The agreement rate of rose Bengal, modified rose Bengal and rapid Wright tests for detection of positive serum sample of Brucellosis

Ali  Gorgeen Karaji; Firouzeh  Abdi; Mansour  Rezaei

Journal of Kermanshah University of Medical Sciences

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The agreement rate of rose Bengal, modified rose Bengal and rapid Wright tests for detection of positive serum sample of Brucellosis

Author(s):

Ali Gorgeen Karaji^1,*,

Firouzeh Abdi²,

Mansour Rezaei³

1Dept. of Immonology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

2Central Labratory, Kermanshah University of Medical Sciences, Kermanshah, Iran

3Dept. of Biostatistics and Epidemiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Journal of Kermanshah University of Medical Sciences:Vol. 15, issue 1; e69718

Published online:May 19, 2011

Article type:Research Article

Received:Jan 29, 2010

Accepted:Jun 06, 2010

How to Cite:Ali Gorgeen KarajiFirouzeh AbdiMansour RezaeiThe agreement rate of rose Bengal, modified rose Bengal and rapid Wright tests for detection of positive serum sample of Brucellosis.J Kermanshah Univ Med Sci.15(1):e69718.

Abstract

Background: Rose Bengal is a common test for serologic diagnosis of brucellosis. The aim of this study was to evaluate the agreement rate between modified rose Bengal, rose Bengal and rapid Wright tests for detection of antibody positive serum samples.

Methods: Cross section study was designed. 941 serum sample of patients referred to the central medical laboratory of Kermanshah, were evaluated by rose Bengal, modified rose Bengal and rapid Wright, using convenient sampling method. Positive samples were confirmed and their titers were defined by Wright agglutination test.

Results: The number of positive serum samples with modified rose Bengal was two fold higher than conventional rose Bengal test (P<0.001), which included all positive samples with rose Bengal. On the other hand, although the number of positive diagnosed serum samples with modified rose Bengal were nearly two times more than Wright test (P<0.001), but it did not include all positive samples detected by Wright test. All three tests showed acceptable agreement rate (k≥0.583).

Conclusion: Results of this study confirmed that modified rose Bengal able to detect more positive serum sample than conventional rose Bengal and it would be substituted rose Bengal. However, the rapid Wright test should be used aside with modified rose Bengal, because it could not cover all positive samples detected by rapid Wright test.

Keywords

rose Bengal

modified rose Bengal

rapid Wright

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