Chemical Composition and Antioxidant Activity of Pistacia Atlantica Subsp. Kurdica from Awraman

Seyed Mostafa Nachvak; Seyran Hosseini; Amir Nili-Ahmadabadi; Dara Dastan; Mansour Rezaei

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Chemical Composition and Antioxidant Activity of Pistacia Atlantica Subsp. Kurdica from Awraman

Author(s):

Seyed Mostafa Nachvak¹,

Seyran Hosseini^1,*,

Amir Nili-Ahmadabadi^{2, 3},

Dara Dastan^{2, 4,**},

Mansour Rezaei

⁵

1Department of Nutrition, School of Health, Kermanshah University of Medical Sciences, Kermanshah, Iran

2Medicinal Plants and Natural Products Research Center, Hamadan, Iran

3Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran

4Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran

5Biostatistics and Epidemiology Department, Kermanshah University of Medical Science, Kermanshah, Iran

Corresponding Authors:

Journal of Reports in Pharmaceutical Sciences:Vol. 7, issue 3; 222-230

Published online:May 29, 2018

Article type:Research Article

Received:Mar 09, 2018

Accepted:May 02, 2018

How to Cite:Seyed Mostafa NachvakSeyran HosseiniAmir Nili-AhmadabadiDara DastanMansour RezaeiChemical Composition and Antioxidant Activity of Pistacia Atlantica Subsp. Kurdica from Awraman.J Rep Pharm Sci.7(3):e147487.

Abstract

Pistacia atlantica subsp. kurdica is an important food source and a well-known medicinal plant in Zagros Mountains of Iran. The aim of this study was to evaluate phytochemical composition and biological activity of P. atlantica hulls. The phenolics profile of P. atlantica hulls extract was analyzed using the Reversed-phase high-pressure liquid chromatography equipped with photodiode array detector (RP-HPLC-PDA) and the chemical composition of essential oil was determined using the Gas chromatography–mass spectrometry (GC-MS) and Gas Chromatography – Flame Ionization Detector (GC-FID). The total phenolic and total flavonoid contents of hydro-alcoholic extract of P. atlantica hulls were measured by Folin–Ciocalteu and aluminium chloride colorimetric methods, respectively. The antioxidant activity of hydro-alcoholic extract was studied by using 2,2-diphenyl,1-picrylhydrazyl (DPPH) assay. Quantitative HPLC analysis indicated that ferulic acid, quercetin and naringenin are the main phenolic compounds in plant extract. In total, 55 compounds were identified and the main identified compounds of the essential oil were α-pinene and bornyl acetate. The hydro-alcoholic extract showed moderate antioxidant activity with IC50 =75 μg ml-1. The total phenolic content of extract was 15.9 mg gallic acid equivalents/g dry weight, and the total flavonoid content of extract was 28 mg quercetin /g dry weight. The data of this study suggests that P. atlantica has potential for application as an antioxidant agent in pharmaceutical and food industries.

Keywords

Pistacia Atlantica Subsp. Kurdica

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