1. Background
Plasmodium vivax is a protozoan parasite, which is most widely distributed around the world and responsible for more than 80 million malaria cases per year (1-3). Although the P. falciparum has a higher rate of mortality, P. vivax also accounts for remarkable morbidity and economic determinants in endemic regions (4-7). Plasmodium vivax is the most prevalent cause of malaria in different regions of Iran (8). This parasite is sensitive to most of the anti-malarial drugs, such as Chloroquine (CQ), which is applied as the first line treatment in the majority of countries (9, 10). Although CQ resistance has been reported in other regions of the world (11), CQ is still an efficient drug for the treatment of P. vivax single infections in south and southeast of Iran. It has been shown that due to multiple infections, there are primary signs of decreased sensitivity of P. vivax to CQ in Sistan and Baluchestan province (12).
Nowadays, regarding the worldwide extent of CQ resistance to P. falciparum, a combination of sulfadoxine and Pyrimethamine (SP) is used as the first-line antimalarial therapy in some countries (9). It has been shown that there is a considerable increase of CQ and SP resistance in P. vivax (11, 13, 14). Therefore, according to the increase of drug resistance in P. vivax, elimination of this species is further complicated (15). The SP consumption for the P. vivax treatment is unusual in the majority of malarious areas, in spite of the fact that, P. vivax isolates are exposed to SP, owing to mixed infections (P. falciparum and P. vivax) (16, 17). There are a remarkable number of cases with mixed infections in endemic regions of Iran (17); therefore, SP consumption to treat P. falciparum leads to drug resistance in P. vivax cases in these regions (12, 18).
It has been shown that specific point mutations in dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) genes are correlated with SP resistance (16, 19). In this regard, studies have shown that mutations at pvdhfr residues 57, 58, 61, 117, and 173 were correlated with anti-folate resistance (16, 20). Moreover, more than 20 different pvdhfr alleles have been described (21). Previous studies have shown mutations of the pvdhfr gene in south and southeast of Iran (12, 16). However, there is a lack of complete data on pvdhfr genotypes among Iranian cases, especially in endemic provinces and neighboring countries.
2. Objectives
The aim of this study was to investigate pvdhfr mutations and haplotypes in Sistan and Baluchistan endemic province of Iran.
3. Methods
3.1. Ethics Statement
The protocol of this study was reviewed and approved by the ethical and research committee of Zahedan University of Medical Sciences (Code: 7216). All cases had filled informed consent forms. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 2000 Helsinki declaration and its later amendments or comparable ethical standards.
3.2. Study area, Sample Collection, and Polymerase Chain Reaction Amplification
The Sistan and Baluchistan province is one of the 31 provinces of Iran. It is located in the southeast of Iran, bordering Pakistan and Afghanistan with geographical coordinates between 29.4924°N and 60.8669°E (Figure 1). The capital is Zahedan and the most prominent climatologically phenomenon of the region is heated with long, hot and dry summers and short winters, whereas, in the coastal region, near the Oman Sea, the weather is warm with a high percentage of humidity. These conditions are suitable for the transmission of malaria in this area. Blood samples were obtained from a total of 78 symptomatic patients with P. vivax infection in Sistan and Baluchistan province between years 2013 and 2015. Five milliliters of venous blood was taken in EDTA tubes and stored at -20°C. Blood films that were ready in field laboratories were rechecked. DNA was extracted by Q1Amp® DNA blood kit (Qiagen, US), according to the manufactures’ instructions. Detection of point mutations at codons number 57, 58, 61, and 117 were performed as described previously (12, 16).
