Cancer cell detection using electrochemical nanobiosensor based on graphene / gold nanoparticle

authors:

avatar meisam omidi , * , avatar Amir Yadegari , avatar Hakimeh Zali , avatar Mohadese Hashemi , avatar Hadi Hasanzadeh

Koomesh: Vol.18, issue 1; 211-219
published online: September 28, 2016
article type: Research Article
received: November 02, 2015
accepted: June 05, 2016

how to cite: omidi M, Yadegari A, Zali H, Hashemi M, Hasanzadeh H. Cancer cell detection using electrochemical nanobiosensor based on graphene / gold nanoparticle. koomesh. 2016;18(1):e151165. 

Abstract

Introduction: Nowadays, early cancer detection and effective treatment is crucial for improved prognosis and cancer management. In particular, the accurate qualitative detection of cancer cells represents a critical step in cancer diagnosis. The aim of this study was to examine Cancer cell detection using electrochemical nanobiosensor based on graphene / gold nanoparticle. Materials and Methods: Modified graphene oxide/gold nanoparticle electrodes were employed to increase the sensitivity of human breast cancer MCF-7 cells detection, using CD44 biomarker. Frist the electrodes were modified with graphene, then gold nanoparticles were sediment on graphene-modified electrode. Then CD44 monoclonal antibody conjugated on the surface of gold nanoparticles, on graphene-modified electrode. Finally, the performance of the fabricated biosensors were investigated by using a common reference electrode composed of silver-silver chloride and a common platinum counter electrode at different antigen concentrations with the buffer and serum. Results: The proposed electrochemical cytosensor delivered a high sensitivity with the average of 1.12 μA / cells ml-1, and a low detection limit of 6 cells. Conclusion: These results indicate that the cytosensor has great potential in diagnosis of cancers

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