Cytotoxic Effects of the Essential Oil from Achillea wilhelmsii C. Koch

Toraj Amadi Jouybari; Mohammad Reza Nikbakht; Kamran Mansouri; Bagher Takhtshahi

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Cytotoxic Effects of the Essential Oil from Achillea wilhelmsii C. Koch

Author(s):

Toraj Amadi Jouybari¹,

Mohammad Reza Nikbakht²,

Kamran Mansouri³,

Bagher Takhtshahi^2,*

1Clinical Research Development Center, Imam Khomeini Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran

2Department of Pharmacology and Toxicology, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

3Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 2, issue 2; 98-102

Published online:Jun 20, 2013

Article type:Research Article

Received:May 19, 2013

Accepted:Jun 15, 2013

How to Cite:Toraj Amadi JouybariMohammad Reza NikbakhtKamran MansouriBagher TakhtshahiCytotoxic Effects of the Essential Oil from Achillea wilhelmsii C. Koch.J Rep Pharm Sci.2(2):e147737.

Abstract

The cytotoxicity activity of the essential oils from the leaves of Achillea wilhelmsii C. Koch were studied on six tumor cell lines and a normal cell line with Lactate dehydrogenases (LDH) and trypan blue methods. The composition of the essential oil was also analyzed by Gas Chromatography–Mass spectrophotometery (GC-MS). The LDH test showed that the oil had marked cytotoxicity activity against all cancer cell lines. K562 (Human chronic myelogenous leukemia) and PC3 (Human prostate adenocarcinoma), were the most sensitive to the essential oil with IC50 value (the concentration of the essential oil causing 50% inhibition) of 12.62±1.3μg/ml and 15.88±2.4μg/ml, respectively. The least cytotoxic activity was exhibited on HUVEC (Human umbilical vein endothelial cell) and Hela (Human cervix carcinoma) cell lines with IC50 values of 19.85±3.2 and 46.34±2.7, respectively. Trypan blue assay approved these results. In conclusion, cytotoxic activities of the oil may be described by the presence of monoterpene derivatives. the results of this study suggest that the essential oil of A. wilhelmsii has a potential source for cancer therapy. As a significant result, HUVEC (as normal cells) exhibited the least sensitivity to cytotoxic effects of the oil, confirming its high selectivity and specialty against cancer cell lines.

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