Nalidixic acid susceptibility status of Salmonella enterica serovar Typhi isolates from Kolkata, India

Shyamapada Mandal; Manisha DebMandal; Nishith Pal

Jundishapur Journal of Microbiology

Ahvaz Jundishapur University of Medical Sciences

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Nalidixic acid susceptibility status of Salmonella enterica serovar Typhi isolates from Kolkata, India

Author(s):

Shyamapada Mandal

^1,*,

Manisha DebMandal²,

Nishith Kumar Pal³

1Department of Microbiology, Bacteriology and Serology Unit, Calcutta School of Tropical Medicine, C. R. Avenue, samtropmed@gmail.com, India

2Department of Physiology and Biophysics, KPC Medical College and Hospital, 1F Raja S C Mallick Road, Kolkata-700 032, India

3Department of Microbiology, Bacteriology and Serology Unit, Calcutta School of Tropical Medicine, C. R. Avenue, India

Jundishapur Journal of Microbiology:Vol. 4, issue 1

Article type:Research Article

How to Cite:Shyamapada MandalManisha DebMandalNishith Kumar PalNalidixic acid susceptibility status of Salmonella enterica serovar Typhi isolates from Kolkata, India.Jundishapur J Microbiol.4(1):null-null.

Abstract

Introduction and objectives: Typhoid is endemic in India; multidrug resistance of Salmonella enterica serovar Typhi has been reported from Kolkata, India, but nalidixic acid (Nx) resistance has not been documented in this part of the world. The aim of this study was to determine the Nx susceptibility for S. enterica serovar Typhi isolates (1991-2001) associated with enteric fever in Kolkata, India.

Materials and methods: The clinical S. enterica serovar Typhi isolates (n=421) were subjected to Nx (the prototype quinolone, which is used for in vitro screening tests for fluoroquinolone resistance) susceptibility testing by disc diffusion and determination of minimum inhibitory concentration (MIC) values. The test results, from the two methods, were compared by scattergram analysis, and the sensitivity and specificity in determining Nx resistance by disc testing, with respect to MIC values, were calculated.

Results: The S. enterica serovar Typhi isolates were categorized into resistant, intermediate and susceptible to Nx by disc testing and MIC. The isolates showed year-wise increment of Nx MICs (0.5-256mg/ml) during 1991-2001. High sensitivity (100%) and specificity (92.96%) in determining Nx resistance by disc testing, compared to MIC values were obtained for the isolates.

Conclusion: Increasing trend of Nx resistance, as determined by two in vitro methods, among S. enterica serovar Typhi isolates was noticed; such Nx resistance may help predict decreased susceptibility to ciprofloxacin (an anti-typhoid fluoroquinolone) among emerging S. enterica serovar Typhi in our part of the globe.

Keywords

Salmonella enterica serovar Typhi

Disc diffusion

Minimum inhibitory concentration

Nalidixic acid resistance

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