Growth inhibition and Induction of apoptosis in K562 Chronic Myeloid Leukaemia cells by the derivatives of dihydropyrano[c]chromenes family

Sina Pejman; Majid Mahdavi; Saeed Balalaei

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Growth inhibition and Induction of apoptosis in K562 Chronic Myeloid Leukaemia cells by the derivatives of dihydropyrano[c]chromenes family

Author(s):

Sina Pejman¹,

Majid Mahdavi^1,*,

Saeed Balalaei²

1Dept. of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran

2Dept. of Organic Chemistry, K. N. Toosi University of Tech, Tehran, Iran

Journal of Kermanshah University of Medical Sciences:Vol. 17, issue 7; e74458

Published online:Oct 29, 2013

Article type:Research Article

Received:Apr 08, 2013

Accepted:Aug 03, 2013

How to Cite: Sina PejmanMajid MahdaviSaeed BalalaeiGrowth inhibition and Induction of apoptosis in K562 Chronic Myeloid Leukaemia cells by the derivatives of dihydropyrano[c]chromenes family.J Kermanshah Univ Med Sci.17(7):e74458.

Abstract

Background: Recently, the anticancer effect of Chromene derivatives in different cell types has been reported. In this study, we report some derivatives of dihydropyrano[c]chromenes with growth inhibitory and apoptotic activity against K562, Chronic Myeloid Leukaemia (CML).

Methods: The cells were seeded in 96-well plates at 1×105 cells/well and treated with different concentrations of the dihydropyrano[c]chromenes (4-PC, 4-CNC and 4-HC). Growth inhibition and viability determined by trepan blue exclusion test. Apoptosis was also detected by fluorescent microscopy and DNA fragmentation assay.

Results: The 4-PC, 4-CNC and 4-HC inhibited growth and viability of the cells in dose- and time-dependent manner. These compounds were found to be highly active growth inhibitor with IC50 of 240, 180 and 160 μM, respectively. The results of Acridine orange/ethidium bromide (AO/EtBr) double staining also revealed that the compounds induced apoptosis at respective IC50 value after 72 h of treatment.

Conclusion: As the effect of growth inhibition and induction of apoptosis in K562 Chronic Myeloid Leukaemia cells were observed, dihydropyrano[c]chromenes can be proposed as effective agents for more investigation in leukaemia treatment.

Keywords

Apoptosis

Chronic Myeloid Leukaemia

dihydropyrano[c]chromenes

K562 cell

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