The association between glutathione s-transfrases T1, M1, and P1 polymorphisms with end stage renal disease

Hamid Nomani; Ali Aidy; Asad Vaisi Raygani; Lida HaghNazari; Dariush Reissi; Hadi Mozafari

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The association between glutathione s-transfrases T1, M1, and P1 polymorphisms with end stage renal disease

Author(s):

Hamid Nomani¹,

Ali Aidy^1,*,

Asad Vaisi Raygani¹,

Lida HaghNazari¹,

Dariush Reissi²,

Hadi Mozafari¹

1Dept. of Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

2Kidney Transplantation Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Journal of Kermanshah University of Medical Sciences:Vol. 17, issue 8; e74390

Published online:Nov 29, 2013

Article type:Research Article

Received:Apr 13, 2013

Accepted:Sep 10, 2013

How to Cite:Hamid NomaniAli AidyAsad Vaisi RayganiLida HaghNazariDariush ReissiHadi Mozafariet al.The association between glutathione s-transfrases T1, M1, and P1 polymorphisms with end stage renal disease.J Kermanshah Univ Med Sci.17(8):e74390.

Abstract

Background: End stage renal disease or ESRD is a progressive and irreversible deterioration in renal function in which the body loses ability to maintain metabolical balance, fluid and electrolytes. Glutathione s-transfrases (GSTs) are members of multigenic family which have essential role as an antioxidant in the cells. In this study, the polymorphism of GST, T1, M1 & P in ESRD patients were compared with those of control groups to determine the possible relationship between polymorphism of these enzymes and ESRD.

Methods: 136 of ESRD patients as experimental and 137 of healthy individuals as control group were examined. The GST T1 & M1 genotypes were determined in all individuals with multiplex- PCR and GST P1 polymorphism was determined with PCR-RFLP.

Results: The distribution of GSTT1& M1 of T1⁺-M1⁺(wild), T1⁺-M1¯, T1¯-M1⁺and T1¯-M1¯(null) genotypes in control group were 45(32.8%), 40(29.2%), 28(20.4%) and 24(17.5%) and in experimental group were17(12.8%), 46(34.6%), 21(15.8%) and 49(36.8%) respectively (P<0.001).

Conclusion: As the reduction of GST T1⁺-M1⁺ (wild) was observed with more frequency in patients with ESRD compared to control group (12.8%vs.32.8%), this genotype can be associated with the risk of ESRD.

Keywords

GST

ESRD

PCR-RFLP

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