Comparison of E test and Disk Diffusion Test for Antibiotic Resistance Testing of Enterotoxigenic and Non- Enterotoxigenic Bacteroides fragilis Isolated From Stools

How to Cite:Mohammad Taghi AkhiReza GhotaslouMahnaz ShirinzadehTahereh PirzadehMohammad Naghavi BehzadComparison of E test and Disk Diffusion Test for Antibiotic Resistance Testing of Enterotoxigenic and Non- Enterotoxigenic Bacteroides fragilis Isolated From Stools.Jundishapur J Microbiol.6(5):null-null.https://doi.org/10.5812/jjm.9800.

Abstract

Background:

Enterotoxigenic Bacteroides fragilis (ETBF) are one of the most important anaerobic bacteria whichcause diarrhea in human beings. A bft gene is coded for enterotoxin production called fragilysin. Most of them have acquired resistance to antianaerobicbacteria agent like other facultative anaerobic bacteria. E test and different dilution methods are usually performed for antimicrobial susceptibility determination of B. fragilis.

Objectives:

The aims of this study are to recognize ETBF by PCR method and also to evaluate efficiency thedisk diffusion method (DDM) in comparison with the E tests for antimicrobial susceptibility of B. fragilis isolates.

Materials and Methods:

bft gene was detected among 157 B. fragilis isolated from patients and healthy individuals by PCR. Antimicrobial susceptibility of all isolates was determined by DDM and E tests methods.

Results:

Nineteen (12.1%) B. fragilis containing bft gene from diarrheic (n = 14) and nondiarrheic (n = 5) feces were detected among 157 B. fragilis isolates. The highest resistance for ciprofloxacin, cefotaxim, cefoxitin with DDM and E test were 100%, 60%, and 65% respectively, while the lowest resistance in two methods was obtained for imipenem, piperacillin / tazobactam, and metronidazol. 100% agreement for some of antibiotics such as imipenem (P ? 0.05), and no correlation for others were observed among the antimicrobial susceptibility results obtained by the two methods (P ? 0.05).

Conclusions:

The presence of bft gene in B. fragilis isolates wouldnot certainly result in diarrheaamong patients. There is not enough accordance between DDM and Swedish E tests for antimicrobial susceptibility of B. fragilis for some antibiotics, although in other cases a good agreement was observed.

Keywords

<italic>Bacteroides fragilis</italic>

Antibiotic Resistance

Disk Diffusion Antimicrobial Tests

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Author(s):

Mohammad Taghi Akhi:[PubMed][Scholar]
Reza Ghotaslou:[PubMed][Scholar]
Mahnaz Shirinzadeh:[PubMed][Scholar]
Tahereh Pirzadeh:[PubMed][Scholar]
Mohammad Naghavi Behzad:[PubMed][Scholar]