Prevalence of adenoviruses 40 and 41 in children less than five years suffering from acute gastroenteritis hospitalized in Ahvaz Abuzar Hospital

Alireza Samarbaf-Zadeh; Roya Pirmoradi; Ahmad Shamsizadeh; Manoochehr Makvandi

Jundishapur Journal of Microbiology

Ahvaz Jundishapur University of Medical Sciences

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Prevalence of adenoviruses 40 and 41 in children less than five years suffering from acute gastroenteritis hospitalized in Ahvaz Abuzar Hospital

Author(s):

Alireza Samarbaf-Zadeh^1,*,

Roya Pirmoradi²,

Ahmad Shamsizadeh²,

Manoochehr Makvandi²

1Research Center of Infectious and Tropical Diseases, Department of Medical Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, alirezasamarbaf_78@hotmail.com, Iran

2Lab Section, Salamat Hospital, Iran

Jundishapur Journal of Microbiology:Vol. 3, issue 2; 48-52

Article type:Research Article

Received:Dec 01, 2009

Accepted:Feb 01, 2010

How to Cite:Alireza Samarbaf-ZadehRoya PirmoradiAhmad ShamsizadehManoochehr MakvandiPrevalence of adenoviruses 40 and 41 in children less than five years suffering from acute gastroenteritis hospitalized in Ahvaz Abuzar Hospital.Jundishapur J Microbiol.3(2):48-52.

Abstract

Introduction and objective: Gastroenteritis, the inflammation of stomach and intestine, is caused by a variety of microorganisms including viruses. Especially adenoviruses type 40 and 41 of group F adenoviruses are two etiologies of gastroenteritis in newborns and infants of less than five years old. The aim of this study was to determine the prevalence of ad40 and ad41 inducing gastroenteritis in children hospitalized in Ahvaz Abuzar Hospital, Iran, during October 2007-2008.

Materials and methods: Fecal samples collected from the patients were tested first by an ELISA kit, specific for adenovirus detection. All samples including positive specimens by ELISA method were subjected to two rounds of PCR test. A specific pair of primers for adenoviruses 40 and 41 was applied PCR method.

Results: Out of 280 fecal specimens collected from diarrheic children, 7(2.5%) were positive by ELISA test and 12 (4.3%) by PCR method. All of positive samples belonged to ad41.

Conclusion: From group F of adenoviruses, adenovirus 41 is the major etiology of gastroenteritis in Ahvaz area.

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