The effect of morphine on phagocytosis of peritoneal phagocytes of mice

Rahil Eftekhari; Hedayatollah Shirzad; Neda Soleimani; Reza Mirzaei; Abolfazl Khoshdel; Mahmoud Rafieian-Kopaei

Journal of Kermanshah University of Medical Sciences

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The effect of morphine on phagocytosis of peritoneal phagocytes of mice

Author(s):

Rahil Eftekhari¹,

Hedayatollah Shirzad¹,

Neda Soleimani¹,

Reza Mirzaei¹,

Abolfazl Khoshdel²,

Mahmoud Rafieian-Kopaei^3,*

1Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

2Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

3Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

Journal of Kermanshah University of Medical Sciences:Vol. 16, issue 4; e78781

Published online:Nov 29, 2012

Article type:Research Article

Received:Jul 24, 2011

Accepted:Dec 27, 2011

How to Cite:Rahil EftekhariHedayatollah ShirzadNeda SoleimaniReza MirzaeiAbolfazl KhoshdelMahmoud Rafieian-Kopaeiet al.The effect of morphine on phagocytosis of peritoneal phagocytes of mice.J Kermanshah Univ Med Sci.16(4):e78781.

Abstract

Background: There are reports about the increase of severity of infections in patients receiving morphine. Therefore this study was aimed to evaluate the effect of morphine on phagocytic activity of peritoneal phagocytes of mice.

Methods: Fifty six female Balb/c mice, 6-8 weeks old and 28±2 gram weight, were divided into two groups. The case group received 20, 40, 60 and 80 mg/kg morphine on first, second, third and fourth days, respectively, in the morning and evening, and 100 mg/kg (0.5 cc) once a day on days 5 to 10. Control group received 0.5 cc normal saline at the same time. Then, the mice were killed on days 3, 5 and 10. The peritoneal contents aspirated and mixed with sheep red blood cells (SRBC). Ultimately, the number of phagocytes that engulfed SRBC was determined with light microscope, and compared in two groups.

Results: The number of phagocytes engulfed SRBC in case group was significantly less in the tenth day (the third stage of experiment) in comparison with that of control group (P<0.031).

Conclusion: Prolonged administration of morphine (ten days) decreases the activity of peritoneal phagocytes in Balb/c mice. Therefore, it should be used with caution in patients in whom immune suppression is hazardous or use alternative drugs instead.

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