26). Moreover, Cattaneo et al. (27) stated that their study was an evidence for an epidemiological shift from Gram-positive to negative-bacteria in patients with hematological malignancies while E. coli was the most frequent organism. Also, there are other reports of emergence of Gram-negative bacteria mainly consisted of P. aeruginosa and Enterobacteriaceae which stands for nearly 50% of all documented infections (23).

Moreover, while the current study results demonstrated that 28.4% of the febrile neutropenic patients had positive blood culture, only 2.6% of blood cultures of such patients were positive in the previous retrospective study in the same center (28). Such difference can be explained by the use of BACTEC blood culture system instead of conventional culture method in the present study. In this center because of the higher cost of BACTEC, the conventional culture method is used for routine bacteria isolation. As indicated, the BACTEC system was much more efficient to isolate bacteria especially in shorter time and also had a lower incidence of contamination compared with the other systems (29, 30).

Finally, the current study results showed that the presence of fever in neutropenic patients did not actually mean the infection. Therefore, risk assessment for infection in these patients is a crucial point which should be considered for prescription of empirical antibiotic. ESR, CRP, and procalcitonin were markers of infection in the febrile neutropenic patients under study. In addition, the risk of infection increased with raise in duration of hospital stay, catheterization, increased pulse rate, increased oral temperature, low level of oxygen saturation, decreased systolic blood pressure, and low absolute neutrophile count. However, in this case, no relationship was found with the patients’ age, diastolic blood pressure, respiratory rate, duration of diagnosis, duration from last chemotherapy, duration of fever, white blood cells, hemoglobin, platelet, and finally the type of malignancies.

This cross-sectional study on febrile neutropenic patients with malignancy was the first of its kind conducted in Isfahan, Iran. However, the small study population was the drawback of this study which resulted in limitation for interpretation of some results. Therefore, larger studies are needed to determine the pattern of antibiotic sensitivity and resistance of these pathogens.

It was concluded that Gram positive-bacteria were more prevalent as a cause of infection in the patients with malignancy and the most common pathogen was S. epidermidis. Larger studies are required to confirm these results and to determine antibiotic sensitivity and resistance patterns.

Acknowledgements

References

  • 1.

    Oude Nijhuis CS, Daenen SM, Vellenga E, van der Graaf WT, Gietema JA, Groen HJ, et al. Fever and neutropenia in cancer patients: the diagnostic role of cytokines in risk assessment strategies. Crit Rev Oncol Hematol. 2002;44(2):163-74. https://doi.org/10.1016/S1040-8428(01)00220-7.

  • 2.

    Rasool Hassan BA, Yusoff ZB, Othman SB. Fever/clinical signs and association with neutropenia in solid cancer patients--bacterial infection as the main cause. Asian Pac J Cancer Prev. 2010;11(5):1273-7. [PubMed ID: 21198276].

  • 3.

    von Lilienfeld-Toal M, Dietrich MP, Glasmacher A, Lehmann L, Breig P, Hahn C. Markers of bacteremia in febrile neutropenic patients with hematological malignancies: procalcitonin and IL-6 are more reliable than C-reactive protein. Eur J Clin Microbiol Infect Dis. 2004;23(7):539-44. [PubMed ID: 15221617].

  • 4.

    Schimpff S, Satterlee W, Young VM, Serpick A. Empiric therapy with carbenicillin and gentamicin for febrile patients with cancer and granulocytopenia. N Engl J Med. 1971;284(19):1061-5. [PubMed ID: 4994878]. https://doi.org/10.1056/NEJM197105132841904.

  • 5.

    Freifeld AG, Bow EJ, Sepkowitz KA, Boeckh MJ, Ito JI, Mullen CA. Clinical practice guideline for the use of antimicrobial agents in neutropenic patients with cancer: 2010 update by the Infectious Diseases Society of America. Clin Infect Dis. 2011;52(4):e56. [PubMed ID: 21258094].

  • 6.

