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Isolation of Enteropathogenic Escherichia coli (EPEC) From Raw Milk in Kermanshah by Polymerase Chain Reaction (PCR)

Author(s):
Pantea MohammadiPantea Mohammadi1, Ramin AbiriRamin AbiriRamin Abiri ORCID2,*
1Medical biology Research Center, University of Medical Sciences, Kermanshah, IR Iran
2Department of Microbiology, Faculty of Medicine, University of Medical Sciences, Kermanshah, IR Iran


Jundishapur Journal of Microbiology:Vol. 6, issue 4; 5439
Published online:Jun 09, 2013
Article type:Research Article
Received:May 04, 2012
Accepted:Aug 07, 2012
How to Cite:Pantea MohammadiRamin AbiriIsolation of Enteropathogenic Escherichia coli (EPEC) From Raw Milk in Kermanshah by Polymerase Chain Reaction (PCR).Jundishapur J Microbiol.6(4):5439.https://doi.org/10.5812/jjm.5439.

Abstract

Background:

Infectious diarrhoeal diseases cause major problems throughout the world and are responsible for considerable morbidity and mortality. Enteropathogenic Escherichia coli (EPEC) may cause infantile diarrhoea among children in developing countries.

Objectives:

The aim of this study was to define the prevalence of EPEC strains in raw milk samples.

Materials and Methods:

Raw milk samples collected from various cow farms in Kermanshah, Iran, during the period of 22nd June to 22nd September 2009 and were examined for EPEC presence using PCR reactions targeting eaeA, and then stx1 and stx2.

Results:

Of the 206 samples, 17 (8.25%) were contaminated with E. coli eaeA positive and stx1 and stx2 negative (EPEC).

Conclusions:

Our results confirm that raw milk recovered in Kermanshah may be a source for gastrointestinal infections by EPEC and strict preventive measures should be adopted to decrease contamination of milk with EPEC and other bacteria originated from animals.

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