The metabonomic changes of leishmania major, s promastigotes (fredlin strain) after in vitro artemisinin treatment at stationary phase

Masoud Beigi boroujeni; Mohammad Arjmand; Ghader Khalili; Ziba Akbari; A. li Najafi; Nasim Beigi boroujeni; Afsaneh Shafiei; Reza Haji hosseini

Koomesh

Journal of Semnan University of Medical Sciences

All Issues In Press Accepted Manuscripts Search

Journal Information Editors & Boards Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Publication Malpractice Statement Reviewer and AE Registration Form Support Contact Us

APC

Authors Guide Submit Manuscript

The metabonomic changes of leishmania major, s promastigotes (fredlin strain) after in vitro artemisinin treatment at stationary phase

Author(s):

Masoud Beigi boroujeni,

Mohammad Arjmand^,*,

Ghader Khalili,

Ziba Akbari,

A. li Najafi,

Nasim Beigi boroujeni,

Afsaneh Shafiei,

Reza Haji hosseini

Koomesh:Vol. 16, issue 1; 90-96

Published online:Sep 28, 2014

Article type:Research Article

Received:Dec 02, 2013

Accepted:Jun 08, 2014

How to Cite:Beigi boroujeni M, Arjmand M, Khalili G, Akbari Z, Najafi AL, et al. The metabonomic changes of leishmania major, s promastigotes (fredlin strain) after in vitro artemisinin treatment at stationary phase. koomesh. 2014;16(1):e151285. doi:

Abstract

Introduction: Based on WHO classifications, leishmaniasis enumerates as one of the six important diseases in the tropics. It is also common in the Middle East and the Mediterranean countries. Control of the disease is important due to increased clinical cases in immune-system deficiency patients. With regard to the increasing need to new drugs with lesser side effects for the control and treatment of the disease, we surveyed the in vitro effects of artemisinin on the promastigotes of leishmania major, s metabolites in Pasteur institute of Iran. Materials and Methods: After IC50 determination, the effects of different concentrations of artemisinin drug were assessed on the metabolites of leishmania in two groups the control group (without drug reception in culture media) and the case group (with drug reception in culture media), by using NMR technique. Results: The results of this study indicate that in the metabolic changes in promastigotes of leishmania major (fredlin strain) at stationary phase, 5 metabolites of the 41 ones in the galactose metabolic cycle have shown significant changes. Therefore, galactose metabolism has changed the most, while sphingolipid, valine, leucine and isoleucine biosynthesis follow respectively. Conclusion: Artemisinin resulted in metabolic changes in biochemical pathways including galactose, sphingolipids and also valine, lucine, isoleucine that culminated in cessation of leishmania major’s (fredlin strain) activity. Spectra derived from H NMR showed significant difference (p

Keywords

Import into EndNote Import into BibTex

Share on

Comments

Number of Comments:0

Cited by

Scopus by DOI: 0
Last Update: 1 month ago
Scopus by Title: 1
Last Update: 1 month ago
Scopus by Title (Ref): 1
Last Update: 1 month ago
CrossRef: 0
Last Update: 3 days ago

Metrics

Ordering Reprints

Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC

Search Relations

Author(s):

Masoud Beigi boroujeni:[PubMed][Scholar]
Mohammad Arjmand:[PubMed][Scholar]
Ghader Khalili:[PubMed][Scholar]
Ziba Akbari:[PubMed][Scholar]
A. li Najafi:[PubMed][Scholar]
Nasim Beigi boroujeni:[PubMed][Scholar]
Afsaneh Shafiei:[PubMed][Scholar]
Reza Haji hosseini:[PubMed][Scholar]

Journal of Semnan University of Medical Sciences

Outlines

The metabonomic changes of leishmania major, s promastigotes (fredlin strain) after in vitro artemisinin treatment at stationary phase

Abstract

Last Update: 1 month ago

Last Update: 1 month ago

Last Update: 1 month ago

Last Update: 3 days ago