Evaluation of Total Phenolics, Flavonoids, and Antioxidant and Cytotoxic Potential of Ailanthus altissima (Mill.) Swingle Leaves

Heba Raafat Mohamed; Eman El-Wakil; El-Sayed Abdel-Hameed; Maher El-Hashash; Mohamed shemis

doi:10.4103/jrptps.JRPTPS_7_21

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https://doi.org/10.4103/jrptps.JRPTPS_7_21

Evaluation of Total Phenolics, Flavonoids, and Antioxidant and Cytotoxic Potential of Ailanthus altissima (Mill.) Swingle Leaves

Author(s):

Heba Raafat Mohamed^1,*,

Eman El-Wakil¹,

El-Sayed Abdel-Hameed¹,

Maher El-Hashash²,

Mohamed shemis³

1Laboratory of Medicinal Chemistry, Theodor Bilharz Research Institute, Giza, Egypt

2Department of Chemistry, Faculty of Science, Ain-Shams University, Cairo, Egypt

3Laboratory of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Giza, Egypt

Journal of Reports in Pharmaceutical Sciences:Vol. 10, issue 1; 130-6

Published online:May 31, 2021

Article type:Research Article

Received:Jan 12, 2021

Accepted:Apr 04, 2021

How to Cite:Heba Raafat MohamedEman El-WakilEl-Sayed Abdel-HameedMaher El-HashashMohamed shemisEvaluation of Total Phenolics, Flavonoids, and Antioxidant and Cytotoxic Potential of Ailanthus altissima (Mill.) Swingle Leaves.J Rep Pharm Sci.10(1):e147103.https://doi.org/10.4103/jrptps.JRPTPS_7_21.

Abstract

Context: People all over the world are suffering from cancer. Liver cancer is considered the second most common malignancy among Egyptian men and the sixth most common malignancy among Egyptian women. Plant-derived antioxidants are believed to prevent or delay the occurrence of many chronic diseases such as cancer. Ailanthus altissima has been used in many traditional prescriptions.
Aims: The current study aimed at investigating the phytochemical profile of A. altissima leaves’ extract and its derived fractions, determining their content of phenolics and flavonoids as well as assessing their antioxidant and cytotoxic potential.
Materials and Methods: The phytochemical screening was carried out using standard methods. The total phenolic, flavonoid, and flavonol contents were determined using Folin-Ciocalteu, aluminum chloride, and aluminum chloride/ sodium acetate assays, respectively. The antioxidant activity was evaluated using different in vitro methods: DPPH•, total antioxidant capacity, hydroxyl (•OH), nitric oxide (NO•) radical scavenging activities, and permanganate-reducing antioxidant capacity (PRAC). The antiproliferative potential against HepG2 cells was evaluated using sulforhodamine-B assay (SRB).
Results: The results showed that the ethyl acetate fraction had the highest content of phenolics, flavonoids, and flavonols (551.72 } 1.81 mg GAE/g ext., 371.24 } 4.36 mg RE/g ext., and 100.47 } 1.30 mg QE/g ext., respectively). It also had the most potent reducing power (DPPH• SC50 = 7.19 } 0.05 μg/mL, TAC= 369.88 } 1.51 mg AAE/g ext., •OH SA = 95.46 } 0.14%, NO• SA = 40.65 } 0.91%, and PRAC = 77.19 } 0.27%). The n-butanol fraction exhibited the most potent cytotoxic potential against HepG2 cells (IC50 = 16.70 μg/mL).
Conclusion: A. altissima leaves could be considered potent antioxidant and cytotoxic alternatives.

Keywords

Ailanthus altissima

antioxidant activity

cytotoxic activity

phytochemical screening

total flavonols

total phenolics

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Author(s):

Heba Raafat Mohamed:[PubMed][Scholar]
Eman El-Wakil:[PubMed][Scholar]
El-Sayed Abdel-Hameed:[PubMed][Scholar]
Maher El-Hashash:[PubMed][Scholar]
Mohamed shemis:[PubMed][Scholar]