In vitro activity of six antifungal drugs against clinically important dermatophytes

Keyvan Pakshir; Leila Bahaedinie; Zahra Rezaei; Manuchehr Sodaifi; Kamiar Zomorodian

Jundishapur Journal of Microbiology

Ahvaz Jundishapur University of Medical Sciences

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In vitro activity of six antifungal drugs against clinically important dermatophytes

Author(s):

Keyvan Pakshir^1,*,

Leila Bahaedinie²,

Zahra Rezaei²,

Manuchehr Sodaifi²,

Kamiar Zomorodian²,

1Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, pakshirk@sums.ac.ir, Iran

2Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Iran

Jundishapur Journal of Microbiology:Vol. 2, issue 4; 158-163

Article type:Research Article

Received:Oct 01, 2009

Accepted:Nov 01, 2009

How to Cite:Keyvan PakshirLeila BahaedinieZahra RezaeiManuchehr SodaifiKamiar Zomorodian et al.In vitro activity of six antifungal drugs against clinically important dermatophytes.Jundishapur J Microbiol.2(4):158-163.

Abstract

Introduction and objective: Dermatophytosis is a common fungal disease which involves the keratinized tissue. Several antifungal agents can be used to manage these infections. Unfortunately, drug resistant can result in treatment failure. The disk diffusion in vitro assay is a simple method that can be used to evaluate antifungal susceptibility in dermatophytes. The main aim of this study was to evaluate the antifungal activity of six antifungal drugs against several fresh clinical dermatophyte Iranian isolates.

Materials and methods: Forty clinical dermatophytes were isolated from patients suspected of having active dermatophytosis. Paper disks containing terbinafine, griseofulvin, clotrimazole, miconazole, fluconazole and ketoconazole were used in the disk diffusion method to evaluate the in vitro activity of the antifungal agents by measuring the mean diameter of inhibition around the disks.

Results: The isolates belong to three genera and eight species as: Trichophyton mentagrophytes 13(32.5%), T. rubrum 8(20%), Epidermophyton floccosum 7(17.5%), T. violaceum 4(10%), Microsporum gypseum 3(7.5%), T. tonsurans 2(5%), T. verrucosum 2(5%), T. schoenleinii 1(2.5%), and an unknown dermatophyte 1(2.5%). No isolates were resistant to clotrimazole and miconazole.

Conclusion: This study revealed that clotrimazole, miconazole, terbinafine, and griseofulvin were the most ideal antifungal drugs for the treatment of dermatophytosis. Disk diffusion method is a simple and valuable method for the evaluation of antifungal susceptibility of dermatophytes.

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