Salt-Assisted Liquid-Liquid Extraction followed by High Performance Liquid Chromatography for Determination of Carvedilol in Human Plasma

Alireza Pourhossein; Kamal Alizadeh

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Salt-Assisted Liquid-Liquid Extraction followed by High Performance Liquid Chromatography for Determination of Carvedilol in Human Plasma

Author(s):

Alireza Pourhossein¹,

Kamal Alizadeh^1,*

1Department of Chemistry, Lorestan University, Khorramabad 6813717133, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 7, issue 1; 79-87

Published online:Dec 16, 2017

Article type:Research Article

Received:Oct 05, 2017

Accepted:Dec 10, 2017

How to Cite:Alireza PourhosseinKamal AlizadehSalt-Assisted Liquid-Liquid Extraction followed by High Performance Liquid Chromatography for Determination of Carvedilol in Human Plasma.J Rep Pharm Sci.7(1):e147600.

Abstract

A fast and simple method for extraction of carvedilol in human plasma samples based on salting out assisted liquid-liquid extraction (SALLE) is described. The method involving extraction of carvedilol with water-miscible organic solvent acetonitrile when solvent phase separation occurs using NaCl as a salt. The extracted phase was analyzed by high- performance liquid chromatography with ultra violet detection at 240 nm. The procedure has been optimized with respect to type and amount of salt, volume of sample, extraction solvent and the pH of solution. In the optimal condition the linear calibration range was 5-500 μg L-1 and the correlation coefficient was 0.9965. The limit of detection and limit of quantification were 1.0 μg L-1 and 3.3 μg L-1, respectively and a relative standard deviation of 3.5 % for five replicates were obtained. In spiking experiments on real samples, the average recoveries found by the present method were between 96.0% and 105.0%.

Keywords

Salting out assisted liquid-liquid chromatography

Carvedilol

HPLC

Human Plasma

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