Map of Sistan and Baluchistan Province, Iran; Districts and Geographical Outlines Are Depicted
3.3. In Silico-RFLP, PCR Amplification, and RFLP for 57, 58, 61, and 117 Positions
Detection of enzymatic digestion sites and produced fragments were done using the CLC DNA workbench software (version 1.0.1). Fragments of the pvdhfr gene (X98123) were digested with XmnI, PvuII (611 bp), BsrI (611 bp), Tsp45I (232 bp), and Alu I (238 bp) enzymes (Fermentase, Lithuania). These enzymes recognize F57L/I, S117N/T, T61M, and S58R residues in the pvdhfr gene. In order to detect mutations at 57, 117, 58, and 61 codons, the Pvdhfr gene was amplified by primer sequences (12, 16) (Table 1). Thermal profile for the PCR amplification was as follow: 94°C for 7 minutes; 35 cycles at 94°C for 50 seconds, 66°C for 50 seconds, 72°C for 65 seconds and a final extension at 72°C for 7 minutes. In order to detect the mutation at codon F57L/I, 10 μL of PCR products (608 bp) were digested with 10 U XmnI enzyme (Fermentase, Lithuania). In the case of S58R, 10 μL of PCR products (238 bp) were digested with 10 U AluI enzyme (Fermentase, Lithuania) for 15 hours at 37˚C in a final volume of 20 μL. For the S117N/T, 10 μL of PCR products (608 bp) were digested with 10 U PvuII enzyme (Fermentase, Lithuania) for 5 hours at 37˚C, and if there was a mutation in this codon (lack of cutting with PvuII), 10 μL of the mutant Pvdhfr gene PCR product was digested with 10 U BsrI enzyme (Fermentase, Lithuania) for 15 hours at 65°C in a final volume of 20 μL. In order to detect the mutation at codon T61M, 10 μL of PCR products (232 bp) was digested with 10 U Tsp 451 enzyme (Fermentase, Lithuania) for 16 hours at 37°C in a final volume of 20 μL. The DNA fragments derived from PCR or RFLP were electrophoresed on 2% agarose gel (12, 16).
| Position | Name | Sequence |
|---|---|---|
| 57 and 117 | VDT-OF | 5’-ATGGAGGACCTTTCAGATGTATTT¬GACATT-3’ |
| VDT-NR | 5’-TCACACGGGTAGGCGCCGTT¬GATCCTCGTG-3’ | |
| 58 and 61 | VDF-NF58 | 5’-GACCTTTCAGATGTATTTGACATTTACGGC-3’ |
| VDF-NR58 | 5’-GGTACCTCTCCCTCTTCCACTT¬TAGCTTCT-3’ |
The Name and Sequences of the Primers for PCR
3.4. DNA Sequencing and Phylogenetic Analysis
The pvdhps gene was amplified by nested PCR, as described previously (16). Some of the nested-PCR products were sequenced directly without cloning. To enter the DNA sequence for all samples, for adjustment of the sequence and nucleotides match up with chromatography to determine the similarity of each sample with other samples, the software Sequencher Tm 4.1.1 software (Gene Codes Corporation) was used. Phylogenetic analysis was also used by the MEGA6 software.
4. Results
4.1. The Frequency of Pvdhfr Gene Mutation
A total of 78 cases from Sistan and Baluchistan Province were analyzed for SNP/haplotype and appearance of mutation in 57, 58, 61, and 117 codons of the Pvdhfr gene using the PCR-RFLP method (Figure 2). All the isolates were examined for SNP/haplotype and the existence of the mutation in F57L/I, S58R, T61M, and S117N/T positions of the pvdhfr gene was determined using the PCR-RFLP method. All the products (232 bp) were digested with Tsp 45I enzyme (T61 = 171 bp + 61 bp), indicating the lack of mutation in T61M in the endemic provinces isolates. In the case of F57L/I, 2 (2.5%) cases showed mutation; PCR products containing mutation were not digested by the XmnI enzyme, indicating F57L/I mutation (611 bp = 445 bp + 166 bp). Sequencing showed that the mutated cases were F57I (Figure 2 and Table 2). Ten (12.8%) of the cases had shown the mutation in 58 codons. The PCR products containing S58R mutation were digested to 2 bands (three bands indicating a lack of mutation) by the AluI enzyme (S58 = 173 bp + 40 bp + 25 bp and 58R = 213 bp + 25 bp) (Figure 2 and Table 2). The S117N/T mutation was identified in 35.8% (28 cases) of cases. The PCR products (611 bp), which were digested by PvuII enzyme, indicated the lack of mutation in this codon (S117 = 350 bp + 261 bp) (Figure 2 and Table 2). The PCR products containing the mutation at codon 117 (117 N/T) were digested using the BsrI enzyme, in which S117N mutation displayed 3 bands, including 117N = 290 bp + 253 bp + 68 bp, whereas, the S117T genotype was not digested (Figure 2 and Table 2).
