Expression of CD86 Co-stimulatory gene in colon polyps

authors:

avatar Noshad Peyravian , avatar Hamid Assadzadeh-Aghdaei ORCID , * , avatar Ehsan Nazemalhosseini Mojarad , avatar Mehrdad Hashemi , avatar Mohammadreza Razavi

Koomesh: Vol.18, issue 1; 13-24
published online: September 28, 2016
article type: issue_documents_importer
received: October 02, 2015
accepted: April 03, 2016

how to cite: Peyravian N, Assadzadeh-Aghdaei H, Nazemalhosseini Mojarad E, Hashemi M, Razavi M. Expression of CD86 Co-stimulatory gene in colon polyps. koomesh. 2016;18(1):e151141. 

Abstract

Introduction: Since the majority of colorectal cancers originate from polyps in the area, therefore, the investigation of the immunological gene expression in colon polyps can be used as a factor for the detection of genetic and pathological differences in polyps. The aim of this study was investigating the expression of CD86 gene in colorectal polyps and its association with malignancy. Materials and Methods: In a descriptive-analytical study during (2012 to 2014), forty-nine biopsy samples were collected during colonoscopy from the patients with colorectal polyps and ten healthy subjects for normalization. A questionnaire including clinical and demographic data was filled out for all cases. Mucosal mRNA expression of CD86 gene was investigated using Real-Time PCR and Fold Change of gene expression (2-DDCt) method. Results: A total of 49 patients including 27(55.2%) male and 22(44.8%) female with mean age of 53±15.3 were evaluated in this study. Adenoma and hyperplastic polyps were reported in 69.3% and 30.7% patients respectively. More than 90% of adenomatous polyps were spread out to the colon while the others were located in the rectum. Based on Relative Quantitation method, over-expression of CD86 genes in colon polyps were observed (Polyp ≥2-fold normal) and down-expression of CD86 in rectum polyps were noticed in comparison with normal samples (Polyp≤0.5-fold normal). Conclusion: Comparing mRNA expression level of CD86 gene in colon polyps with the profile of mRNA expression of polyps in the rectum indicates the presence of distinct molecular and immunological mRNA expression between polyps of these two sites.

References

  • 1.

    Deschoolmeester V, Baay M, Specenier P, Lardon F, Vermorken JB. A review of the most promising biomarkers in colorectal cancer: one step closer to targeted therapy. Oncologist 2010; 15: 699-731.

  • 2.

    Fong TV, Chuah SK, Chiou SS, Chiu KW, Hsu CC, Chiu YC, et al. Correlation of the morphology and size of colonic polyps with their histology. Chang Gung Med J 2003; 26: 339-343.

  • 3.

    Wexner SD, Cohen SM, Ulrich A, Reissman P. Laparoscopic colorectal surgeryare we being honest with our patients? Dise Colon Rectum 1995; 38: 723-727.

  • 4.

    Jia Y, Guo M. Epigenetic changes in colorectal cancer. Chinese J Cancer 2013; 32: 21-30.

  • 5.

    Hoseini S, Moaddabshoar L, Hemati S, MohammadianpanahM. An overview of clinical and pathological characteristics and survival rate of colorectal cancer in Iran. Ann Colorectal Res 2014; 2: e17264.

  • 6.

    Nazemalhosseini Mojarad E, Kuppen PJ, Aghdaei HA, Zali MR. The CpG island methylator phenotype (CIMP) in colorectal cancer. Gastroenterol Hepatol Bed Bench 2013; 6: 120-128.

  • 7.

    Fang SH, Efron JE, Berho ME, Wexner SD. Dilemma of stage II colon cancer and decision making for adjuvant chemotherapy. J Am Coll Surg 2014; 219: 1056-1069.

  • 8.

    Koido S, Ohkusa T, Homma S, Namiki Y, Takakura K, Saito K, et al. Immunotherapy for colorectal cancer. World J Gastroenterol 2013; 19: 8531-8542.

  • 9.

    Ohtani H. Focus on TILs: prognostic significance of tumor infiltrating lymphocytes in human colorectal cancer. Cancer Immun 2007; 7: 4.

  • 10.

    Hu JY, Wang S, Zhu JG, Zhou GH, Sun QB. Expression of B7 costimulation molecules by colorectal cancer cells reduces tumorigenicity and induces anti-tumor immunity. World J Gastroenterol 1999; 5: 147-151.

  • 11.

    Lenschow DJ, Walunas TL, Bluestone JA. CD28/B7 system of T cell costimulation. Annu Rev Immunol 1996; 14: 233-258.

  • 12.

    van der Merwe PA, Bodian DL, Daenke S, Linsley P, DavisSJ. CD80 (B7-1) binds both CD28 and CTLA-4 with a low affinity and very fast kinetics. J Exp Med 1997; 185: 393-404.

  • 13.

    Vasu C, Wang A, Gorla SR, Kaithamana S, Prabhakar BS, Holterman MJ. CD80 and CD86 C domains play an important role in receptor binding and costimulatory properties. Int Immunol 2003; 15: 167-175.

