The effect of β-ionone on telomerase activity in the human leukemia cell line K562

Zohreh Faezizadeh; Amir Gharib; Masoud Goudarzi

doi:10.22110/jkums.v19i3.2160

Journal of Kermanshah University of Medical Sciences

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https://doi.org/10.22110/jkums.v19i3.2160

The effect of β-ionone on telomerase activity in the human leukemia cell line K562

Author(s):

Zohreh Faezizadeh^1,*,

Amir Gharib²,

Masoud Goudarzi³

1Department of Laboratory Sciences, Faculty of Paramedical Sciences, Borujerd Branch, Islamic Azad University, Borujerd

2Department of Laboratory Sciences, Faculty of Paramedical Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran

3Department of Biology, Faculty of Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran

Journal of Kermanshah University of Medical Sciences:Vol. 19, issue 3; e69871

Published online:Jul 19, 2015

Article type:Research Article

Received:Jan 10, 2015

Accepted:Jun 02, 2015

How to Cite:Zohreh FaezizadehAmir GharibMasoud GoudarziThe effect of β-ionone on telomerase activity in the human leukemia cell line K562.J Kermanshah Univ Med Sci.19(3):e69871.https://doi.org/10.22110/jkums.v19i3.2160.

Abstract

Background: Telomerase is highly activated in most human cancer cells, therefore, its inhibition has been proposed as a novel and promising strategy for cancer therapy. Many plant-derived anticancer agents act through inhibition of telomerase activity and induction of apoptosis. β-ionone, a carotenoid compound isolated from Roseaceae, has been reported to possess anticancer properties. The present study was undertaken to examine the mechanism of β-ionone-induced apoptosis in human leukemia cell line K562 with special emphasis on its role in telomerase inhibition.

Method: In this study the anti-proliferation effect of β-ionone on K562 cells was evaluated by MTT assay. Apoptosis rate was detected by Hoechst staining and flow cytometry analysis. Telomerase activity was measured by (TRAP) ELISA assay.

Results: Exposure of K562 cells to β-ionone caused a dose-dependent decrease in proliferation. Flow cytometry analysis and Hoechst staining showed that percentage of apoptotic cells markedly increased with an increase in β-ionone concentration. Compared to control cells, treatment of K562 cells with β-ionone resulted in a significant decrease of telomerase activity. Moreover, a positive correlation was detected between telomerase inhibition and apoptosis induction in the treated K562 cells.

Conclusion: Based on these results, β-ionone is an appropriate candidate for inhibiting telomerase activity in K562 cells. Therefore, it may be utilized as a novel drug against some leukemia cell lines.

Keywords

human leukemia

β-ionone

telomerase inhibitors

apoptosis

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Author(s):

Zohreh Faezizadeh:[PubMed][Scholar]
Amir Gharib:[PubMed][Scholar]
Masoud Goudarzi:[PubMed][Scholar]