Restriction Fragment Length Polymorphism Patterns of the dhfr Gene for Detection of Mutation at Codons 117, 57, 58, and 61 Among the Isolates from Sistan and Baluchestan Province of Iran
| Isolate | Codon | No. (%) | |||
|---|---|---|---|---|---|
| 78 | 57 | 58 | 61 | 117 | |
| F | R | T | T | 2 (2.5) | |
| F | S | T | S | 46 (58.9) | |
| F | S | T | N | 19 (24.3) | |
| I | S | T | S | 2 (2.5) | |
| F | R | T | N | 6 (7.6) | |
| F | R | T | S | 2 (2.5) | |
| F | S | T | T | 1 (1.2) | |
Distribution of Mutations in the dhfr Gene in P. vivax Isolates from Sistan and Baluchestan Province of Iran
4.2. Distribution of Pvdhfr Gene Haplotypes
Seven different haplotypes of the pvdhfr gene were detected. The most prevalent wild haplotype was FSTS. The haplotypes with a single point mutation in 57I, 58R, 117N, and 117T forming the ISTS, FRTS, FSTN, and FSTT haplotypes, respectively, were also identified in the province. Moreover, haplotypes with double mutations at positions FRTN and FRTT were identified among the studied cases (Table 3). Haplotypes with 3 and 4 point mutations were not identified from Sistan and Baluchistan province.
| F 57 I/L | S 58 R | T 61 M | S 117 N/T | No. (%), N = 78 |
|---|---|---|---|---|
| I | S | T | S | 2 (2.6) |
| F | S | T | T | 1 (1.3) |
| F | R | T | T | 2 (2.6) |
| F | R | T | S | 2 (2.6) |
| F | R | T | N | 6 (7.7) |
| F | S | T | N | 19 (24.3) |
| F | S | T | S | 46 (59) |
Frequency of pvdhfr Alleles Based on Variations in Codons 117, 57, 58, and 61 Among the Isolates from Sistan and Baluchestan Province of Iran
4.3. Sequence Analysis of pvdhfr Gene in 611 bp Fragment and Phylogenetic Analysis
Sequencing was performed by Bioneer company (Korea) and registered in GenBank (accession numbers: MF381738 to MF381741). The main haplotype sequence of these genes, including the ISTS (L-VDT-OF), FSTN (P-VDT-OF), (Q-VDT-OF) FRTN, and FRTT (N-VDT-OF) were compared and mutations were confirmed and directly analyzed using ABI PRISMTM 310 automated sequencer to determine pvdhfr variation (haplotype) (Figure 3). Iranian pvdhfr phylogenetic tree was obtained based on bootstrap neighbor-joining method (MEGA6) in comparison with the recorded previous Iranian cases in GenBank (Figure 4).
Sequence Alignment of pvdhfr Gene at Codon 57 (A), Codon 58 (B) and Codon 117 (C)
Bootstrap Neighbor-Joining Consensus Tree of Iranian pvdhfr Isolates Data Set by MEGA 6. Sequence of Zahedan Isolate is Shown in Clad of Recorded Previous Iranian Sequences
5. Discussion
The present study indicated that in P. vivax isolates from Sistan and Baluchistan province, the pvdhfr gene was exposed to drug pressure due to SP. In these Iranian endemic areas, CQ is still used for P. vivax treatment. Attempts to control malaria are significantly challenged due to antimalarial drug resistance in endemic regions. For this reason, there is a necessity for annual monitoring of antimalarial drug usefulness and drug resistance in endemic regions (22). In the south provinces of Iran, such as Sistan and Baluchistan, however, CQ still remains efficient against the P. vivax malaria disease. An in vivo study demonstrates that P. vivax clearance time is increased in Sistan and Baluchistan province (23). This process could be a primary indication of decreased sensitivity of the P. vivax to CQ. Although anti-folates were consumed for the P. falciparum treatment, insufficient data was available regarding the source and increase of this drug resistance in P. vivax, especially in endemic provinces of Iran.
