Diagnostic value of hydatid cyst antigens using western blotting method

Behzad Haghpanah; Badrossadat Mosavat; Zahra Ghayour; Farzad Oreizi

Jundishapur Journal of Microbiology

Ahvaz Jundishapur University of Medical Sciences

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Diagnostic value of hydatid cyst antigens using western blotting method

Author(s):

Behzad Haghpanah^1,*,

Badrossadat Mosavat²,

Zahra Ghayour²,

Farzad Oreizi²

1Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, [email protected], Iran

2Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Iran

Jundishapur Journal of Microbiology:Vol. 3, issue 4; 175-185

Article type:Research Article

Received:Feb 01, 2010

Accepted:Jun 01, 2010

How to Cite:Behzad HaghpanahBadrossadat MosavatZahra GhayourFarzad OreiziDiagnostic value of hydatid cyst antigens using western blotting method.Jundishapur J Microbiol.3(4):175-185.

Abstract

Introduction and objective: Hydatidosis is one of the most important and commonly found parasitic zoonoses in both humans and different animals, which is caused by the cestode helminthes Echinococcus granulosus. The diagnosis of the disease is primarily based on imagery techniques. Thus, highly sensitive and reliable serologic methods are required to confirm the diagnosis. AntigenB (AgB) and protoscoleces antigen (PSC Ag) were purified as two specific parasitic antigens and then evaluated against sera from two groups of hydatidosis and non-hydatidosis (control) subjects using the western blotting method in order to identify the most sensitive and specific antigen.

Materials and methods: Sera samples were taken from 22 patients under operation for hydatid cyst. 16 patients were also included as control group. Cyst fluid and protoscoleces were extracted and partially purified in a protein A column. Using SDS-PAGE, subunits of the cyst fluid antigen, AgB, and PSC Ag were identified. Finally, the subunits were transferred from gel to nitrocellulose membrane in a western blot test and reacted with hydatid and control sera in order to assess their sensitivity and specifity.

Results: Three antigens were identified as the subunits of AgB while 10 antigens were identified as PSC Ag. The sensitivity and specifity of AgB subunits in the western blot test were 77% and 100%, respectively. None of the PSC Ag subunits had both high sensitivity and high specifity concurrently.

Conclusion: It has been shown by the western blot test that the AgB 8/12 and 16 KDa subunit components had high diagnostic sensitivity and specifity levels (81% and 100%, respectively) and that they could presumably assist the physician in his pre- and post-operation diagnosis of hydatid cysts.

Keywords

Protoscoleces antigen

Antigen B

Western blotting

Hydatid cyst

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