The Effect of some Flavonoids on in-vitro Non-enzymatic Glycosylation of Proteins

authors:

avatar S Asgary 1 , * , avatar Gh Naderi 1 , avatar M Gharipoor 1

Iran
Journal of Kermanshah University of Medical Sciences: Vol.8, issue 1; e81306
published online: June 19, 2004
article type: Research Article
received: January 05, 2004
accepted: May 10, 2004

how to cite: Asgary S, Naderi G, Gharipoor M. The Effect of some Flavonoids on in-vitro Non-enzymatic Glycosylation of Proteins. J Kermanshah Univ Med Sci. 2004;8(1):e81306. 

Abstract

Introduction: Non-enzymatic glycosylation of proteins which is the major cause of diabetic complications, cardiovascular diseases retinopathy, nephropathy and neuropathy can effectively be inhibted by antioxidants.
Materials and Method: The antioxidant effects of several flavonoids, such as rutin, kaempferol, quercetin, apigenin, naringin, morin and biochanin A were studied  on glycosylation of insulin, hemoglobin and albuminin In-vitro.
Optimal glucose concentration as well as incubation time were determined and inhibition percentage of the proteins were measured at three different concentrations (0.5,5 and 10 μg/ml) using spectrophotometric method.
Results: This study showed that biochanin A has potent antioxicant effects as it inhibits glycosylation of hemoglobin insulin and albumin by 100%, 60% and 100% respectively.Apigenin is also inhibits albumin glycosylation by 100%.
Conclusion: As potent antioxidant effects are induced by flavenoedes, preventive effects of some plants containing the flavonoids on diabetic complication is expected.

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