Oxidative Stress Induced by Permanent Cerebral Hypoperfusion Improved by Red Grape Seed Extract in Rats

Alireza Sarkaki; Maryam Rafiei Rad; Seyed Ebrahim Hossini; Fereshteh Motamedi; Yaghoob Farbood; Mohammad Taghi Mansouri; Bahareh Naqhizadeh

Jundishapur Journal of Physiology

The Official Journal of Ahvaz Jundishapur University of Medical Sciences

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Oxidative Stress Induced by Permanent Cerebral Hypoperfusion Improved by Red Grape Seed Extract in Rats

Author(s):

Alireza Sarkaki^{1, 2},

Maryam Rafiei Rad^3,*,

Seyed Ebrahim Hossini⁴,

Fereshteh Motamedi⁵,

Yaghoob Farbood¹,

Mohammad Taghi Mansouri^{6, 7},

Bahareh Naqhizadeh⁶

1Department of Physiology, Persian Gulf Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

2Medicinal Plants Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

3Department of Biology, Islamic Azad University, Izeh Branch, Izeh, Iran

4Department of Biology, Sciences & Researches Branch, Islamic Azad University, Fars, Iran

5Iranian Neurosciences Research Network and Neurosciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

6Department of Pharmacology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

7Neuroanesthesia Laboratory, Department of Anesthesiology, Emory University School of Medicine, Atlanta, GA, USA

Jundishapur Journal of Physiology:Vol. 1, issue 1; 29-35

Published online:Oct 20, 2018

Article type:Research Article

Received:Apr 17, 2018

Accepted:Sep 16, 2018

How to Cite:Alireza SarkakiMaryam Rafiei RadSeyed Ebrahim HossiniFereshteh MotamediYaghoob FarboodMohammad Taghi MansouriBahareh Naqhizadehet al.Oxidative Stress Induced by Permanent Cerebral Hypoperfusion Improved by Red Grape Seed Extract in Rats.Jundishapur J Physiol.1(1):e148267.

Abstract

Introduction: This study aimed to evaluate the effect of chronic oral administration of red grape seed extract (GSE) on brain oxidative stress induced after permanent bilateral common carotid arteries occlusion (BCCAO) in male adult rats.
Methods: Thirty-two adult male Wistar rats (220±20g). The rats were divided randomly into four groups of 8 in each: 1) Sham+Veh; 2) Sham+GSE; 3) Isch+Veh; 4) Isch+GSE. In order to make animal model of permanent cerebral hypoperfusion/ischemia, both carotid arteries were ligated upper and lower and cut bilaterally. The animals in the treatment groups received a dose of 100 mg/kg GSE daily, by oral gavage (PO) for 4 weeks. Malondialdehyde (MDA) levels, Glutathione peroxidase (GPx) activity, and total thiol (–SH) groups were measured in homogenate of cerebral hippocampus and cortex.
Results: MDA elevated significantly in rats’ hippocampus and cortex after chronic cerebral hypoperfusion/ischemia when compared with sham rats (P<0.05 and P<0.001 respectively). Biochemical examinations revealed that GSE reversed the increased level of brain tissue malondialdehyde (MDA), and attenuated decreased activity of glutathione peroxidase (GPx) in BCCAO rats.
Conclusion: These findings suggest that GSE exhibits therapeutic potential for oxidative stress induced by cerebral hypoperfusion/ischemia, which is most likely related, at least in part, to its antioxidative and free radical scavenging actions.

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