Isolation and identification of mesenchymal and neural crest characteristics of dental pulp derived stem cells

nazila niapour; zahra tagipour; hossein salehi; abolfazl bagheri; anahita rahani; mahdie talebi; nowrouz najafzadeh; ali niapour

Koomesh

Journal of Semnan University of Medical Sciences

Current Issue Archive In Press Accepted Manuscripts Search

Journal Information Editors & Boards Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Publication Malpractice Statement Support Contact Us Reviewer and AE Registration Form

APC

Authors Guide Submit Manuscript

Isolation and identification of mesenchymal and neural crest characteristics of dental pulp derived stem cells

Author(s):

nazila niapour,

zahra tagipour,

hossein salehi,

abolfazl bagheri,

anahita rahani,

mahdie talebi,

nowrouz najafzadeh,

ali niapour^,*

Koomesh:Vol. 16, issue 4; 520-526

Published online:Sep 28, 2015

Article type:Research Article

Received:Aug 04, 2014

Accepted:Dec 02, 2014

How to Cite:nazila niapourzahra tagipourhossein salehiabolfazl bagherianahita rahanimahdie talebinowrouz najafzadehali niapouret al.Isolation and identification of mesenchymal and neural crest characteristics of dental pulp derived stem cells.koomesh.16(4):e151236.

Abstract

Introduction: Mesenchymal stem cells (MSC) are considered as a unique source of adult stem cells which are usually accessible with minimum ethical concerns. MSCs can be used in stem cell biology , different to other cells, tissue engineering, regenerative medicine and future of cancer treatment researches. The aim of the present study was to isolate dental pulp derived stem cells (DPSCs) , characterizing them with common techniques and determining neural crest gene s expression profile. Materials and Methods: After collecting the teeth , the pulp chamber exposed and the pulp tissue digested with collagenase IV. Dental pulp derived cells were resuspended and incubated in alpha modified minimum essential medium eagle ( αMEM ) supplemented with 15 % fetal bovine serum at 37°C temperature, 95% humidity and 5% CO2 . The osteogenic and adipogenic differentiation potentials for dental pulp derived cells were assessed in appropriate conditions. Flow-cytometry analyses were utilized to evaluate the percentage of CD90 and CD45 positive markers within isolated cells . The expression of p75ngfr, slug and sox10 genes was measured via RT-PCR. Results: Dental pulp derived cells were appropriately attached to the culture vessels and showed high proliferation rate on culture medium . Cells could also differentiate to osteogenic and adipose cells displayed by Alizarin red and Oil red staining . Higher percentage of pulp derived cells were CD90 positive , while the percentage of CD45 expression, as a marker of hematopoietic cells, was negligible . Furthermore , the RT-PCR also revealed the expression of p75ngfr, slug and sox10 in the MSCs isolated from the dental pulps. Conclusion: Dental pulp derived cell have the mesenchymal stem cells characteristics and at least a portion of them have neural crest origin and identity

Keywords

Mesenchymal Stromal cells

Dental pulp

Cell differentiation

Neural Crest

سلول‌های بنیادی مزانشیمی

پالپ دندان

تمایز سلولی

ستیغ عصبی

comments