Lack of association between Interleukin-12 gene polymorphism (rs568408 G/A) and susceptibility to chronic hepatitis B virus infection

authors:

avatar Seddigheh Heidarian 1 , avatar Farzaneh Sabahi 1 , * , avatar Seyed Reza Mohebbi 2 , ** , avatar Mohammad Reza Zali 3

Department of Virology, Tarbiat Modares University, Tehran, Iran
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Corresponding Authors:

how to cite: Heidarian S, Sabahi F, Mohebbi S R, Zali M R. Lack of association between Interleukin-12 gene polymorphism (rs568408 G/A) and susceptibility to chronic hepatitis B virus infection. J Kermanshah Univ Med Sci. 2016;20(1):e69747. doi: 10.22110/jkums.v20i1.2685.

Abstract

Introduction: Hepatitis B is a potentially life-threatening infection that causes acute infection and chronic hepatitis with progression to cirrhosis and hepatocellular carcinoma (HCC). Interleukin-12 (IL12) is responsible for activation of Th1 immune responses, leading to possible clearance of HBV infection from the host’s body. The host’s immune-genetic background plays an important role in the pathogenesis of infectious diseases. The aim of the present study was to investigate the association of interleukin 12A single nucleotide polymorphism (rs568408 G/A) with chronic HBV infection.
Methods: In this case-control study, 120 chronic HBV patients and 120 healthy controls were studied from 2013 to 2015. Genotype analysis was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.
Results: The genotype distribution of IL12 rs568408 G/A was not significantly different between the chronic HBV patients and healthy controls. The frequency rates of the IL12 gene polymorphism at position rs568408 included GG (64.2%), AG (33.3%), and AA (2.5%) in the HBV patients and GG (68.3%), AG (29.2%), and AA (2.5%) in healthy controls (p=0.728).
Conclusion: The results suggested no significant association between IL12A rs568408 G/A genotypes and chronic hepatitis B virus infection.

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