Journal of Kermanshah University of Medical Sciences
Peer-reviewed Medical Quarterly
e-ISSN 2588-2570
p-ISSN 2588-2562
Outline
The effect of uncontrolled stress and morphine consumption on spinal cord development in Wistar rats’ embryo
Authors
Elaheh Sadeghian1, Zohreh Momtaz1, Mehrangiz Sadoughi1, Mahnaz Azarnia2, Hossein Bahadoran3, Mahsa Noroozzadeh1, Hedayat Sahraei4,*
1Dept. of Biology, School of Science, Islamic Azad University, Tehran North Branch, Tehran, Iran
2Dept. of Biology, School of Science, Tarbiat Moalem University, Tehran, Iran
3Dept. of Anatomy, School of Medicien, Baqiyatallah (A.S.) University of Medical Sciences, Tehran, Iran
4Neuroscience Research Center, Baqiyatallah (A.S.) University of Medical Sciences, Tehran, Iran
*Corresponding Author: Corresponding author: Hedayat Sahraei, Neuroscience Research Center, Baqiyatallah (A.S.) University of Medical Sciences, Tehran, Iran, Tel: +98 21 26127257 Email: [email protected]
Published online:Sep 29, 2012
Article type:Research Article
Received:May 23, 2011
Accepted:Nov 29, 2011
How to Cite:Sadeghian E, Momtaz Z, Sadoughi M, Azarnia M, Bahadoran H, et al. The effect of uncontrolled stress and morphine consumption on spinal cord development in Wistar rats’ embryo. J Kermanshah Univ Med Sci. 2012;16(3):e78800. doi:
Abstract
Background: Studies have indicated that morphine may induce its delay effect on embryos development by corticosterone release. In the present study the effect of simultaneous oral morphine consumption and stress on spinal cord development in Wistar rat embryos was investigated.
Methods: Wistar rats (250-300 g) were used in this study. After pregnancy, the animals were divided into control, morphine (0.05 mg in tap water), stress (electro foot shock), and experimental (morphine plus stress) groups. On 14th embryonic day, the rats were killed and their embryos were surgically removed, washed, weighted, and their crown-romp lengths were measured and were fixed in formalin 10%. Then tissue processing, cutting and Hematoxylin- Eosin (H&E) staining were preformed. The samples were evaluated using light microscope and MOTIC software for changes in spinal cord area. Data were analyzed using SPSS software version 9.01.
Result: The weight of embryos was increased in the stress group and decreased in the stress plus morphine group. This was true for the length of the embryos as well. In addition, the area of the spinal cord in the stressed group was increased but in the experimental group decreased
Conclusion: It seems that morphine and stress induced their influence on embryo development via different pathways and it is not true that morphine effect on embryos development is due to its ability for corticosterone release in the pregnant rats.
Highlights
References
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Copyright
© 2012, Journal of Kermanshah University of Medical Sciences. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.
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