Reproductive Parameters in Diabetic Male Rat after Exposure to Cannabis Sativa Hydroalcoholic Extract

authors:

avatar Nahid Lotfi 1 , avatar Mozafar Khazaei ORCID 2 , * , avatar Sayyed Mohammad Ali Shariatzadeh 3 , avatar Malek Solmani Mehranjani 3 , avatar Faramarz Piri 4 , avatar Atefe Ansarian 5

Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran
Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Department of Biology, Faculty of Sciences, University of Arak, Arak, Iran
Kermanshah branch, Islamic Azad University, Kermanshah, Iran
Department of Cardiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
Journal of Reports in Pharmaceutical Sciences: Vol.3, issue 2; 193-200
published online: December 02, 2014
article type: Research Article
received: August 06, 2014
revised: November 05, 2014
accepted: November 15, 2014

How To Cite Lotfi N, Khazaei M, Shariatzadeh S M A, Mehranjani M S, Piri F, et al. Reproductive Parameters in Diabetic Male Rat after Exposure to Cannabis Sativa Hydroalcoholic Extract. J Rep Pharm Sci. 2014;3(2):e147718. 

Abstract

Diabetes is a metabolic disorder inducing undesirable effects on male reproductive system. Cannabis sativa (C. sativa) is a plant that affects diabetes and decreases blood glucose. The present study was aimed to investigate the effect of Cannabis sativa hydroalcoholic extract on sperm parameters in diabetic rats. Thirty five male Wistar rats (210‐240 gr) were divided into five groups: control (distilled water) and four diabetic (60 mg/kg STZ) groups. Diabetic groups were assigned to one diabetic and three experimental groups (receiving 10, 50, and 100 mg/kg extract). Extract was administered interaperitonealy (IP) for 14 days. The animals were weighed and sacrificed at the end of the study. Blood sample was taken and serum testosterone was measured via ELISA method. Also, testes were weighed and sperms were collected from cauda epididymis and analyzed in terms of count, motility and morphology. Histological slides were prepared from testis and seminiferous tubules diameter was determined using Motic camera and software. Data were analyzed using One‐way ANOVA method and P<0.05 was considered statistically significant. The mean of blood glucose in experimental groups indicated a significant decrease compared to diabetic group (P=0.00). The mean of sperms count and motility, percentage of sperms with normal morphology, testosterone hormone level, seminiferous tubules diameter, and final body weight in diabetic and experimental groups decreased significantly compared to control group (P=0.00). Cannabis sativa hydroalcoholic extract can induce a moderating effect on hyperglasemia resulting from diabetes, but has no ameliorating effect on diabetic reproductive dysfunctions.