Morphine withdrawal symptoms improvement by Ziziphora tenuior L. ethanoic extract in male mice

authors:

avatar Mojtaba Godarzi , avatar Saeid Abbasi Maleki , * , avatar Seyed Zahra Mousavi , avatar Nasroallah Moradi Kor

Koomesh: Vol.17, issue 4; 990-995
published online: September 28, 2016
article type: Research Article
received: May 01, 2015
accepted: March 05, 2016

how to cite: Godarzi M, Abbasi Maleki S, Mousavi S Z, Moradi Kor N. Morphine withdrawal symptoms improvement by Ziziphora tenuior L. ethanoic extract in male mice. koomesh. 2016;17(4):e151194. 

Abstract

Introduction: Ziziphora tenuior L. (Z. tenuior) has multiple biological activities such as spasmolytic, antidiarrheal, analgesic, antidepressant and sedative effects. In the present study, the effect of ethanolic extract of Z. tenuior on morphine withdrawal symptoms was investigated in the male mice. Materials and Methods: In this study, 40 male mice weighing 20-30 g were used. The animals were divided into five groups of eight. The study groups included negative control or normal saline (10 ml/kg), positive control or clonidine (3.5 mg/kg), and different doses of Z. tenuior (50, 75, and 100 mg/kg). Dependency on morphine was induced by administration of different doses of morphine (50, 50, 75, and 50 mg/kg) in four days. On last day, after administration of single dose of morphine, naloxone (5 mg /kg) was injected and morphine withdrawal signs were observed and recorded within 30 min. All drugs and extracts were injected intraperitoneally with a single volume of 10 ml/kg. Results: Results showed that at all doses (50, 75 and 100 mg/kg) of Z. tenuior extract, significantly decreased the number of jumping (P

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