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Background:
One disadvantage of expressing heterologous proteins in
Jundishapur Journal of Microbiology
Ahvaz Jundishapur University of Medical Sciences
Using Rice Flour for Purification of Maltose Binding Fusion Proteins Expressed in Escherichia coli
Author(s):
1Department of Pathobiology, School of Veterinary Medicine, Shahid Chamran University, Ahvaz, IR Iran
2Department of Pathobiology, School of Veterinary Medicine, University of Lorestan, Khorram Abad, IR Iran
Published online:Apr 30, 2013
Article type:Research Article
Received:Mar 17, 2012
Accepted:Jun 30, 2012
How to Cite:Azadeh NeissiMasoudreza Seyfi Abad ShapouriMasoud Ghorbanpoor NajafabadiAmin JaydaryUsing Rice Flour for Purification of Maltose Binding Fusion Proteins Expressed in Escherichia coli.Jundishapur J Microbiol.6(3):233-6.https://doi.org/10.5812/jjm.4926.
Abstract
Objectives:
The purpose of this study was to investigate the use of rice flour, a natural source of amylose, for purification of MBP fusion proteins.
Materials and Methods:
MBP and a fusion protein of MBP and avian influenza virus nucleoprotein (MBP-NP) were expressed in
Results:
The results indicated that MBP and MBP-NP, both were successfully purified by rice flour.
Conclusions:
Rice flour can be used for purification of MBP fusion proteins. Although the efficiency of purification by rice flour is less than amylose resin, however, the yield is sufficient to obtain a quantity of protein required for research purposes.
Copyright
© 2013, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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