Toxocariasis antibody tittering in patients with uveitis

authors:

avatar sodabeh einipour , avatar fatemeh jaloosian , avatar alireza ramezani , avatar alireza abadi , avatar fatemeh hagialiani , avatar soheila zeinali , avatar Dr Farid Tahvildar , *

Koomesh: Vol.17, issue 4; 903-909
published online: September 28, 2016
article type: Research Article
received: August 01, 2015
accepted: January 01, 2016

how to cite: einipour S, jaloosian F, ramezani A, abadi A, hagialiani F, et al. Toxocariasis antibody tittering in patients with uveitis. koomesh. 2016;17(4):e151183. 

Abstract

Introduction: Toxocariasis is a zoonotic disease caused by the infection of human with second-stage larvae of Toxocaraspecies. The three main human syndromes are visceral larva migrans (VLM), ocular larva migrans (OLM), and covert toxocariasis. The aim of this study was to determine the anti body titer against Toxocara in patients with uveitis. Materials and Methods: A total of 161 uveitis patient’s blood ;Serum were collected from patients referred to training hospitals Universities in Tehran. CBC was performed to determine the presence of hyper eosinophilia. Sera was examined by ELISA to determine Toxocaracanis antibody titer. The positive sera samples were confirmed by westernblot test. Results: One hundred and twenty four uveitis patients were male and 127 were female. Sera samples (n=10, %6/2) were positive for T.canis IgG, including 9 (%7/3) male samples and 1(%2/7) female sample. Nine of sera samples from 10 ELSA positive samples were confirmed for 24-35 KDa bands on nitrocellulose membrane that reconfirmed the Toxocaracanis infection and 1 sample was negative. Conclusion: The mentioned results imply that the ocular toxocariasis exists in Iran and the children are more vulnerable to the disease. Therefore, its necessary to measure the antibody against Toxocaracanis in uveit patients by ELISA test and positive patients should be treated before implicating with irreversible signs and symptoms.

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