A study of rdxA gene deletion in metronidazole resistant and sensitive Helicobacter pylori isolates in Kerman, Iran

authors:

avatar Hamid Abdollahi 1 , * , avatar Mohammad Javad Zahedi 2 , avatar Sodief Darvish Moghadam 2 , avatar Mehdi Hayatbakhsh Abasi 2

Department of Medical Microbiology, Virology and Immunology, Kerman University of Medical Sciences, mohammad1318@yahoo.com, Iran
Department of Gastroenterology, Kerman University of Medical Sciences, Iran
Jundishapur Journal of Microbiology: Vol.4, issue 2
article type: Research Article
received: January 1, 2011
accepted: January 20, 2011

how to cite: Abdollahi H, Javad Zahedi M, Darvish Moghadam S, Hayatbakhsh Abasi M. A study of rdxA gene deletion in metronidazole resistant and sensitive Helicobacter pylori isolates in Kerman, Iran. Jundishapur J Microbiol. 2011;4(2): -. 

Abstract

Introduction and objective: Metronidazole (Mtz) resistance in Helicobacter pylori has been found to be associated with mutations in rdxA, a gene encoding an oxygen insensitive NADPH nitroreductase, and enhanced by mutations in frxA a gene encoding a NAD(P)H-flavin oxidoreductase. In this study, we examined the prevalence of rdxA deletion among local isolates of H. pylori.
Materials and methods: We tested 63 H. pylori isolates obtained from 191 patients referred to endoscopy unit of Afzalipour hospital in Kerman, Iran, during 2009 for their susceptibility to common anti-H. pylori antibiotics using a modified disk diffusion test. Then the metronidazole resistant and sensitive isolates were evaluated for deletion in rdxA gene by PCR methods and compared with each other.
Results: From 35 resistant H. pylori isolates in this study only 22.9% (8 isolates) detected with deletion rdxA gene. No rdxA deletion was detected in sensitive isolates.
Conclusion: According to rdxA deletion rates in this study, it seems that some other nitroreductases are involved in metronidazole activation or there are other metronidazole resistance mechanisms involved.

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