Proteomics Study of extremely low frequency electromagnetic field (50 Hz) on human neuroblastoma cells

authors:

avatar Hadi Hasanzadeh , avatar Mosatafa Rezaei-Tavirani ORCID , * , avatar Samaneh Seyyedi , avatar Alireza Emadi

Koomesh: Vol.17, issue 1; 233-238
article type: issue_documents_importer
received: August 01, 2015
accepted: September 07, 2015

how to cite: Hasanzadeh H, Rezaei-Tavirani M, Seyyedi S, Emadi A. Proteomics Study of extremely low frequency electromagnetic field (50 Hz) on human neuroblastoma cells. koomesh. 2015;17(1):e150796. 

Abstract

  Introduction: Extremely low frequency electromagnetic field (ELF-EMF) is one of common environmental factors in today’s modern life. In recent years, a special interest has been directed toward the possible effects of ELF-EMFs on biological systems. Since the mechanism underlying the ELF-EMFs’ biological effects are still unknown, we decided to conduct an in vitro study to understand the possible mechanisms.   Material and Methods: SH-SY5Y- human neuroblastoma cell line were placed in the EL-EM Field with the intensity of 1 mT and frequency of 50 Hz and for a period of 4 hours/day, in 2 days period. The cell morphology and protein expression were measured by using proteomic methods. Statistical analysis was performed by using Non Linear Spot Progenesis Same software.   Results : Our results showed that ELF-EMF could likely change the cell morphology and proliferation.   Conclusion: Statistical analysis in our study showed that ELF-EMF with a frequency of 50Hz and intensity of 1mT could significantly change the protein expression (P ;le 0.05).