Studies of some cancer markers and blood factors in DMBA- induced breast cancer in SD rat strains

Ahmad Hamta; Sayed Mohammad Ali Shariatzadeh; Malek Solimani Mehrnejani

Journal of Kermanshah University of Medical Sciences

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Studies of some cancer markers and blood factors in DMBA- induced breast cancer in SD rat strains

Author(s):

Ahmad Hamta^1,*,

Sayed Mohammad Ali Shariatzadeh¹,

Malek Solimani Mehrnejani¹

1Dept. of Biology, School of Sciences, Arak University, Arak, Iran

Journal of Kermanshah University of Medical Sciences:Vol. 15, issue 3; e79360

Published online:Sep 19, 2011

Article type:Research Article

Received:Jun 08, 2010

Accepted:Nov 02, 2010

How to Cite:Ahmad HamtaSayed Mohammad Ali ShariatzadehMalek Solimani MehrnejaniStudies of some cancer markers and blood factors in DMBA- induced breast cancer in SD rat strains.J Kermanshah Univ Med Sci.15(3):e79360.

Abstract

Background: Animal suitable models are very useful tools in the studies of complicated diseases. In this way we can study the heterogeneity of human genome and also we can minimize the effect of environmental factors in the incidence of cancers. In this study the expression of VWF and PCNA in tumors was determined by immunohistochemistry and also we measured three cancer markers and two blood factors from affected rats.

Methods: We have gavaged 10 mg DMBA dissolved in 0.5 ml sesame oil to the DMBA-treated SD rat strains. After tumor were appeared tumors were used for immunohistochemistry tests. Also we measured three cancer markers and two blood factors according to the manufacturer's protocol and results were analyzed by SPSS software.

Results: Interaction of two antibodies PCNA and VWF with their antigen were more robust in tumors samples compared to controls with control samples. From blood factors only in tumors CA13.5 showed significant expression (p<0.05).

Conclusion: Histopathology and immunohistochemistry results showed that breast cancer by induced DMBA in rats were similar to human breast cancer. The level of CA15.3 in induced tumors showed significantly increase compared to control blood samples, which is similar to behavior of this cancer marker in human breast cancer. But there was no significant association between treated and control samples related to the other four cancer markers and blood factors These results emphasized that DMBA-induced breast cancer in SD rat strains is a suitable model for biological studies on breast cancers.

Keywords

12-dimethylbenz[α] anthracene

breast cancer

SD rat strain

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