    Ritchie S, Palmer S, Ellis-Pegler R. High-risk febrile neutropenia in Auckland 2003-2004: the influence of the microbiology laboratory on patient treatment and the use of pathogen-specific therapy. Intern Med J. 2007;37(1):26-31.

  • 7.

    Aquino VM, Pappo A, Buchanan GR, Tkaczewski I, Mustafa MM. The changing epidemiology of bacteremia in neutropenic children with cancer. Pediatr Infect Dis J. 1995;14(2):140-3. [PubMed ID: 7746697]. https://doi.org/10.1097/00006454-199502000-00011.

  • 8.

    Koll BS, Brown AE. The changing epidemiology of infections at cancer hospitals. Clin Infect Dis. 1993;17(Suppl 2):S322-8. [PubMed ID: 8274595]. https://doi.org/10.1093/clinids/17.Supplement_2.S322.

  • 9.

    Sigurdardottir K, Digranes A, Harthug S, Nesthus I, Tangen JM, Dybdahl B, et al. A multi-centre prospective study of febrile neutropenia in Norway: microbiological findings and antimicrobial susceptibility. Scand J Infect Dis. 2005;37(6-7):455-64. https://doi.org/10.1080/00365540510038497.

  • 10.

    Hann I, Viscoli C, Paesmans M, Gaya H, Glauser M. A comparison of outcome from febrile neutropenic episodes in children compared with adults: results from four EORTC studies. International Antimicrobial Therapy Cooperative Group (IATCG) of the European Organization for Research and Treatment of Cancer (EORTC). Br J Haematol. 1997;99(3):580-8. [PubMed ID: 9401070]. https://doi.org/10.1046/j.1365-2141.1997.4453255.x.

  • 11.

    Zahid KF, Hafeez H, Afzal A. Bacterial spectrum and susceptibility patterns of pathogens in adult febrile neutropenic patients: a comparison between two time periods. J Ayub Med Coll Abbottabad. 2009;21(4):146-9. [PubMed ID: 21067049].

  • 12.

    Lee DG, Kim SH, Kim SY, Kim CJ, Park WB, Song YG, et al. Evidence-based guidelines for empirical therapy of neutropenic fever in Korea. Korean J Intern Med. 2011;26(2):220-52. [PubMed ID: 21716917]. https://doi.org/10.3904/kjim.2011.26.2.220.

  • 13.

    Bille J. Laboratory diagnosis of infections in febrile neutropenic or immunocompromised patients. Int J Antimicrob Agents. 2000;16(2):87-9. https://doi.org/10.1016/S0924-8579(00)00211-9.

  • 14.

    Chen CY, Tang JL, Hsueh PR, Yao M, Huang SY, Chen YC, et al. Trends and antimicrobial resistance of pathogens causing bloodstream infections among febrile neutropenic adults with hematological malignancy. J Formos Med Assoc. 2004;103(7):526-32. [PubMed ID: 15318274].

  • 15.

    Butt T, Afzal RK, Ahmad RN, Salman M, Mahmood A, Anwar M. Bloodstream infections in febrile neutropenic patients: bacterial spectrum and antimicrobial susceptibility pattern. J Ayub Med Coll Abbottabad. 2004;16(1):18-22. [PubMed ID: 15125174].

  • 16.

    Durnaa B, Dzierzanowska D. [Infection in neutropenic cancer patients--etiology, microbiological diagnostics, treatment]. Wiad Lek. 2006;59(7-8):506-11. [PubMed ID: 17209349].

  • 17.

    Latif SH, Anwar A, Ahmad I. Bacterial pathogens responsible for blood stream infection (BSI) and pattern of drug resistance in a tertiary care hospital of Lahore. Biomedica. 2009;25:101-5.

  • 18.

    Krupova I, Kaiserova E, Foltinova A, Kovacicova G, Kiskova M, Krchnakova A, et al. Bacteremia and fungemia in pediatric versus adult cancer patients after chemotherapy: comparison of etiology, risk factors and outcome. J Chemother. 1998;10(3):236-42. [PubMed ID: 9669650].

