Evaluation of Immunogenicity of Alginate Encapsulated Human Wharton's Jelly-Derived Mesenchymal Stem Cells in the Peritoneal Cavity of Rat

Saeed Azandeh; Masood Moghimi; Afshin Amari; Ghasem Saki; Darioush Bijan Nejad

Jundishapur Journal of Physiology

The Official Journal of Ahvaz Jundishapur University of Medical Sciences

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Evaluation of Immunogenicity of Alginate Encapsulated Human Wharton's Jelly-Derived Mesenchymal Stem Cells in the Peritoneal Cavity of Rat

Author(s):

Saeed Azandeh^1,*,

Masood Moghimi²,

Afshin Amari³,

Ghasem Saki¹,

Darioush Bijan Nejad¹

1Cellular and Molecular Research Center (CMRC), Medical Basic Sciences Research Institute, Department of Anatomy, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Ahvaz, Iran

2Cellular and Molecular Research Center (CMRC), Medical Basic Sciences Research Institute, Department of Immunology, Faculty of Medicine, Ahvaz University of Medical Sciences, Ahvaz, Iran

3Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Ahvaz, Iran

Jundishapur Journal of Physiology:Vol. 2, issue 1; 6-15

Published online:May 22, 2021

Article type:Research Article

Received:Aug 16, 2020

Accepted:Sep 28, 2020

How to Cite:Azandeh S, Moghimi M, Amari A, Saki G, Bijan Nejad D, Evaluation of Immunogenicity of Alginate Encapsulated Human Wharton's Jelly-Derived Mesenchymal Stem Cells in the Peritoneal Cavity of Rat.Jundishapur J Physiol.2021;2(1):e148610.

Abstract

Background: Human Wharton's Jelly-derived Mesenchymal Stem Cells (hWJ-MSCs) have immunosuppressive and anti-inflammatory properties. As such,
they exhibit an attractive therapeutic option for autoimmune disorders. Microencapsulation provides adequate protection against immune destruction of
transplanted cells. We aimed to investigate the cellular and humoral immune responses in the host rats transplanted with alginate microencapsulated hWJMSCs
in their peritoneal cavities.
Methods: hWJ-MSCs were microencapsulated in alginate microspheres and were transplanted intraperitoneally to rats. After 30 days, for the
evaluation of rats' cellular and humoral immunity against hWJ-MSCs, the spleen mononuclear cells of rats were stained with CFSE and co-cultured
with hWJ-MSCs for 3 days. Then the proliferation of spleen cells against hWJ-MSC was evaluated by flow cytometry, and Interferon-gamma (IFN-γ)
secretion was measured by ELISA. For evaluating humoral immunity, the serum of different rat groups was incubated with hWJ-MSCs, and serummediated
cytotoxicity was measured by MTT assay and antibody binding by cell-based ELISA.
Results: There were no differences between the spleen mononuclear cells proliferation, secretion of IFN-γ, serum-mediated cytotoxicity, and anti-WJMSCs
antibodies in rats transplanted with encapsulated and non-encapsulated hWJ-MSCs. Encapsulated and non-encapsulated hWJ-MSCs did not
stimulate the immune system in rats at the same time.
Conclusion: We suggest using a 3D culture for proliferation and having a more favorable function in the transplantation of these hWJ-MSCs.

Keywords

Alginate

Immunogenicity

Human Wharton's jelly-derived mesenchymal stem cells

Microencapsulation

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