Anti-acetylcholine esterase activity of aqueous extract of lavandula angustifolia and its toxicity effect on HepG2 cell line

authors:

avatar Masoud Soheili , avatar Mostafa Rezaei , avatar Mahmoud Salami , *

Koomesh: Vol.19, issue 2; 263-268
published online: June 06, 2017
article type: Research Article
received: October 02, 2015
accepted: February 06, 2017

how to cite: Soheili M, Rezaei M, Salami M. Anti-acetylcholine esterase activity of aqueous extract of lavandula angustifolia and its toxicity effect on HepG2 cell line. koomesh. 2017;19(2):e151078. 

Abstract

Introduction: Lavandula angustifolia (lavender) is an aromatic evergreen of laminacea family with some medicinal characteristics. The effect of lavender aqueous extract on improving learning and memory in Alzheimeric model of animals has been proved. Reduced acetylcholine due to increased activity of acetylcholine esterase is one of the main symptoms of Alzheimer;#39s disease. This research was accomplished in order to evaluate the anti-acetylcholine esterase activity of the aqueous extract of lavender. Meanwhile, the toxic effect of the herbal medicine on hepatic HepG2 cell line was considered. Materials and Methods: The dried flowers of lavender were mixed with boiled water and then evaporated. In this experimental study the acetylcholine esterase inhibitory activity of lavender was assessed using Ellman’s colorimetric method in 96 well microplates at 405 nm. Also the toxicity effect of lavender was evaluated on HepG2 cell line. Results: Comparing the results taken from the treated and untreated solutions showed that the aqueous extract of lavender did not affect efficiently the acetylcholine esterase inhibitory activity. Also the microscopic evaluation of the HepG2 cells indicated no granulation of the treated cells compared with the untreated cells confirming that the aqueous extract of lavender has no toxic effect on the HepG2 cell line. Conclusion: The aqueous extract of lavender is not affected impressively the acetylcholine esterase activity and also is not toxic to the hepatic cells

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