Abstract
This study was designed to examine the interaction of dihydropyranochromene derivative, 2-amino-4-phenyl-5-oxo-4H, 5H-pyrano-[3, 2-c] chromene-3-carbonitrile (4-PC) with calf thymus DNA (ctDNA) by absorption spectroscopy, competitive fluorescence studies, circular dichroism (CD) and viscosity measurements. It was found that 4-PC could interact with ctDNA through non- intercalative mode and the intrinsic binding constant, Kb, for 4-PC with ctDNA was estimated to be 2.74 ×103 M-1. Methylene blue (MB) displacement studies revealed that 4-PC could not replace ctDNA bounded MB, which is indicative of groove binding mode of 4-PC with DNA. Furthermore, 4-PC induced detectable changes in the CD spectrum of ctDNA as well as changes in its viscosity, corroborate the above experimental results.
Keywords
Binding constant Chromone derivative Circular dichroism Groove binding
Copyright
© 2016, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.