  • 19.

    Wisplinghoff H, Seifert H, Wenzel RP, Edmond MB. Current trends in the epidemiology of nosocomial bloodstream infections in patients with hematological malignancies and solid neoplasms in hospitals in the United States. Clin Infect Dis. 2003;36(9):1103-10. [PubMed ID: 12715303]. https://doi.org/10.1086/374339.

  • 20.

    Zinner SH. Changing epidemiology of infections in patients with neutropenia and cancer: emphasis on gram-positive and resistant bacteria. Clin Infect Dis. 1999;29(3):490-4. [PubMed ID: 10530434]. https://doi.org/10.1086/598620.

  • 21.

    Gonzalez-Barca E, Fernandez-Sevilla A, Carratala J, Granena A, Gudiol F. Prospective study of 288 episodes of bacteremia in neutropenic cancer patients in a single institution. Eur J Clin Microbiol Infect Dis. 1996;15(4):291-6. [PubMed ID: 8781879]. https://doi.org/10.1007/BF01695660.

  • 22.

    Castagnola E, Fontana V, Caviglia I, Caruso S, Faraci M, Fioredda F, et al. A prospective study on the epidemiology of febrile episodes during chemotherapy-induced neutropenia in children with cancer or after hemopoietic stem cell transplantation. Clin Infect Dis. 2007;45(10):1296-304. [PubMed ID: 17968824]. https://doi.org/10.1086/522533.

  • 23.

    Klastersky J, Ameye L, Maertens J, Georgala A, Muanza F, Aoun M. Bacteraemia in febrile neutropenic cancer patients. Int J Antimicrob Agents. 2007;30(Suppl 1):S51-9. [PubMed ID: 17689933]. https://doi.org/10.1016/j.ijantimicag.2007.06.012.

  • 24.

    Viscoli C, Varnier O, Machetti M. Infections in patients with febrile neutropenia: epidemiology, microbiology, and risk stratification. Clin Infect Dis. 2005;40(Suppl 4:):S240-5. [PubMed ID: 15768329]. https://doi.org/10.1086/427329.

  • 25.

    Feld R. Bloodstream infections in cancer patients with febrile neutropenia. Int J Antimicrob Agents. 2008;32(Suppl 1):S30-3. [PubMed ID: 18778919]. https://doi.org/10.1016/j.ijantimicag.2008.06.017.

  • 26.

    Cherif H, Kronvall G, Bjorkholm M, Kalin M. Bacteraemia in hospitalised patients with malignant blood disorders: a retrospective study of causative agents and their resistance profiles during a 14-year period without antibacterial prophylaxis. Hematol J. 2003;4(6):420-6. [PubMed ID: 14671614]. https://doi.org/10.1038/sj.thj.6200334.

  • 27.

    Cattaneo C, Quaresmini G, Casari S, Capucci MA, Micheletti M, Borlenghi E, et al. Recent changes in bacterial epidemiology and the emergence of fluoroquinolone-resistant Escherichia coli among patients with haematological malignancies: results of a prospective study on 823 patients at a single institution. J Antimicrob Chemother. 2008;61(3):721-8. [PubMed ID: 18218645]. https://doi.org/10.1093/jac/dkm514.

  • 28.

    Meidani M, Rostami M, Moulaee S. Blood culture in neutropenic patients with Fever. Int J Prev Med. 2012;3(2):141-2. [PubMed ID: 22347613].

  • 29.

    Cetin E, Kaya S, Demirci M, Aridogan BC. Comparison of the BACTEC blood culture system versus conventional methods for culture of normally sterile body fluids. Adv Ther. 2007;24(6):1271-7. [PubMed ID: 18165209]. https://doi.org/10.1007/BF02877773.

  • 30.

    Yew OF, Cheong YM, Zaidatul AR, Thanaletchimy N. Comparative study of a non-radiometric BACTEC system and a conventional blood culture system in a clinical microbiology laboratory. Malays J Pathol. 1995;17(1):11-5. [PubMed ID: 8906999].