Beneficial effects of mesenchymal stem cells treated with low frequency magnetic fields in a rat model of Parkinson’s disease: Evaluation of rotation test and serum BDNF levels

authors:

avatar Manochehr Safari , avatar Majid Jadidi , avatar Hamid Reza Sameni , avatar S. am Zarbakhsh , avatar Ahmad Reza Bandegi , avatar Abbas Ali Vafaei , avatar Saeed Moghadas Biat , *

Koomesh: Vol.17, issue 1; 224-232
published online: December 28, 2015
article type: Research Article
received: September 01, 2014
accepted: June 01, 2015

how to cite: Safari M, Jadidi M, Sameni H R, Zarbakhsh S A, Bandegi A R, et al. Beneficial effects of mesenchymal stem cells treated with low frequency magnetic fields in a rat model of Parkinson’s disease: Evaluation of rotation test and serum BDNF levels. koomesh. 2015;17(1):e150795. 

Abstract

  Introduction: The use of stem cells is one of the ideal methods in treatment of Parkinson;#39s disease (PD). Recently, low-frequency magnetic fields have been used for differentiation and reproduction of stem cells. In this study, we used electromagnetic fields (EMF) impact on bone marrow stem cells and evaluated its effect on the serum levels of brain derived neurotrophic factor (BDNF), a neurotrophic factor with decreased expression in PD.   Materials and Methods: Thirty six male wistar rats were randomly divided into 6 groups: control, PD, PD implanted with stem cells exposed to 40 or 400 microtesla EMFs, implanted with stem cells without EMF exposure and vehicle. Parkinson model was induced by injection of 4 µg neurotoxin 6- OHDA into the left striatum. Stem cells were injected in left lateral ventricle.   Results: Injection of mesenchymal stem cells exposed to 40 and 400 microtesla EMFs significantly reduced amphetamine-induced rotation activity and increased serum BDNF levels in rats. The effect of 400 microtesla EMF was considerably higher than that of 40 microtesla EMF. There was not any significant difference between the effects of mesenchymal cells with 40 microtesla exposure and those without any exposure on the rotation test and serum BDNF levels.  Conclusion: Bone marrow stem cells exposed to 400 microtesla EMF considerably reduced amphetamine-induced rotation activity and increased the serum levels of BDNF in rat