Characterization of neuraminidase gene of H9N2 influenza virus isolated from recent outbreaks in Iran

Sara Samadi; Mahdi Kianizadeh; Reza Toroghi; Seyed Davood Mousavi Nasab; Zahra Gohari; Amir Mohammad Hosseinnia Davatgar; Somayeh Dorri Ardabili; Nayebali Ahmadi

Koomesh

Journal of Semnan University of Medical Sciences

All Issues In Press Accepted Manuscripts Search

Journal Information Editors & Boards Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Publication Malpractice Statement Reviewer and AE Registration Form Support Contact Us

APC

Authors Guide Submit Manuscript

Characterization of neuraminidase gene of H9N2 influenza virus isolated from recent outbreaks in Iran

Author(s):

Sara Samadi,

Mahdi Kianizadeh,

Reza Toroghi,

Seyed Davood Mousavi Nasab,

Zahra Gohari,

Amir Mohammad Hosseinnia Davatgar,

Somayeh Dorri Ardabili,

Nayebali Ahmadi^,*

Koomesh:Vol. 16, issue 4; 512-519

Published online:Sep 28, 2015

Article type:Research Article

Received:Jan 02, 2015

Accepted:Apr 01, 2015

How to Cite:Samadi S, Kianizadeh M, Toroghi R, Mousavi Nasab SD, Gohari Z, et al. Characterization of neuraminidase gene of H9N2 influenza virus isolated from recent outbreaks in Iran. koomesh. 2015;16(4):e151235. doi:

Abstract

Introduction: Influenza viruses cause a variety of infections from high to low pathogenicity in hosts. Influenza virus Ais divided into subtypes based on two surface glycoproteins: the hemagglutinin (H) and the neuraminidase (N). Avian flu viruses do not normally infect humans, although, some sporadic human infections have been reported. The aim of this study was to identify and sequence the neuraminidase gene of Avian Influenza (AI) H9N2 virus isolated from recent outbreaks in Iran and comparative analyzing it with strains from other countries. Materials and Methods: Influenza viruses were passaged in embryonated eggs, and RNA extracted by RNX. TM kit. Using RT- PCR technique, cDNA preparation and amplification of N gene were done. The product was then assessed by electrophoresis and observed with UV, purified and sequenced. Results: Neuraminidase gene nucleotide sequence from six isolates from different parts of Iran were identified and recorded at the GenBank. Comparative analysis with reference strains carried out for these sequences. No mutation had been observed in cytoplasmic domain, however, four mutations had been occurred at transmembrane domain, which two of them were silent and two were actually substitution of hydrophobic amino acids with similar ones. Conclusion: Nucleotide and amino acid analysis for the neuraminidase gene of H9N2 influenza virus of isolates obtained from different parts of Iran revealed similarities to other strains described from neighboring countries, such as Pakistan and Saudi Arabia. It seems like that they have common source with the same lineages.

Keywords

Import into EndNote Import into BibTex

Share on

Comments

Number of Comments:0

Cited by

Scopus by DOI: 0
Last Update: 0 months ago
Scopus by Title: 0
Last Update: 0 months ago
Scopus by Title (Ref): 0
Last Update: 0 months ago
CrossRef: 0
Last Update: 2 days ago

Metrics

Ordering Reprints

Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC

Search Relations

Author(s):

Sara Samadi:[PubMed][Scholar]
Mahdi Kianizadeh:[PubMed][Scholar]
Reza Toroghi:[PubMed][Scholar]
Seyed Davood Mousavi Nasab:[PubMed][Scholar]
Zahra Gohari:[PubMed][Scholar]
Amir Mohammad Hosseinnia Davatgar:[PubMed][Scholar]
Somayeh Dorri Ardabili:[PubMed][Scholar]
Nayebali Ahmadi:[PubMed][Scholar]

Journal of Semnan University of Medical Sciences

Outlines

Characterization of neuraminidase gene of H9N2 influenza virus isolated from recent outbreaks in Iran

Abstract

Last Update: 0 months ago

Last Update: 0 months ago

Last Update: 0 months ago

Last Update: 2 days ago