Cytotoxic effects and morphological changes of silver nanoparticles in CHO-K1 cells

Masoumeh Heshmati; Sepideh Arbabi Bidgoli; Samideh Khoei; Seyed Mahdi Rezayat; Kazem Parivar

doi:10.22110/jkums.v19i5.2425

Journal of Kermanshah University of Medical Sciences

Peer-reviewed Medical Quarterly

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https://doi.org/10.22110/jkums.v19i5.2425

Cytotoxic effects and morphological changes of silver nanoparticles in CHO-K1 cells

Author(s):

Masoumeh Heshmati¹,

Sepideh Arbabi Bidgoli^2,*,

Samideh Khoei³,

Seyed Mahdi Rezayat⁴,

Kazem Parivar ¹

1Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2Dept. of Toxicology and Pharmacology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran

3Medical Physics Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

4Dept. of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Journal of Kermanshah University of Medical Sciences:Vol. 19, issue 5; e69814

Published online:Nov 19, 2015

Article type:Research Article

Received:May 04, 2015

Accepted:Oct 06, 2015

How to Cite:Masoumeh HeshmatiSepideh Arbabi BidgoliSamideh KhoeiSeyed Mahdi RezayatKazem Parivar Cytotoxic effects and morphological changes of silver nanoparticles in CHO-K1 cells.J Kermanshah Univ Med Sci.19(5):e69814.https://doi.org/10.22110/jkums.v19i5.2425.

Abstract

Background: Silver nanoparticles are of interest to be used as antimicrobial agents in medical care, wound dressings and cosmetics. Despite the fact that AgNPs are among the most commercialized nano materials owing to their specific antimicrobial properties, there is limited information about their risk assessment and possible hazards to human health and environment. Thus, this study was carried out to evaluate the cytotoxicity and morphological changes of different concentrations of two samples of commercialized silver nanoparticles (A and B) in CHO-K1 cells using MTT assay.

Methods: The cytotoxicity effect of silver nanoparticles AgNP-A (19.6 nm in diameter) and AgNP-B (15 nm in diameter) on CHO-K1 was evaluated in the range of 0.005-500 µg/ml after 24 hours of treatment using MTT assay. The morphological changes of treated cells were examined by light microscopy.

Results: Based on the results of cytotoxicity by MTT assay, reduced viability of cells and cytotoxicity were observed. The 50% inhibitory concentration (IC50) of silver nanoparticles was recorded at 100 and 10 µg/ml for AgNP-A and AgNP-B, respectively. Moreover, microscopic observations indicated clear morphological changes of treated cells.

Conclusion: Concentration and physicochemical properties of silver nanoparticles like size, shape, surface area, coating and zeta potential play a role in cytotoxicity and morphological changes of treated cells.

Keywords

Silver nanoparticles

cytotoxicity

CHO-K1

physicochemical properties

MTT assay

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Author(s):

Masoumeh Heshmati:[PubMed][Scholar]
Sepideh Arbabi Bidgoli:[PubMed][Scholar]
Samideh Khoei:[PubMed][Scholar]
Seyed Mahdi Rezayat:[PubMed][Scholar]
Kazem Parivar :[PubMed][Scholar]