It has been shown that investigation of allelic variation will be useful at flanking microsatellite loci of the pvdhfr gene for comprehension of the basis and extension of anti-folate resistance in P. vivax isolates (24). Zakeri et al. previously reported 5 different haplotypes of the pvdhfr gene (16). In this study, 7 different haplotypes were identified. In addition, new haplotypes of ISTS and FRTT were detected. According to previous studies, the most prevalent non-mutated haplotype was FSTS among Sistan and Baluchistan cases. The wild-type pvdhfr haplotype was present with a high percentage of P. vivax populations from this province. This was similar to reports from malaria endemic regions in Afghanistan (25) and Pakistan (26). In this study, the frequency of mutation in codon 58 (12.8%) and 117 (35.8%) was similar to the author’s previous study (12). In this regard, several studies have shown that the frequency of 117N mutation among the isolates collected from Azerbaijan (71%) and Pakistan (93.5%) (26, 27) was higher than that of the current study (32%).
The haplotypes with one point mutation in locations 58R, 117N, and 117T to form FRTS, FSTN, and FSTT were observed in this study, in which FSTN was the second frequent haplotype of P. vivax isolates in Iran (25). In comparison with the current survey, highly mutant alleles were reported from Thailand, Vanuatu, Indonesia, and Papua New Guinea. In line with previous studies, in this study haplotypes with double mutations at FRTN were also found in Sistan and Baluchistan isolates, which was almost similar to that of obtained data from malarial endemic areas, such as Afghanistan and Pakistan (16, 25). Moreover, the distribution frequency of these haplotypes was not much different from previous studies (12). The present study did not find triple and quadruple mutations in any of the P. vivax isolates. In this regard, triple mutant haplotype has been reported in Afghanistan, which could be a warning for the arrival of these haplotypes to Iran from neighboring countries (25). However, due to the development of multi-drug resistance in P. falciparum, quadruple mutant alleles of pvdhfr at codons 57, 58, 61, and 117 were predominated in clinical isolates from India, Myanmar, Indonesia (28-30), and Thailand (31).
It appears that in these countries, diversity in the frequency of mutant pvdhfr alleles is a sign of drug pressure by usage of anti-folates. It has been reported that the 58R/117N mutant had a lower affinity for Pyrimethamine than the wild type enzyme (32), so that with an increase in the number of mutations, resistance to Pyrimethamine increases. Studies have shown that patients, whose parasites carried the allele of 57L/58R/61M/117T, were resistant to SP treatment (28, 33). Molecular analysis of the pvdhfr gene in Indian isolates showed haplotypes from double to quadruple mutants. Therefore, it seems that these genotypes have entered Iran from India and Pakistan as gene flow of drug resistance, which may be due to human migration. With changing treatment pattern in Iran from 2005, the SP became the first treatment option. Therefore, regarding greater availability of SP in endemic areas, such as Sistan and Baluchestan province, where P. vivax is still sensitive to CQ, there is a risk of an altering pattern of resistance in both P. falciparum and P. vivax, due to SP drug pressure (20). Moreover, it has been suggested that in Iran, as an endemic area, other anti-folates, such as trimethoprim/sulfamethoxazole, which are usually used against bacterial infections, could enhance the drug pressure in malarious areas as well.
5.1. Conclusions
This study showed the presence of double pvdhfr mutations in Sistan and Baluchestan province isolates. It presented important information to monitor the SP resistance in this region. Moreover, rising SP drug pressure suggested that the antifolate treatment against the P. falciparum malaria should be under control because it could affect the emergence of pvdhfr mutations, which may lastly bring about a complete SP resistance in P. vivax species. Consequently, permanent surveillance of P. vivax molecular markers is necessitated to check the expansion of SP resistance.
