Inhibitory effects of ethanolic, methanolic and hexanolic extracts of propolis on the activity and structure of tyrosinase

Nematollah Gheibi; Mohammad Sofiabadi; Sharam Rastak; Negar Taherkhani; Zahra Mohammadi; Zahra Golpour

doi:10.22110/jkums.v20i3.2920

Journal of Kermanshah University of Medical Sciences

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https://doi.org/10.22110/jkums.v20i3.2920

Inhibitory effects of ethanolic, methanolic and hexanolic extracts of propolis on the activity and structure of tyrosinase

Author(s):

Nematollah Gheibi¹,

Mohammad Sofiabadi^2,*,

Sharam Rastak³,

Negar Taherkhani⁴,

Zahra Mohammadi⁵,

Zahra Golpour³

1Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

2Dept. of Physiology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran

3Dept. of Anesthesiology, School of Paramedical Sciences, Qazvin University of Medical Sciences, Qazvin, Iran

4Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran

5Student Research Committee, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran

Journal of Kermanshah University of Medical Sciences:Vol. 20, issue 3; e69690

Published online:Dec 19, 2016

Article type:Research Article

Received:Aug 06, 2016

Accepted:Dec 03, 2016

How to Cite:Nematollah GheibiMohammad SofiabadiSharam RastakNegar TaherkhaniZahra MohammadiZahra Golpouret al.Inhibitory effects of ethanolic, methanolic and hexanolic extracts of propolis on the activity and structure of tyrosinase.J Kermanshah Univ Med Sci.20(3):e69690.https://doi.org/10.22110/jkums.v20i3.2920.

Abstract

Introduction: Tyrosinase is a key enzyme in the biosynthesis of melanin, which plays a crucial role in determining mammal’s skin and hair color. In this experimental study, the inhibitory effect of different extracts of propolis were investigated on tyrosinase activity.

Methods: Tyrosinase activity was measured in the presence of ethanolic, methanolic and hexanolic extracts of propolis by using thermal denaturation (Catecholase and cresolase reaction). Also the tyrosinase stability was examined in the presence of the extracts with the chemical (urea) denaturation method. Data were analyzed with SPSS software using ANOVA and Tokey post hoc test.

Results: Tm was 56.1 °C for tyrosinase in the absence of the extract and 47.4, 53.4 and 40.2 °C in the presence of ethanolic, methanolic and hexanolic extracts of propolis, respectively. Also ΔG 25 °C values were obtained 16.03 in the absence of the extracts and 13.5 (p<0.05), 15.3 and 13.3 (p<0.05) kJ/mol, respectively in the presence of ethanolic, methanolic and hexanolic extracts of propolis. In chemical denaturation, Cm was obtained 3.11 in the absence of the extracts and 15.8 (p<0.01), 11.9 (p<0.05), and 12.5(p<0.05) mM, respectively in the presence of ethanolic, methanolic and hexanolic extracts of propolis.

Conclusion: Ethanolic, methanolic and hexanaloic extracts of propolis are appropriate inhibitors for the tyrosinase. These reduce the thermal and chemical tyrosinase stability.

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Author(s):

Nematollah Gheibi:[PubMed][Scholar]
Mohammad Sofiabadi:[PubMed][Scholar]
Sharam Rastak:[PubMed][Scholar]
Negar Taherkhani:[PubMed][Scholar]
Zahra Mohammadi:[PubMed][Scholar]
Zahra Golpour:[PubMed][Scholar]