  • 14.

    Zheng Y, Manzotti CN, Liu M, Burke F, Mead KI, Sansom DM. CD86 and CD80 differentially modulate the suppressive function of human regulatory T cells. J Immunol 2004; 172: 2778-2784.

  • 15.

    Rutkowski R, Moniuszko T, Stasiak-Barmuta A, Kosztyla-Hojna B, Alifier M, Rutkowski K, Tatarczuk-Krawiel A. CD80 and CD86 expression on LPS-stimulated monocytes and the effect of CD80 and CD86 blockade on IL-4 and IFN-gamma production in nanotopic bronchial asthma. Arch Immunol Ther Exp (Warsz) 2003; 51: 421-428.

  • 16.

    Folzenlogen D, Hofer M, Leung D, Freed J, Newell M. Analysis of CD80 and CD86 expression on peripheral blood B lymphocytes reveals increased expression of CD86 in lupus patients. Clin Immunol Immunopathol 1997; 83: 199-204.

  • 17.

    Fouda EM, El-Fattah S, El Halim A. Expression of CD80 and CD86 on T lymphocytes and monocytes of asthmaticchildren. Egy J Pediatric Allergy Immunol 2014; 1: 46-53.

  • 18.

    Saada JI, Pinchuk IV, Barrera CA, Adegboyega PA, Suarez G, Mifflin RC, et al. Subepithelial myofibroblasts are novel nonprofessional APCsin the human colonic mucosa. J Immunol 2006; 177: 5968-5979.

  • 19.

    Azimzadeh P, Romani S, Mirtalebi H, Fatemi SR, Kazemian S, Khanyaghma M, Mohebbi SR. Association of co-stimulatory human B-lymphocyte antigen B7-2 (CD86) gene polymorphism with colorectal cancer risk. Gastroenterol Hepatol Bed Bench 2013; 6: 86-91.

  • 20.

    Geng P, Zhao X, Xiang L, Liao Y, Wang N, Ou J, et al. Distinct role of CD86 polymorphisms (rs1129055, rs17281995) in risk of cancer: evidence from a meta-analysis. Plos ONE 2014; 9: 1.

  • 21.

    Pan XM, Gao LB, Liang WB, Liu Y, Zhu Y, Tang M, et al. CD86+ 1057 G/A polymorphism and the risk of colorectal cancer. DNA Cell Biol 2010; 29: 381-386.

  • 22.

    Scarpa M, Behboo R, Angriman I, Termini B, Barollo M, Ruffolo C, et al. The role of costimulatory molecules CD80 and CD86 and IFN in the pathogenesis of ulcerative colitis. Dig Dis Sci 2004; 49: 1738-1744.

  • 23.

    Scarpa M, Bortolami M, Cecchetto A, Faggian D, Kotsafti A, Ruffolo C, et al. Mucosal immune environment in colonic carcinogenesis: CD80 up-regulation in colonic dysplasia in ulcerative colitis. Eur J Cancer 2011; 47: 611-619.

  • 24.

    Hu N, Qian L, Hu Y, Shou JZ, Wang C, Giffen C, et al. Quantitative real-time RT-PCR validation of differential mRNA expression of SPARC, FADD, Fascin, COL7A1, CK4, TGM3, ECM1, PPL and EVPL in esophageal squamous cell carcinoma. BMC Cancer 2006; 6: 1.

  • 25.

    Salazar R, Roepman P, Capella G, Moreno V, Simon I, Dreezen C, et al. Gene expression signature to improve prognosis prediction of stage II and III colorectal cancer. J Clin Oncol 2010; 29: 17-247.

  • 26.

    Goldstein NS, Bhanot P, Odish E, Hunter S. Hyperplastic-like colon polyps that preceded microsatellite-unstable adenocarcinomas. Am J Clin Pathol 2003; 119: 778-796.

  • 27.

    EshghiMJ, Fatemi R, Hashemy A, Aldulaimi D, Khodadoostan M. A retrospective study of patients with colorectal polyps. Gastroenterol Hepatol Bed Bench 2010; 4: 17-22.

  • 28.

    Zhang B, Shrubsole MJ, Li G, Cai Q, Edwards T, Smalley WE, et al. Association of genetic variants for colorectal cancer differs by subtypes of polyps in the colorectum. Carcinogenesis 2012; 33: 2417-2423.

  • 29.

    Galon J, Mlecnik B, Bindea G, Angell HK, Berger A, Lagorce C, et al. Towards the introduction of the Immunoscore in the classification of malignant tumours. J Pathol 2014; 232: 199-209.

  • 30.

    Kashfi SM, Mojarad EN, Pourhoseingholi MA, Aghdaei HA, Anaraki F, Zali MR. Evaluation of the left-to-right shift of colon tumors in Iran: Is the trend changing? J Res Med Sci 2015; 20: 978-986##.