Effects of dietary conjugated linoleic acid on serum visfatin level and tumor number and size in rat model of colorectal cancer

authors:

avatar Zahra kamal , avatar Marjan Ajami , avatar Nasrin Beheshti , avatar Morteza Abdollahi , avatar Sayed Hosein Davoodi ORCID , *

Koomesh: Vol.18, issue 1; 165-173
published online: September 28, 2016
article type: Research Article
received: February 01, 2016
accepted: July 02, 2016

how to cite: kamal Z, Ajami M, Beheshti N, Abdollahi M, Davoodi S H. Effects of dietary conjugated linoleic acid on serum visfatin level and tumor number and size in rat model of colorectal cancer. koomesh. 2016;18(1):e151159. 

Abstract

Introduction: Colorectal cancer is the third causes of cancer death in the world. Recent studies have shown that the serum levels of visfatin are increased during malignancies, suugesting a role for vistatin in pathogenegis of malignancies. It is suggested that conjugated linoleic acid, (CLA) can reduce the serum levels of vistatin. The aim of this syudy was to study the effects of dietary conjugated linoleic acid on serum visfatin level in rat model of colorectal cancer. Materials and Methods: Sixteen male Wistar rats, weighing 250-300 g were divided into two groups: control and experimental. Both groups were injected subcutaneously, with 1,2- Dimethyl Hydrazine at a dose of 15 mg/kg of body weight, twice per week for 6 weeks. The control group and experimental groups were gavaged with water (2 ml/kg) and CLA (200 mg/kg), respectively, for the first 4 weeks of study. After 6 weeks, the rats were killed and their blood and colorectal tissues were collected in order to analyze serum visfatin, tumor number and tumor size respectively by ELISA method, tumor numbering and macroscopic analysing. Results: Visfatin levels decreased dramatically in the experimental group than control group (P=0.001). Tumor number and tumor size increased considerably in the control group than experimental group (P = 0.000). There was a positive correlation between visfatin levels with tumor number (r =0.701, p = 0.001) and also between plasma visfatin with tumor number×tumor size (r=0.771, p=0.000). Conclusion: This study shows that dietary CLA by reducing serum visfatin can decrease the tumor number and tumor size in rat model of colorectal cancer.  Thus, CLA suppresses colon carcinogenesis by a mechanim probably involving reduced serum visfatin

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