Genotyping and identification of mycobacteria by fingerprinting techniques

authors:

avatar Azar Dokht Khosravi 1 , * , avatar Sepideh Seghatoleslami 2

Department of Medical Microbiology, School of Medicine, and Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, khosraviaz@yahoo.com, Iran
Department of Medical Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Iran

how to cite: Khosravi A D, Seghatoleslami S. Genotyping and identification of mycobacteria by fingerprinting techniques. Jundishapur J Microbiol. 2009;2(3): 81-91. 

Abstract

Tuberculosis (TB) remains a worldwide healthcare concern and has been characterized as an epidemic by World Health Organization (WHO). In the last few decades, DNA fingerprint techniques have become available to study the transmission of TB and other mycobacterial infections. The main gold standard typing technique is restriction fragment length polymorphism, which is widely used for molecular epidemiology purposes. However, other recently introduced techniques such as spoligotyping and MIRU-VNTR are also widely used. This review briefly summarizes the recent achievements in TB epidemiology associated with the introduction of molecular typing methods for Mycobacterium tuberculosis complex and non-M. tuberculosis complex mycobacteria. Besides, in this article, the application of molecular methods in the study of epidemiological aspects and diagnosis of mycobacterial diseases are highlighted.

Full Text

Full text is available in PDF