Effects of 10 weeks endurance exercise on mRNA expressions of angiogenesis factors of skeletal muscle following myocardial infarction in rats

authors:

avatar malihe ardakanizade , avatar kamal ranjbar , avatar farzad nazem , *


how to cite: ardakanizade M, ranjbar K, nazem F. Effects of 10 weeks endurance exercise on mRNA expressions of angiogenesis factors of skeletal muscle following myocardial infarction in rats. koomesh. 2017;19(1):e151112. 

Abstract

Introduction: The effect of exercise training on skeletal muscle angiogenesis in myocardial infarction rats is not yet clear. Therefore, the aim of this study was to investigate the effect of exercise training on the gene expressions of angiogenesis factors in the soleus muscle following myocardial infarction (MI) in rats. Material and Methods: Four weeks after the MI surgical procedures, 30 Wistar male rats were divided to the following groups: sham, MI-control and MI-exercise. The rats were subjected to aerobic training with moderate intensity for 10 weeks. After the last exercise-training session, soleus capillary density and the expression of angiogenic gene factors were measured by immunohistochemistry and RT-PCR, respectively. Results: Capillary density and capillary to fiber ratio (C/F) significantly decreased 14 weeks after MI. Exercise training significantly increased capillary density and C/F ratio in soleus muscle. Furthermore, HIF-1 and TGF-β gene expression respectively increased and decreased, but, VEGF, FGF-2 and angiostatin gene expression did not changed in soleus muscle after training. Conclusion: Results showed that MI significantly decreased soleus muscle capillary density. Furthermore, 10 weeks moderate endurance exercise ameliorate capillary density and C/F ratio, parallel to HIF-1 up regulation, TGF-β down regulation and unchanged VEGF, FGF-2 and angiostatin gene expression in soleus muscle after myocardial infarction.

References

  • 1.

    Brown DA, Jew KN, Sparagna GC, Musch TI, Moore RL. Exercise training preserves coronary flow and reduces infarct size after ischemia-reperfusion in rat heart. J Appl Physiol (1985) 2003; 95: 2510-2518.

  • 2.

    Cheraghian B, Nedjat S, Mansournia MA, Majdzadeh R, Mohammad K, Vaez-Mahdavi MR, et al. Different patterns of association between education and wealth with non-fatal myocardial infarction in Tehran, Iran: A population-based case-control study. Med J Islam Repub Iran 2015; 29: 160.

  • 3.

    Thomas DP, Hudlicka O, Brown MD, Deveci D. Alterations in small arterioles precede changes in limb skeletal muscle after myocardial infarction. Am J Physiol 1998; 275: H1032-H1039.

  • 4.

    Minotti JR, Christoph I, Oka R, Weiner M, Wells L, Massie B. Impaired skeletal muscle function in patients with congestive heart failure. Relationship to systemic exercise performance. J Clin Invest 1991; 88: 2077.

  • 5.

    JC E. Low-frequency electrical stimulation of skeletal muscles in patients with chronic heart failure. Scripta Medica 2002;75:203-208.

  • 6.

    Delp MD, Duan C, Mattson JP, Musch TI. Changes in skeletal muscle biochemistry and histology relative to fiber type in rats with heart failure. J Appl Physiol (1985) 1997; 83: 1291-1299.

  • 7.

    Drexler H, Riede U, Mnzel T, Knig H, Funke E, Just H. Alterations of skeletal muscle in chronic heart failure. Circulation 1992; 85: 1751-1759.

  • 8.

    Ogoh S, Hirai T, Nohara R, Taguchi S. [Adaptation in properties of skeletal muscle to coronary artery occlusion/reperfusion in rats]. Nihon Seirigaku Zasshi 2001; 64: 225-236.

  • 9.

    Nourshahi M, Hedayati M, Ranjbar K. The correlation between resting serum leptin and serum angiogenic indices at rest and after submaximal exercise. Regul Pept 2012; 173: 6-12.

  • 10.

    Yadav L, Puri N, Rastogi V, Satpute P, Sharma V. Tumour angiogenesis and angiogenic inhibitors: a review. J Clin Diagn Res 2015; 9: XE01-XE05.

  • 11.

    Huang D, Lan H, Liu F, Wang S, Chen X, Jin K, Mou X. Anti-angiogenesis or pro-angiogenesis for cancer treatment: focus on drug distribution. Int J Clin Exp Med 2015; 8: 8369-8376.

