Reconstructing Calvarial Bone Lesions Using PHBV Scaffolds and Cord Blood Mesenchymal Stem Cells in Rat

Saeed heidari-keshel; Mostafa Rezaei-Tavirani; Esmaeil Biazar; Nayb ali Ahmadi; Maryam Ebrahimi; Abdolaziz Ronaghi; Gholamreza Behrozi; Reza Rozafzoon

Journal of Kermanshah University of Medical Sciences

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Reconstructing Calvarial Bone Lesions Using PHBV Scaffolds and Cord Blood Mesenchymal Stem Cells in Rat

Author(s):

Saeed heidari-keshel¹,

Mostafa Rezaei-Tavirani^2,*,

Esmaeil Biazar³,

Nayb ali Ahmadi²,

Maryam Ebrahimi²,

Abdolaziz Ronaghi²,

Gholamreza Behrozi²,

Reza Rozafzoon⁴

1Student Research committee, Proteomics Research Center, Faculty of paramedical sciences, Shahid Beheshti University of medical sciences, Tehran, Iran

2Proteomics Research Center, Faculty of paramedical sciences, Shahid Beheshti University of medical sciences, Tehran, Iran

3Department of biomaterial engineering, Tonekabon branch, Islamic azad university, Tonekabon, Iran

4Stem cell preparation Unit, Eye Research center, Farabi Eye Hospital, Tehran University of medical sciences, Tehran, Iran

Journal of Kermanshah University of Medical Sciences:Vol. 16, issue 8; e77271

Published online:Mar 29, 2013

Article type:Research Article

Received:Nov 26, 2012

Accepted:Feb 19, 2013

How to Cite:Saeed heidari-keshelMostafa Rezaei-TaviraniEsmaeil BiazarNayb ali AhmadiMaryam EbrahimiAbdolaziz RonaghiGholamreza BehroziReza Rozafzoonet al.Reconstructing Calvarial Bone Lesions Using PHBV Scaffolds and Cord Blood Mesenchymal Stem Cells in Rat.J Kermanshah Univ Med Sci.16(8):e77271.

Abstract

Background: For tissue engineering of bone, anatomical and operational reconstructions of damaged tissue seem to be vital. This is done via reconstruction of bone and appropriate biological joint with bone tissues of damaged areas. In this study the condition of biodegradable bed Nano fiber PHBV and USSC cells were used to accelerate bone repair of damaged area.

Methods: Hollow nanofabrication scaffold of damageable life was designed as PHBV by electrocy and via determining the best factors such as the kind and amount of solvent, specific volume and rate. The separation of osseous tissue infiltration and evaluating its nature by flow cytometrocical analysis was done. Animal test including USSC as well as PHBV condition in the damaged bone was done in the rat. After 8 weeks the implanted area was analyzed using CT-scan and was sent to histopathology ward. Finally, the rate and quality of reconstruction were determined after H and E coloring.

Results: Histomorphic analysis indicated a statistically significant difference between the experimental group of PHBV, USSC+PHBV and control group. Besides, the histopathologic analysis showed that bone reconstruction rate was high in the area containing USSC and PHBV, compared with area having PHBV and control group and consequently the reconstruction quality of bones and the relationship between the new bone tissues and surrounding bone tissues were high too.

Conclusion: Using PHBR scaffold and USSC together could be useful in the amending of wide range of bone lesion.

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