Evaluation of MALAT1 gene expression in AML and ALL cell lines

authors:

avatar javad ahmadi , avatar Saeid Kaviani jebeli ORCID , * , avatar amir atashi

Koomesh: Vol.17, issue 1; 179-186
published online: December 28, 2015
article type: Research Article
received: August 04, 2014
accepted: April 01, 2015

how to cite: ahmadi J, Kaviani jebeli S, atashi A. Evaluation of MALAT1 gene expression in AML and ALL cell lines. koomesh. 2015;17(1):e150779. 

Abstract

 Introduction: Recently, several long non-coding RNAs (lncRNAs) have been identified as being cancer-specific, namely metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), HOTAIR, and ANRIL. Studies have revealed that MALAT1, is up-regulated in many solid tumors and it have important regulatory roles in cancer biology, metastasis and recurrence. Acute myeloid leukemia (AML) and Acute lymphoblastic leukemia (ALL) are cancers of the myeloid and lymphoid blood cells lines, characterized by the rapid growth of abnormal white blood cells that accumulate in the bone marrow and interfere with the production of normal blood cells. Materials and Methods: KG1 and Jurkat cell lines cultured in IMDM and RPMI-1640 mediums respectively. Total RNA extracted from the cell lines and normal bone marrow cells and MALAT1 gene expression was measured by qRT-PCR. Results: MALAT1 gene expression was significantly increased in the KG1cell line (P