  • 12.

    Shin KO, Bae JY, Woo J, Jang KS, Kim KS, Park JS, et al. The effect of exercise on expression of myokine and angiogenesis mRNA in skeletal muscle of high fat diet induced obese rat. J Exerc Nutrition Biochem 2015; 19: 91-98.

  • 13.

    Lee I, Httemann M, Kruger A, Bollig-Fischer A, Malek MH. ()Epicatechin combined with 8 weeks of treadmill exercise is associated with increased angiogenic and mitochondrial signaling in mice. Front Pharmacol 2015; 6: 43.

  • 14.

    White FC, Bloor CM, McKirnan MD, Carroll SM. Exercise training in swine promotes growth of arteriolar bed and capillary angiogenesis in heart. J Appl Physiol (1985) 1998; 85: 1160-1168.

  • 15.

    Melo SF, Fernandes T, Barana V, Matos KC, Santos AA, Tucci PJ, Oliveira EM. Expression of microRNA-29 and collagen in cardiac muscle after swimming training in myocardial-infarcted rats. Cell Physiol Biochem 2014; 33: 657-669.

  • 16.

    Leosco D, Rengo G, Iaccarino G, Golino L, Marchese M, Fortunato F, et al. Exercise promotes angiogenesis and improves -adrenergic receptor signalling in the post-ischaemic failing rat heart. Cardiovasc Res 2008; 78: 385-394.

  • 17.

    Ranjbar K, Nazem F, Nazari A. Effect of exercise training and L-arginine on oxidative stress and left ventricular function in the post-ischemic failing rat heart. Cardiovasc Toxicol 2016; 16: 122-129.

  • 18.

    Capoccia BJ, Shepherd RM, Link DC. G-CSF and AMD3100 mobilize monocytes into the blood that stimulate angiogenesis in vivo through a paracrine mechanism. Blood 2006; 108: 2438-2445.

  • 19.

    Veiga ECdA, Portes LA, Bocalini DS, Antonio EL, Santos AAd, Santos MH, Silva FA, Tucci PJF. Cardiac implications after myocardial infarction in rats previously undergoing physical exercise. Arq Bras Cardiol 2013; 100: 37-43.

  • 20.

    Kannus P, Jozsa L, Jrvinen TL, Kvist M, Vieno T, Jrvinen TA, et al. Free mobilization and low-to high-intensity exercise in immobilization-induced muscle atrophy. J Appl Physiol (1985) 1998; 84: 1418-1424.

  • 21.

    Suzuki J. L-arginine supplementation causes additional effects on exercise-induced angiogenesis and VEGF expression in the heart and hind-leg muscles of middle-aged rats. J Physiol Sci 2006; 56: 39-44.

  • 22.

    Lloyd PG, Prior BM, Yang HT, Terjung RL. Angiogenic growth factor expression in rat skeletal muscle in response to exercise training. Am J Physiol Heart Circ Physiol 2003; 284: H1668-H1678.

  • 23.

    Breen E, Johnson E, Wagner H, Tseng H, Sung L, Wagner P. Angiogenic growth factor mRNA responses in muscle to a single bout of exercise. J Appl Physiol (1985) 1996; 81: 355-3561.

  • 24.

    Kimura H, Weisz A, Kurashima Y, Hashimoto K, Ogura T, D'Acquisto F, et al. Hypoxia response element of the human vascular endothelial growth factor gene mediates transcriptional regulation by nitric oxide: control of hypoxia-inducible factor-1 activity by nitric oxide. Blood 2000; 95: 189-197.

  • 25.

    Richardson R, Wagner H, Mudaliar S, Saucedo E, Henry R, Wagner P. Exercise adaptation attenuates VEGF gene expression in human skeletal muscle. Am J Physiol Heart Circ Physiol 2000; 279: H772-H778.

  • 26.

    Olfert IM, Breen EC, Mathieu-Costello O, Wagner PD. Skeletal muscle capillarity and angiogenic mRNA levels after exercise training in normoxia and chronic hypoxia. J Appl Physiol (1985) 2001; 91: 1176-1184.

  • 27.

    Delavar H, Nogueira L, Wagner PD, Hogan MC, Metzger D, Breen EC. Skeletal myofiber VEGF is essential for the exercise training response in adult mice. Am J Physiol Regul Integr Comp Physiol 2014; 306: R586-R595##.